LOCUS       sequence-373138-        1854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for optogenetic long-term silencing of Cre-positive 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Ef1a-DIO-somAion-citrine
FEATURES             Location/Qualifiers
     source          1..1854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(199..909,910..912))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     590..609
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcagaagcgg gtggcctcgg ggaagtcggt ggcgcagctg atgaagctgt cgatgctgct
       61 catgctgcgc atgtcgatca cgccctcggt gcgggcgggc aggggggcca cggggctggg
      121 catgctgctc atctccagct cctcgggggg cttgctctgg ggggccatct ccttggtgtt
      181 caggatgggc tggctctgct tgtacagctc gtccatgccc agggtgatgc cggcggcggt
      241 cacgaactcc agcagcacca tgtggtcgcg cttctcgttg gggtccttgc tcagggcgct
      301 ctggtagctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
      361 ctgctggtag tggtcggcca gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
      421 gttcaccttg atgccgttct tctgcttgtc ggccatgatg tacacgttgt ggctgttgta
      481 gttgtactcc agcttgtggc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
      541 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
      601 gccgtcgtcc ttgaagaaga tggtgcgctc ctgcacgtag ccctcgggca tggcgctctt
      661 gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcacatca ggccgtagcc
      721 gaaggtggtc accagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
      781 cagggtcagc ttgccgtagg tggcgtcgcc ctcgccctcg ccgctcacgc tgaacttgtg
      841 gccgttcacg tcgccgtcca gctccaccag gatgggcacc acgccggtga acagctcctc
      901 gcccttgctc accacctcga gtgccgcgac cgcgccagcc tcggcctcgt cctccaccag
      961 cgtctcgacc tcaatctcag tgccaccaat gttcaatttg gtggtcttgc gaatgtcgcc
     1021 gtggatgagg atatggctgt ggatcagcac gcgcaggtag tggccgagca gaccccagca
     1081 ctgcttcgac atcaggtcaa tgatggtgtg gccgacgttg gagccgtacc ggctcaggac
     1141 gccgaagccc tcggggccga ggatgaacag gatggggaac ataccccatg atacgaagaa
     1201 gagccaagcc atgccagtca ccacctggcg acaccggccc ttcggcacgg tgtggtacgc
     1261 ctcaatgtag accttggctg cgttgaagaa tgtgtagatg ccgtagcaca ggcccatcag
     1321 gaagaaaatg acacggacgt atcccttgga cagcgcggcc gtggtggccc acacgatgcc
     1381 gccgatgcat gacaccagca gacccatggt acgcttgtta tagtcgttcg ccagacccgt
     1441 aaggttgctc aggtggataa gaatgacagg ggcggtcagc agccagctcg cgtaacgaag
     1501 ccaccgggtc ttgttgccgt tggatgagta gatcaccgca ggttcgtcaa agctatggaa
     1561 atactcgatg atgaacttga tcatctggat cgtggccacg taaatgttct cccagccgca
     1621 agtagacttc caggtctggt agccgtagaa catcaggcag agcgctgaaa gcgcaaaaga
     1681 aatccactgc agaatgttgg cagccaactt ctcggcattt gttccgttgg atttaagcca
     1741 agccaagcag aagcactggc cgttgttcgg gatgcaaata acagaaccat tgttctcaag
     1801 agtgtatgag gtttggaaga gtccgacggc agacaaagcg ccgccatagt ccat
//
