LOCUS       sequence-373136-        1659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for optogenetic long-term silencing of Cre-positive 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Ef1a-DIO-Aion-citrine
FEATURES             Location/Qualifiers
     source          1..1659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(4..714,715..717,718..720))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     395..414
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
       61 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt agctcaggta
      121 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      181 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
      241 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
      301 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      361 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      421 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      481 cttcatgtgg tcggggtagc gggcgaagca catcaggccg tagccgaagg tggtcacgag
      541 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
      601 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
      661 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
      721 ctcgagtgcc gcgaccgcgc cagcctcggc ctcgtcctcc accagcgtct cgacctcaat
      781 ctcagtgcca ccaatgttca atttggtggt cttgcgaatg tcgccgtgga tgaggatatg
      841 gctgtggatc agcacgcgca ggtagtggcc gagcagaccc cagcactgct tcgacatcag
      901 gtcaatgatg gtgtggccga cgttggagcc gtaccggctc aggacgccga agccctcggg
      961 gccgaggatg aacaggatgg ggaacatacc ccatgatacg aagaagagcc aagccatgcc
     1021 agtcaccacc tggcgacacc ggcccttcgg cacggtgtgg tacgcctcaa tgtagacctt
     1081 ggctgcgttg aagaatgtgt agatgccgta gcacaggccc atcaggaaga aaatgacacg
     1141 gacgtatccc ttggacagcg cggccgtggt ggcccacacg atgccgccga tgcatgacac
     1201 cagcagaccc atggtacgct tgttatagtc gttcgccaga cccgtaaggt tgctcaggtg
     1261 gataagaatg acaggggcgg tcagcagcca gctcgcgtaa cgaagccacc gggtcttgtt
     1321 gccgttggat gagtagatca ccgcaggttc gtcaaagcta tggaaatact cgatgatgaa
     1381 cttgatcatc tggatcgtgg ccacgtaaat gttctcccag ccgcaagtag acttccaggt
     1441 ctggtagccg tagaacatca ggcagagcgc tgaaagcgca aaagaaatcc actgcagaat
     1501 gttggcagcc aacttctcgg catttgttcc gttggattta agccaagcca agcagaagca
     1561 ctggccgttg ttcgggatgc aaataacaga accattgttc tcaagagtgt atgaggtttg
     1621 gaagagtccg acggcagaca aagcgccgcc atagtccat
//
