LOCUS       sequence-373115-       10330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express in E. coli human Naa80 delta (actin's N-terminal acetyl 
            transferase) with N-terminal intein-chitin binding domain tag and 
            6xHis tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Ceron RH, Carman PJ, Rebowski G, Boczkowska M, Heuckeroth RO, 
            Dominguez R
  TITLE     A solution to the long-standing problem of actin expression and 
            purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2209150119. doi: 
            10.1073/pnas.2209150119. Epub 2022 Oct 5.
  PUBMED    36197995
REFERENCE   2  (bases 1 to 10330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-11"; volume: "119(41):e2209150119. 
            doi"; pages: " 10.1073/pnas.2209150119. Epub 2022 Oct 5"
COMMENT     Sequence Label: His_Naa80d in pTYB12
FEATURES             Location/Qualifiers
     source          1..10330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(62..148)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     212..228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    244..343
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(637..642,643..660,661..667)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             721..1380
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(787..806)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1722..2027
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1906..1925
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2291..2390)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2408..2426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2431..2447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2431..2447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(2515..2889)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2908..2955)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     3483..3502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3736..3753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3775..3794)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3935..3957
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3951..4142)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     5744..5763
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5783..5860)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     6066..6085
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        6169..6187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6188..6212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6227..6249
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6302..7120
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             7158..7175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7951..7969)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7965..8012
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(8200..8216)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8395..8412)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      8565..8651
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8743..8770
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        8789..8880
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9099..9118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9653..10166)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9848..9869)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10060..10079
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
       61 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      121 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      181 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
      241 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
      301 caatgctttt ttataatgcc aactttgtac aaaaaagcag gctccgcggc cgcccccttc
      361 acctataggg gggaaaaaaa ggtagtcatc agatatatat tttggtaaga aaatatagaa
      421 atgaataatt tcacgtttaa cgaagaggag atgacgtgtg ttccttcgaa cccgagtttt
      481 gttcgtctat aaatagcacc ttctcttctc cttcttcctc acttccatct ttttagcttc
      541 actatctctc tataatcggt tttatctttc tctaagtcac aacccaaaaa aacaaagtag
      601 agaagaatct gtaagagacc attaggcacc ccaggcttta cactttatgc ttccggctcg
      661 tataatgtgt ggattttgag ttaggagccg tcgagatttt caggagctaa ggaagctaaa
      721 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
      781 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      841 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      901 cacattcttg cccgcctgat gaatgctcat ccggagttcc gtatggcaat gaaagacggt
      961 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     1021 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     1081 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     1141 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     1201 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     1261 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     1321 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     1381 acgcgtggag ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     1441 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
     1501 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
     1561 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
     1621 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
     1681 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca
     1741 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     1801 cgcccggccg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
     1861 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     1921 atatggccag tgtgccggtt tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     1981 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
     2041 ttatacacag ccagtctgca cctcgacggt ctcacattgg ctcttcttac tacaatgaaa
     2101 aaggccgagg caaaacgcct aaaatcactt gagaatcaat tctttttact gtccatttaa
     2161 gctatctttt ataaacgtgt cttattttct atctcttttg tttaaactaa gaaactatag
     2221 tattttgtct aaaacaaaac atgaaagaac agattagatc tcatctttag tctcaagggt
     2281 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     2341 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     2401 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     2461 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgatcagtc
     2521 ctgctcctcg gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg
     2581 cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt
     2641 ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc
     2701 cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc
     2761 ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt
     2821 ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa
     2881 cttggccatg gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga
     2941 tgattaattg tcaacacgtg ctgatcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3421 tacctacagt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3541 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3601 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3661 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3721 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcta tggtgcactc tcagtacaat
     3781 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     3841 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     3901 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     3961 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtgc
     4021 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     4081 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     4141 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     4201 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     4261 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     4321 tgccagccga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg
     4381 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg
     4441 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg
     4501 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag
     4561 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact
     4621 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
     4681 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4741 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggn
     4801 tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag
     4861 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg
     4921 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga
     4981 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
     5041 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
     5101 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
     5161 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
     5221 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
     5281 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
     5341 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
     5401 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
     5461 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
     5521 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
     5581 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
     5641 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
     5701 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
     5761 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc
     5821 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc
     5881 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     5941 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     6001 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     6061 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     6121 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
     6181 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
     6241 agaaggagat atacttatga aaatcgaaga aggtaaactg gtaatcggtt ccctggaggg
     6301 ttgctttgcc aagggtacca atgttttaat ggcggatggg tctattgaat gtattgaaaa
     6361 cattgaggtt ggtaataagg tcatgggtaa agatggcaga cctcgtgagg taattaaatt
     6421 gcccagagga agagaaacta tgtacagcgt cgtgcagaaa agtcagcaca gagcccacaa
     6481 aagtgactca agtcgtgaag tgccagaatt actcaagttt acgtgtaatg cgacccatga
     6541 gttggttgtt agaacacctc gtagtgtccg ccgtttgtct cgtaccatta agggtgtcga
     6601 atattttgaa gttattactt ttgagatggg ccaaaagaaa gcccccgacg gtagaattgt
     6661 tgagcttgtc aaggaagttt caaagagcta cccaatatct gaggggcctg agagagccaa
     6721 cgaattagta gaatcctata gaaaggcttc aaataaagct tattttgagt ggactattga
     6781 ggccagagat ctttctctgt tgggttccca tgttcgtaaa gctacctacc agacttacgc
     6841 tccaattctt tatgagaatg accacttttt cgactacatg caaaaaagta agtttcatct
     6901 caccattgaa ggtccaaaag tacttgctta tttacttggt ttatggattg gtgatggatt
     6961 gtctgacagg gcaacttttt cggttgattc cagagatact tctttgatgg aacgtgttac
     7021 tgaatatgct gaaaagttga atttgtgcgc cgagtataag gacagaaaag aaccacaagt
     7081 tgccaaaact gttaatttgt actctaaagt tgtcagaggt gctagcacaa atcctggttg
     7141 ttgtacagaa tgctggtcat caccatcatc accatatgag cctggctgag ttgaccctgg
     7201 agcctgtgca ccgccgaccc gagctcctgg atgcttgtgc tgacctcatc aatgatcagt
     7261 ggccccgcag ccgcacctcc cgcctgcact ccctgggcca gtcctcagat gccttccccc
     7321 tctgcctgat gctgctaagc ccccacccca cacttgaagc agcacccgtt gtggtgggcc
     7381 atgcccgcct gtcacgggtg ctgaaccagc cccagagcct cttagtggag acagtggtgg
     7441 tggcccgggc cctgaggggc cgtggctttg gccgccgcct catggagggc ctggaggtct
     7501 ttgctcgggc ccggggcttc cgcaagctgc atctcaccac ccatgaccag gtgcacttct
     7561 atacccacct gggctaccag ctgggtgagc ctgtgcaggg cctggtcttc accagcagac
     7621 ggctgcctgc caccctgctt aatgccttcc ccacagcccc ctctccccgg ccacccagga
     7681 aggccccaaa cctgactgcc caagctgccc caaggggtcc caagggacct ccattgccac
     7741 caccccctcc cctacctgag tgcctgacca tctcaccccc agttccatca gggccccctt
     7801 caaaaagcct gctggagaca caatatcaaa atgtgagggg gcgccccata ttctggatgg
     7861 aaaaagacat ctgactcgag cccgggtgac tgcaggaagg ggatccggct gctaacaaag
     7921 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     7981 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc
     8041 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac
     8101 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
     8161 gcaatttaac tgtgataaac taccgcatta aagcttggca ctggccgtcg ttttacaacg
     8221 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     8281 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     8341 cctgaatggc gaatggcagc ttggctgttt tggcggatga gataagattt tcagcctgat
     8401 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
     8461 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
     8521 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
     8581 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     8641 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
     8701 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
     8761 atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg
     8821 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8881 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8941 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9001 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9061 gaagaacgtt tcccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9121 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9181 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9241 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9301 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9361 gatcgttgcg atccggagct gaatgaagcc ataccaaacg acttctgcgc tmggcccttc
     9421 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9481 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9541 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9601 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaccccggt
     9661 tgataatcag aaaagcccca aaaacaggaa gattgtataa gcaaatattt aaattgtaaa
     9721 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     9781 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     9841 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     9901 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt
     9961 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
    10021 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
    10081 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
    10141 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcaaa taagcgttga
    10201 tattcagtca attacaaaca ttaataacga agagatgaca gaaaaatttt cattctgtga
    10261 cagagaaaaa gtagccgaag atgacggttt gtcacatgga gttggcagga tgtttgatta
    10321 aaaacataac
//
