LOCUS       sequence-373128-       10945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GAKIN in mammalian cells with Ha tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Lamason RL, Kupfer A, Pomerantz JL
  TITLE     The dynamic distribution of CARD11 at the immunological synapse is 
            regulated by the inhibitory kinesin GAKIN.
  JOURNAL   Mol Cell. 2010 Dec 10;40(5):798-809. doi: 
            10.1016/j.molcel.2010.11.007.
  PUBMED    21145487
REFERENCE   2  (bases 1 to 10945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2010.11"; journalName: "Mol Cell"; date: 
            "2010-12-10- 10"; volume: "40"; issue: "5"; pages: "798-809"
COMMENT     Sequence Label: pc-GAKIN-Ha
FEATURES             Location/Qualifiers
     source          1..10945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1701..1710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6221..6247
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(6264..6282)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6265..6282)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6302..6319)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6308..6532
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6578..7006
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6665..6684)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6875..6896
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7020..7349
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7200..7335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7262..7281
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7416..8210
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7470..7489)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8080..8099
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8384..8505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8421..8440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8475..8494
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8554..8570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8554..8570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8567..8589)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8578..8594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8602..8608,8609..8626,8627..8632))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8647..8668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8785..8802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8956..9544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9036..9055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9715..10506,10507..10575))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10338..10357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10576..10680)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10755..10774)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgggc cccccctcga ggtcgaccca cgcgtccgag ccgaacgagt tcctcggcgg
      721 ctgcaggatg ggggactcca aagtgaaagt ggcggtgcgg atacgaccca tgaaccggcg
      781 agagactgac ttgcatacca aatgtgtggt ggatgtggat gcaaacaagg ttattcttaa
      841 tcctgtaaat acgaatcttt ccaaaggaga tgcccggggc cagccgaagg tgtttgctta
      901 tgatcattgt ttctggtcta tggatgaatc tgtcaaagaa aagtatgcag gtcaagatat
      961 tgttttcaag tgccttggag agaatatcct gcagaatgct tttgatggct acaatgcatg
     1021 tatctttgcc tatggacaga ctggctctgg aaaatcttat accatgatgg gcacagctga
     1081 ccaacctgga ttaatcccaa gactttgcag tggactcttt gaacgaactc agaaagagga
     1141 aaatgaagaa cagagtttta aagtagaagt gtcctacatg gaaatttata atgaaaaagt
     1201 tcgagacctt cttgatccca aaggaagccg tcagacgttg aaagtcagag agcatagtgt
     1261 gttgggacct tatgtcgacg gactttctaa actggctgtc acaagctaca aggatattga
     1321 gtcgttgatg tctgagggta acaaatctcg cacagttgct gcaaccaaca tgaacgagga
     1381 gagtagccga tcccatgcag ttttcaaaat caccctcaca catactctct acgatgtgaa
     1441 gtctgggaca tctggagaga aagtgggcaa actcagcctg gtggatttag ctggcagtga
     1501 acgagcaacg aagacaggcg ctgcagggga caggctgaag gaagggagca acattaacaa
     1561 gtccctcaca accctcggtc tggttatctc agctcttgca gatcagagtg ctggcaaaaa
     1621 caagaataaa tttgttccat atcgtgactc agttctcact tggctgctca aagacagcct
     1681 cgggggtaac agcaagaccg ccatggtggc tactgtgagt cctgcagctg ataactatga
     1741 tgaaaccctc tcaactctgc ggtatgcaga tcgagccaag cacattgtaa accacgctgt
     1801 ggtgaatgag gaccctaatg cccgaattat ccgggatctc cgggaagaag ttgagaaact
     1861 ccgggagcag ctgaccaaag cagaggcaat gaaatctcca gagctaaagg accggctgga
     1921 agaatctgag aagctaatcc aggaaatgac tgtgacctgg gaggagaaat taaggaaaac
     1981 ggaggagatt gcacaggaac gacagaaaca gcttgagagt cttggaatat ctcttcagtc
     2041 ttcgggaatc aaagttgggg atgataaatg cttccttgtg aatctgaatg ctgacccagc
     2101 tctgaatgag cttctggtgt actatttaaa ggaacataca ttgatagggt cagcaaattc
     2161 ccaagatatc caactgtgcg gcatgggaat tcttcctgaa cactgtatta tagacatcac
     2221 gtcagaaggc caggttatgc tgactcctca gaagaacacc agaacatttg taaatgggtc
     2281 atctgtctcc agtccaatac agctacacca tggggacagg atattatggg gaaacaatca
     2341 tttcttcaga ctcaatttgc ctaaaaagaa aaagaaagca gaacgagagg atgaggacca
     2401 ggatccctcc atgaagaacg agaatagttc tgagcagctg gatgtagacg gagactcctc
     2461 cagcgaggtg tccagtgaag ttaactttaa ttacgaatac gcacagatgg aggtcaccat
     2521 gaaggccctg ggcagcaatg atccgatgca gtccatatta aacagcctag aacaacagca
     2581 tgaagaagaa aaacgatctg cactggagcg ccagaggctt atgtatgagc acgaattgga
     2641 gcagctccgg agaaggctgt ctcctgagaa gcagaactgc cggagcatgg acaggttttc
     2701 tttccactcg cccagcgctc agcaacgctt aagacagtgg gctgaggaga gagaagcaac
     2761 gttgaataac agcctgatga ggctgaggga acaaattgtt aaggccaatc tattggtgag
     2821 agaagctaat tacattgctg aggagctgga taaaagaaca gaatacaaag ttaccctaca
     2881 gattccagcc tccagcctgg atgccaacag gaagcgaggc tctcttctta gtgagcctgc
     2941 aatccaggtg agaagaaaag gaaaaggaaa gcagatttgg tctttggaaa aactggacaa
     3001 caggctgttg gatatgagag acctttatca ggagtggaaa gagtgtgaag aagataaccc
     3061 agtaatacga tcatacttca aacgtgctga tccattctat gatgagcagg aaaatcacag
     3121 tctcattggg gtggccaatg tcttcctcga gtcacttttc tatgatgtga agttacaata
     3181 cgctgttccc atcatcaacc agaaaggaga ggtggcaggt cggctgcacg tggaggtgat
     3241 gcgactcagt ggtgatgttg gggagaggat cgcaggaggc gatgaggtgg cagaggtctc
     3301 ctttgagaag gagacccagg agaacaaact ggtgtgcatg gttaaaatcc tgcaagctac
     3361 tgggttgcca cagcatctgt cccactttgt gttctgcaaa tacagcttct gggatcaaca
     3421 ggagccggtg attgtcgctc ctgaagtgga cacctcctcc tcttccgtca gcaaggagcc
     3481 gcactgcatg gttgtctttg atcattgcaa tgagttttct gttaacatca ccgaagactt
     3541 tatcgagcat ctttccgaag gagcattggc aattgaagta tatggacata aaataaacga
     3601 tccccggaaa aaccccgccc tgtgggattt gggaatcatc caagcaaaga cacgtagtct
     3661 tcgggacaga tggagtgaag tgaccaggaa attggaattc tgggttcaaa tcttggaaca
     3721 gaatgagaat ggtgaatact gccctgtaga agtgatttct gcaaaggatg tcccaacagg
     3781 aggaatcttc cagctccggc aggggcagtc ccggagagtt caagtcgaag tgaagtcagt
     3841 gcaggaatct gggactttac cactgatgga agaatgtata ctgtctgttg gcattggatg
     3901 tgtcaaagtt agaccgctca gagcccccag aacacatgag accttccatg aggaagagga
     3961 agacatggac agctaccagg atcgagattt agagagactt cgtagaaaat ggctaaatgc
     4021 attaacaaaa cgtcaggagt acttggatca acaattgcaa aagcttgtca gtaaacgtga
     4081 taaaacagag gatgatgctg accgtgaagc gcagcttctg gagatgcggt tgaccctaac
     4141 tgaggagagg aacgcggtga tggtcccctc tgctggcagt ggtattccag gggccccagc
     4201 agaatggacc ccagtacctg ggatggagac acacattcct gttatattcc tggacttaaa
     4261 tgctgatgat ttcagctctc aggataatct tgatgaccca gaagctggtg gatgggatgc
     4321 gaccttgact ggggaagaag aagaggagtt ctttgaattg cagattgtga agcagcatga
     4381 tggggaggtg aaagcagaag cctcctggga ctccgcggtg catggctgcc ctcagctcag
     4441 caggggcacg cccgtggacg agcggttgtt cctgatcgtg cgcgtgacgg tccagctcag
     4501 ccaccctgct gacatgcaac tggtgttacg caagagaatc tgtgtcaatg ttcacggccg
     4561 ccagggtttt gcacagagtc tcctaaaaaa gatgtctcat cgaagttcta ttcctggctg
     4621 tggagtgact tttgaaattg tctccaatat tccagaggat gcccagggag tggaagaacg
     4681 ggaagcatta gcaagaatgg cagccaatgt tgaaaaccca gcttctgctg actcggaggc
     4741 ttatattgaa aagtacctca ggagcgtgct ggctgtagaa aacctcctga ctttagatcg
     4801 tctgcgccag gaagttgcag tgaaggaaca gttaacagga aaaggaaagt tgagcaggag
     4861 gagtatcagt tctccaaatg tgaacagatt gtctggaagc cgacaagatc tcattccatc
     4921 atacagtcta ggcagcaaca agggccggtg ggaaagtcag caggatgtat cccaaaccac
     4981 agtttccaga ggaatagctc ctgcccccgc cctctctgtt tctccccaaa ataaccattc
     5041 tccagatcca ggactcagta accttgcagc atcctacttg aatcctgtca aatccttcgt
     5101 gccgcaaatg ccaaagctcc tcaagtctct ctttcccgtc cgcgatgaga agaggggcaa
     5161 gcggccgtct cccctcgcac accagcccgt gccccgcatc atggtgcagt cagccagccc
     5221 ggacatcagg gtgaccagga tggaggaggc tcagccggag atgggccctg acgtgctggt
     5281 gcagacgatg ggggccccgg ccttgaagat ctgcgacaaa cctgccaaag tgccttcccc
     5341 accgcctgtc atagctgtca cagcggtcac cccggctccg gaggcacagg acgggccccc
     5401 cagccccctg agtgaagcct ctagcgggta cttctcccac agcgtctcca ccgcgaccct
     5461 gtcggacgcc ctgggccccg gcctggacgc tgcggccccg ccggggtcca tgcccaccgc
     5521 ccctgaggcc gagcccgagg cgcccatcag ccacccccca ccgcccacgg ccgtccccgc
     5581 cgaggagccc cctggccccc agcagctcgt gagccccggt cgggagcgcc ccgacctcga
     5641 ggccccggcg cccggctccc cgttccgcgt ccggagggtg cgggcctcgg agttgcgctc
     5701 cttctcgcgc atgctggctg gggaccccgg ctgctccccg ggggccgagg ggaatgcgcc
     5761 ggccccgggc gccgggggac aggccctggc ctctgattcc gaggaagctg acgaggtccc
     5821 ggagtggctc cgagagggcg agttcgtcac cgtgggcgcc cacaaaacgg gcgtggtgag
     5881 atacgtgggg cctgccgact tccaagaggg cacgtgggtc ggcgtggagc tcgacctgcc
     5941 ctcaggtaag aatgacggtt ccatcggcgg gaagcagtac ttcaggtgta accctggcta
     6001 cgggctgctg gtcaggccca gccgggtccg cagggccacg ggccctgtgc ggcggcgcag
     6061 cacaggactc cggctgggtg cccccgaggc ccgccggagc gccaccctct cgggctccgc
     6121 caccaacctg gcctcgctga cagctgccct ggccaaggcc gacaggagcc acaagaaccc
     6181 tgagaaccgg aaatcctggg ccagcaggcg gccgctcgag tacccatacg acgtcccaga
     6241 ttacgctctc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
     6301 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6361 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6421 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6481 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6541 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6601 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6661 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6721 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6781 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6841 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6901 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6961 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7021 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7081 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7141 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7201 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7261 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7321 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7381 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     7441 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     7501 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     7561 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7621 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7681 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7741 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7801 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7861 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7921 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     7981 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     8041 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     8101 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     8161 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     8221 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     8281 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8341 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     8521 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8581 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8641 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8701 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8761 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8821 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8881 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8941 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9001 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9061 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9121 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9181 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9241 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9301 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9361 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9421 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9481 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9541 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9601 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9661 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9721 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9781 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9841 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9901 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9961 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10021 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10081 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10141 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10201 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10261 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10321 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10381 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10441 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10501 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10561 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10621 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10681 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
    10741 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
    10801 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
    10861 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
    10921 gatgtacggg ccagatatac gcgtt
//
