LOCUS       sequence-37312-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, 
            Cuervo AM, Verkhusha VV
  TITLE     Monomeric fluorescent timers that change color from blue to red 
            report on cellular trafficking.
  JOURNAL   Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.
  PUBMED    19136976
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-02"; volume: "5(2)"; pages: "118-26. Epub 2009 
            Jan 11"
COMMENT     Sequence Label: pTRE-Medium-FT
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..761
                     /codon_start=1
                     /product="blue-to-red fluorescent timer derivative of 
                     mCherry (Subach et al., 2009)"
                     /label=Medium-FT
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHLEGSVDGHEFEIEGEGEGRPYEG
                     TQSAKLKVTKGGPLPFAWDILSPQFMYGSRAYVKHPADIPDYWKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERIYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVLLPGAYNVNIKMDITSHNEDYTIVEQCERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(196..214)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     464..483
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(793..812)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     847..866
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgactcatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc atggtgagca
       61 agggcgagga ggataacatg gccatcatca aggaattcat gcgtttcaag gtgcacctgg
      121 agggctccgt ggacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      181 agggcaccca gagcgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      241 acatcctgtc ccctcagttc atgtacggct ccagggccta cgtgaagcac cccgccgaca
      301 tccccgacta ctggaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      361 tcgaggatgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      421 tctacaaggt gaagctgcgc ggcaccaact tcccttccga cggccccgta atgcagaaga
      481 agaccatggg ctgggaggcc tcctccgagc ggatataccc cgaggacggc gccctgaagg
      541 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
      601 ccacctacaa ggccaagaag cccgtgctgc tgcccggcgc ctacaacgtc aacatcaaga
      661 tggacatcac ctcccacaac gaggactaca ccatcgttga acagtgcgaa cgcgccgagg
      721 gccaccattc caccggcggc atggacgagc tgtacaagta atctagagga tccagacatg
      781 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      841 atttgtgaaa tttgtgatgc tattgc
//
