LOCUS       sequence-373134-        1659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for optogenetic long-term silencing of neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Rodriguez-Rozada S, Wietek J, Tenedini F, Sauter K, Dhiman N, 
            Hegemann P, Soba P, Wiegert JS
  TITLE     Aion is a bistable anion-conducting channelrhodopsin that provides 
            temporally extended and reversible neuronal silencing.
  JOURNAL   Commun Biol. 2022 Jul 9;5(1):687. doi: 10.1038/s42003-022-03636-x.
  PUBMED    35810216
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-07-9"; volume: "5(1):687. doi"; pages: " 
            10.1038/s42003-022-03636-x"
COMMENT     Sequence Label: hSyn-Aion-citrine
FEATURES             Location/Qualifiers
     source          1..1659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(940..942,943..945,946..1656)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(985..1006)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1246..1265)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1593..1614
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggactatg gcggcgcttt gtctgccgtc ggactcttcc aaacctcata cactcttgag
       61 aacaatggtt ctgttatttg catcccgaac aacggccagt gcttctgctt ggcttggctt
      121 aaatccaacg gaacaaatgc cgagaagttg gctgccaaca ttctgcagtg gatttctttt
      181 gcgctttcag cgctctgcct gatgttctac ggctaccaga cctggaagtc tacttgcggc
      241 tgggagaaca tttacgtggc cacgatccag atgatcaagt tcatcatcga gtatttccat
      301 agctttgacg aacctgcggt gatctactca tccaacggca acaagacccg gtggcttcgt
      361 tacgcgagct ggctgctgac cgcccctgtc attcttatcc acctgagcaa ccttacgggt
      421 ctggcgaacg actataacaa gcgtaccatg ggtctgctgg tgtcatgcat cggcggcatc
      481 gtgtgggcca ccacggccgc gctgtccaag ggatacgtcc gtgtcatttt cttcctgatg
      541 ggcctgtgct acggcatcta cacattcttc aacgcagcca aggtctacat tgaggcgtac
      601 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
      661 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
      721 agccggtacg gctccaacgt cggccacacc atcattgacc tgatgtcgaa gcagtgctgg
      781 ggtctgctcg gccactacct gcgcgtgctg atccacagcc atatcctcat ccacggcgac
      841 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
      901 gaggacgagg ccgaggctgg cgcggtcgcg gcactcgaga tggtgagcaa gggcgaggag
      961 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1021 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1081 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
     1141 ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1201 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1261 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1321 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1381 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1441 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1501 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
     1561 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1621 gccgggatca ctctcggcat ggacgagctg tacaagtag
//
