LOCUS       sequence-37308-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, 
            Cuervo AM, Verkhusha VV
  TITLE     Monomeric fluorescent timers that change color from blue to red 
            report on cellular trafficking.
  JOURNAL   Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.
  PUBMED    19136976
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-02"; volume: "5(2)"; pages: "118-26. Epub 2009 
            Jan 11"
COMMENT     Sequence Label: pTRE-Fast-FT
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..765
                     /codon_start=1
                     /product="blue-to-red fluorescent timer derivative of 
                     mCherry (Subach et al., 2009)"
                     /label=Fast-FT
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHVEGSVNGHEFEIEGEGKGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSRAYVKHPADIPDYWKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERS
                     EGRHSTGGMDEPVQV"
     primer_bind     complement(199..217)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     467..486
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(797..816)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttcatggtga
       61 gcaagggcga ggaggataac atggccatca tcaaagagtt catgcgtttc aaagtgcacg
      121 tggagggttc cgtgaacggc cacgagttcg agatcgaggg cgagggcaag ggccgcccct
      181 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      241 gggacatcct gtcccctcag ttcatgtacg gctccagggc ctacgtgaag catcccgccg
      301 acatccccga ctactggaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      361 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      421 tcatctacaa ggtgaagctg cgcggaacta acttcccctc cgacggcccc gtaatgcaga
      481 agaagaccat gggctgggag gcctccaccg agcggatgta ccccgaggac ggcgccctga
      541 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
      601 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcttacaac gtcaatatca
      661 agttggacat cacatcccac aacgaggact acaccatcgt ggaacagtac gaacgctccg
      721 agggccgcca ctccaccggc ggcatggacg agcctgtaca agtaatctag aggatccaga
      781 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt g
//
