LOCUS       sequence-373144-        3261 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for bidirectional optogenetic manipulation of 
            tTA-positive neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TRE3GV-somBiPOLES-mKate2
FEATURES             Location/Qualifiers
     source          1..3261
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             958..1653
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             2014..3057
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
ORIGIN
        1 atggcatcac aggtcgtcta tggggaatgg gcatcaactc acaccgagtg ctacaatatg
       61 tccagaatcg actccacctt cgtctcactg ctgcagctgg tgtgggccgt ggtctcaggc
      121 tgccagacaa tcttcatgat tagcagggct cccaaggtcc cttgggagtc cgtgtacctg
      181 ccctttgtcg aatctatcac ttatgcactg gccagtactg gcaacggaac cctgcagatg
      241 agggacggca gattctttcc ttggagccga atggcctcct ggctgtgcac ctgtccaatc
      301 atgctgggac agatttctaa catggctctg gtgaagtaca aaagtatccc actgaatccc
      361 attgcacagg ccgcttccat cattagagtg gtcatgggaa tcaccgctac aatttctccc
      421 gcagagtata tgaagtggct gttctttttc tttggggcca cctgcctggt gttcgagtac
      481 agtgtggtct tcacaatctt tcaagtgggg ctgtatggct ttgaatctgt cgggacacct
      541 ctggcacaga aggtggtggt gaggatcaag atgctgcgcc tgatcttctt tattgcttgg
      601 actatgttcc caatcgtgtg gctgatttca cccaccggag tctgcgtgat ccacgagaac
      661 gtgagcgcca ttctgtacct gctggctgac ggcctgtgca aaaatacata tggagtgatc
      721 ctgtggagca ctgcctgggg agtgctggaa gggaagtggg accccgcatg tctgccaggg
      781 caggagaaac ccgaagccga cgatcctttt ggcctgaatc acgagaagaa tgcccctcct
      841 aacgacgaag tcaatatcag aatgtttgga aggggacgta cggcggccaa gagcaggatc
      901 accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtggc ggtacccatg
      961 gtgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
     1021 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     1081 atgagaatca aggcggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
     1141 agcttcatgt acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt
     1201 aagcagtcct tccccgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     1261 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     1321 atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
     1381 gaggcctcca ccgagaccct gtaccccgct gacggcggcc tggaaggcag agccgacatg
     1441 gccctgaagc tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc
     1501 aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa
     1561 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga
     1621 tactgcgacc tccctagcaa actggggcac agaaccggtg cagccctgca ggagaagaag
     1681 tcatgcagcc agcgcatggc cgaattccgg caatactgtt ggaacccgga cactgggcag
     1741 atgctgggcc gcaccccagc ccggtgggtg tggatcagcc tgtactatgc agctttctac
     1801 gtggtcatga ctgggctctt tgccttgtgc atctatgtgc tgatgcagac cattgatccc
     1861 tacacccccg actaccagga ccagttaaag tcaccggggg taaccttgag accggatgtg
     1921 tatggggaaa gagggctgca gatttcctac aacatctctg aaaacagctc tagacaggcc
     1981 cagatcaccg gacgtccgga gggagctgta caagagctga tcagcagcgc caccagatct
     2041 ctgtttgccg ccggaggcat caacccttgg cctaacccct accaccacga ggacatgggc
     2101 tgtggaggaa tgacacctac aggcgagtgc ttcagcaccg agtggtggtg tgacccttct
     2161 tacggactga gcgacgccgg atacggatat tgcttcgtgg aggccacagg cggctacctg
     2221 gtcgtgggag tggagaagaa gcaggcttgg ctgcacagca gaggcacacc aggagaaaag
     2281 atcggcgccc aggtctgcca gtggattgct ttcagcatcg ccatcgccct gctgacattc
     2341 tacggcttca gcgcctggaa ggccacttgc ggttgggagg aggtctacgt ctgttgcgtc
     2401 gaggtgctgt tcgtgaccct ggagatcttc aaggagttca gcagccccgc cacagtgtac
     2461 ctgtctaccg gcaaccacgc ctattgcctg cgctacttcg agtggctgct gtcttgcccc
     2521 gtgatcctga tcaagctgag caacctgagc ggcctgaaga acgactacag caagcggacc
     2581 atgggcctga tcgtgtcttg cgtgggaatg atcgtgttcg gcatggccgc aggactggct
     2641 accgattggc tcaagtggct gctgtatatc gtgtcttgca tctacggcgg ctacatgtac
     2701 ttccaggccg ccaagtgcta cgtggaagcc aaccacagcg tgcctaaagg ccattgccgc
     2761 atggtcgtga agctgatggc ctacgcttac ttcgcctctt ggggcagcta cccaatcctc
     2821 tgggcagtgg gaccagaagg actgctgaag ctgagccctt acgccaacag catcggccac
     2881 agcatctgcg acatcatcgc caaggagttt tggaccttcc tggcccacca cctgaggatc
     2941 aagatccacg agcacatcct gatccacggc gacatccgga agaccaccaa gatggagatc
     3001 ggaggcgagg aggtggaagt ggaagagttc gtggaggagg aggacgagga cacagtgctc
     3061 gagcagagcc agcccatcct gaacaccaag gagatggccc cccagagcaa gccccccgag
     3121 gagctggaga tgagcagcat gcccagcccc gtggcccccc tgcccgcccg caccgagggc
     3181 gtgatcgaca tgcgcagcat gagcagcatc gacagcttca tcagctgcgc caccgacttc
     3241 cccgaggcca cccgcttctg a
//
