LOCUS       sequence-373142-        1722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for optogenetic long-term silencing of neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hSyn-Aion-ts-mScarlet-ER
FEATURES             Location/Qualifiers
     source          1..1722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1003..1698
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1136..1154)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1404..1423
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggactatg gcggcgcttt gtctgccgtc ggactcttcc aaacctcata cactcttgag
       61 aacaatggtt ctgttatttg catcccgaac aacggccagt gcttctgctt ggcttggctt
      121 aaatccaacg gaacaaatgc cgagaagttg gctgccaaca ttctgcagtg gatttctttt
      181 gcgctttcag cgctctgcct gatgttctac ggctaccaga cctggaagtc tacttgcggc
      241 tgggagaaca tttacgtggc cacgatccag atgatcaagt tcatcatcga gtatttccat
      301 agctttgacg aacctgcggt gatctactca tccaacggca acaagacccg gtggcttcgt
      361 tacgcgagct ggctgctgac cgcccctgtc attcttatcc acctgagcaa ccttacgggt
      421 ctggcgaacg actataacaa gcgtaccatg ggtctgctgg tgtcatgcat cggcggcatc
      481 gtgtgggcca ccacggccgc gctgtccaag ggatacgtcc gtgtcatttt cttcctgatg
      541 ggcctgtgct acggcatcta cacattcttc aacgcagcca aggtctacat tgaggcgtac
      601 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
      661 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
      721 agccggtacg gctccaacgt cggccacacc atcattgacc tgatgtcgaa gcagtgctgg
      781 ggtctgctcg gccactacct gcgcgtgctg atccacagcc atatcctcat ccacggcgac
      841 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
      901 gaggacgagg ccgaggctgg cgcggtcgcg gcactcgaga agagcaggat caccagcgag
      961 ggcgagtaca tccccctgga ccagatcgac atcaacgtgg tgatggtgag caagggcgag
     1021 gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat gaacggccac
     1081 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     1141 ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc ccctcagttc
     1201 atgtacggct ccagggcctt caccaagcac cccgccgaca tccccgacta ctataagcag
     1261 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg
     1321 accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt gaagctccgc
     1381 ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg ctgggaagcg
     1441 tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa gatggccctg
     1501 cgcctgaagg acggcggacg ctacctggcg gacttcaaga ccacctacaa ggccaagaag
     1561 cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt tggacatcac ctcccacaac
     1621 gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc caccggcggc
     1681 atggacgagc tgtacaagtt ctgctacgag aacgaggtgt aa
//
