LOCUS       sequence-373139-        1638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for optogenetic long-term silencing of neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hSyn-Aion-mKate2
FEATURES             Location/Qualifiers
     source          1..1638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             940..1638
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 atggactatg gcggcgcttt gtctgccgtc ggactcttcc aaacctcata cactcttgag
       61 aacaatggtt ctgttatttg catcccgaac aacggccagt gcttctgctt ggcttggctt
      121 aaatccaacg gaacaaatgc cgagaagttg gctgccaaca ttctgcagtg gatttctttt
      181 gcgctttcag cgctctgcct gatgttctac ggctaccaga cctggaagtc tacttgcggc
      241 tgggagaaca tttacgtggc cacgatccag atgatcaagt tcatcatcga gtatttccat
      301 agctttgacg aacctgcggt gatctactca tccaacggca acaagacccg gtggcttcgt
      361 tacgcgagct ggctgctgac cgcccctgtc attcttatcc acctgagcaa ccttacgggt
      421 ctggcgaacg actataacaa gcgtaccatg ggtctgctgg tgtcatgcat cggcggcatc
      481 gtgtgggcca ccacggccgc gctgtccaag ggatacgtcc gtgtcatttt cttcctgatg
      541 ggcctgtgct acggcatcta cacattcttc aacgcagcca aggtctacat tgaggcgtac
      601 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
      661 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
      721 agccggtacg gctccaacgt cggccacacc atcattgacc tgatgtcgaa gcagtgctgg
      781 ggtctgctcg gccactacct gcgcgtgctg atccacagcc atatcctcat ccacggcgac
      841 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
      901 gaggacgagg ccgaggctgg cgcggtcgcg gcactcgaga tggtgagcga gctgattaag
      961 gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc
     1021 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggcggtc
     1081 gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc
     1141 aaaaccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccccgag
     1201 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     1261 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     1321 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctc caccgagacc
     1381 ctgtaccccg ctgacggcgg cctggaaggc agagccgaca tggccctgaa gctcgtgggc
     1441 gggggccacc tgatctgcaa cttgaagacc acatacagat ccaagaaacc cgctaagaac
     1501 ctcaagatgc ccggcgtcta ctatgtggac agaagactgg aaagaatcaa ggaggccgac
     1561 aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc
     1621 aaactggggc acagatga
//
