LOCUS       sequence-373141-        1743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for optogenetic long-term silencing of excitatory 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CaMKII-Aion-ts-citrine-ER
FEATURES             Location/Qualifiers
     source          1..1743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1003..1005,1006..1008,1009..1719)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1048..1069)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1309..1328)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1656..1677
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggactatg gcggcgcttt gtctgccgtc ggactcttcc aaacctcata cactcttgag
       61 aacaatggtt ctgttatttg catcccgaac aacggccagt gcttctgctt ggcttggctt
      121 aaatccaacg gaacaaatgc cgagaagttg gctgccaaca ttctgcagtg gatttctttt
      181 gcgctttcag cgctctgcct gatgttctac ggctaccaga cctggaagtc tacttgcggc
      241 tgggagaaca tttacgtggc cacgatccag atgatcaagt tcatcatcga gtatttccat
      301 agctttgacg aacctgcggt gatctactca tccaacggca acaagacccg gtggcttcgt
      361 tacgcgagct ggctgctgac cgcccctgtc attcttatcc acctgagcaa ccttacgggt
      421 ctggcgaacg actataacaa gcgtaccatg ggtctgctgg tgtcatgcat cggcggcatc
      481 gtgtgggcca ccacggccgc gctgtccaag ggatacgtcc gtgtcatttt cttcctgatg
      541 ggcctgtgct acggcatcta cacattcttc aacgcagcca aggtctacat tgaggcgtac
      601 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
      661 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
      721 agccggtacg gctccaacgt cggccacacc atcattgacc tgatgtcgaa gcagtgctgg
      781 ggtctgctcg gccactacct gcgcgtgctg atccacagcc atatcctcat ccacggcgac
      841 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
      901 gaggacgagg ccgaggctgg cgcggtcgcg gcactcgaga agagcaggat caccagcgag
      961 ggcgagtaca tccccctgga ccagatcgac atcaacgtgg tgatggtgag caagggcgag
     1021 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1081 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1141 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
     1201 tacggcctga tgtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     1261 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1321 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1381 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1441 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1501 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1561 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     1621 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1681 gccgccggga tcactctcgg catggacgag ctgtacaagt tctgctacga gaacgaggtg
     1741 taa
//
