LOCUS       sequence-373145-        3213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for bidirectional optogenetic manipulation of neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Vierock J, Rodriguez-Rozada S, Dieter A, Pieper F, Sims R, Tenedini 
            F, Bergs ACF, Bendifallah I, Zhou F, Zeitzschel N, Ahlbeck J, 
            Augustin S, Sauter K, Papagiakoumou E, Gottschalk A, Soba P, 
            Emiliani V, Engel AK, Hegemann P, Wiegert JS
  TITLE     BiPOLES is an optogenetic tool developed for bidirectional 
            dual-color control of neurons.
  JOURNAL   Nat Commun. 2021 Jul 26;12(1):4527. doi: 10.1038/s41467-021-24759-5.
  PUBMED    34312384
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-26"; volume: "12(1):4527. doi"; pages: " 
            10.1038/s41467-021-24759-5"
COMMENT     Sequence Label: hSyn-GtACR2-ChRmine-tandem-eYFP
FEATURES             Location/Qualifiers
     source          1..3213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2302..2304,2305..3015)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2344..2365)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2605..2624)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2952..2973
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggcatcac aggtcgtcta tggggaatgg gcatcaactc acaccgagtg ctacaatatg
       61 tccagaatcg actccacctt cgtctcactg ctgcagctgg tgtgggccgt ggtctcaggc
      121 tgccagacaa tcttcatgat tagcagggct cccaaggtcc cttgggagtc cgtgtacctg
      181 ccctttgtcg aatctatcac ttatgcactg gccagtactg gcaacggaac cctgcagatg
      241 agggacggca gattctttcc ttggagccga atggcctcct ggctgtgcac ctgtccaatc
      301 atgctgggac agatttctaa catggctctg gtgaagtaca aaagtatccc actgaatccc
      361 attgcacagg ccgcttccat cattagagtg gtcatgggaa tcaccgctac aatttctccc
      421 gcagagtata tgaagtggct gttctttttc tttggggcca cctgcctggt gttcgagtac
      481 agtgtggtct tcacaatctt tcaagtgggg ctgtatggct ttgaatctgt cgggacacct
      541 ctggcacaga aggtggtggt gaggatcaag atgctgcgcc tgatcttctt tattgcttgg
      601 actatgttcc caatcgtgtg gctgatttca cccaccggag tctgcgtgat ccacgagaac
      661 gtgagcgcca ttctgtacct gctggctgac ggcctgtgca aaaatacata tggagtgatc
      721 ctgtggagca ctgcctgggg agtgctggaa gggaagtggg accccgcatg tctgccaggg
      781 caggagaaac ccgaagccga cgatcctttt ggcctgaatc acgagaagaa tgcccctcct
      841 aacgacgaag tcaatatcag aatgtttgga aggggacgta cggcggccaa gagcaggatc
      901 accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtggc ggtagcagcc
      961 ctgcaggaga agaagtcatg cagccagcgc atggccgaat tccggcaata ctgttggaac
     1021 ccggacactg ggcagatgct gggccgcacc ccagcccggt gggtgtggat cagcctgtac
     1081 tatgcagctt tctacgtggt catgactggg ctctttgcct tgtgcatcta tgtgctgatg
     1141 cagaccattg atccctacac ccccgactac caggaccagt taaagtcacc gggggtaacc
     1201 ttgagaccgg atgtgtatgg ggaaagaggg ctgcagattt cctacaacat ctctgaaaac
     1261 agctctagac aggcccagat caccggacgt ccggagggag ctgtaatggc acacgcacca
     1321 ggcaccgacc agatgttcta cgtgggcaca atggacggct ggtatctgga taccaagctg
     1381 aactccgtgg ccatcggcgc ccactggtct tgctttatcg tgctgacaat caccacattc
     1441 tacctgggct atgagtcctg gacatctcgg ggccctagca agagaacctc cttttacgcc
     1501 ggctatcagg aggagcagaa cctggccctg tttgtgaatt tctttgccat gctgagctac
     1561 ttcggcaaga tcgtggccga cacactgggc cacaacttcg gcgatgtggg ccccttcatc
     1621 atcggcttcg gcaattacag gtatgccgac tacatgctga cctgcccaat gctggtgtac
     1681 gatctgctgt atcagctgag ggccccctat cgcgtgtctt gtagcgccat catctttgcc
     1741 atcctgatgt ctggcgtgct ggccgagttc tacgcagagg gcgaccctag gctgaggaat
     1801 ggcgcctacg cctggtatgg ctttggctgt ttctggttta tcttcgccta ctctatcgtg
     1861 atgagcatcg tggccaagca gtatagccgg ctggcccagc tggcccagga tacaggcgcc
     1921 gagcacagcc tgcacgtgct gaagttcgcc gtgtttacct tctccatgct gtggattctg
     1981 tttcccctgg tgtgggccat ctgccctaga ggcttcggct ggatcgacga taactggaca
     2041 gaggtggccc actgcgtgtg cgacatcgtg gccaagtcct gttacggctt tgccctggcc
     2101 cggttcagaa agacctatga tgaggagctg tttcggctgc tggagcagct gggacacgac
     2161 gaggatgagt tccagaagct ggagctggat atgaggctga gcagcaatgg cgagcgcctg
     2221 cggagactgt ctgcggcagc gaagagcagg atcaccagcg agggcgagta catccccctg
     2281 gaccagatcg acatcaacgt ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2341 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2401 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2461 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc
     2521 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2581 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2641 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2701 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2761 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2821 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2881 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     2941 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3001 gacgagctgt acaagcagag ccagcccatc ctgaacacca aggagatggc cccccagagc
     3061 aagccccccg aggagctgga gatgagcagc atgcccagcc ccgtggcccc cctgcccgcc
     3121 cgcaccgagg gcgtgatcga catgcgcagc atgagcagca tcgacagctt catcagctgc
     3181 gccaccgact tccccgaggc cacccgcttc tga
//
