LOCUS       sequence-373147-        3204 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for bidirectional optogenetic manipulation of excitatory 
            neurons with blue shifted excitation spectrum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CaMKII-somBiPOLES-ChroME2s-mCerulean
FEATURES             Location/Qualifiers
     source          1..3204
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1000..1021)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1261..1280)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1608..1629
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggcatcac aggtcgtcta tggggaatgg gcatcaactc acaccgagtg ctacaatatg
       61 tccagaatcg actccacctt cgtctcactg ctgcagctgg tgtgggccgt ggtctcaggc
      121 tgccagacaa tcttcatgat tagcagggct cccaaggtcc cttgggagtc cgtgtacctg
      181 ccctttgtcg aatctatcac ttatgcactg gccagtactg gcaacggaac cctgcagatg
      241 agggacggca gattctttcc ttggagccga atggcctcct ggctgtgcac ctgtccaatc
      301 atgctgggac agatttctaa catggctctg gtgaagtaca aaagtatccc actgaatccc
      361 attgcacagg ccgcttccat cattagagtg gtcatgggaa tcaccgctac aatttctccc
      421 gcagagtata tgaagtggct gttctttttc tttggggcca cctgcctggt gttcgagtac
      481 agtgtggtct tcacaatctt tcaagtgggg ctgtatggct ttgaatctgt cgggacacct
      541 ctggcacaga aggtggtggt gaggatcaag atgctgcgcc tgatcttctt tattgcttgg
      601 actatgttcc caatcgtgtg gctgatttca cccaccggag tctgcgtgat ccacgagaac
      661 gtgagcgcca ttctgtacct gctggctgac ggcctgtgca aaaatacata tggagtgatc
      721 ctgtggagca ctgcctgggg agtgctggaa gggaagtggg accccgcatg tctgccaggg
      781 caggagaaac ccgaagccga cgatcctttt ggcctgaatc acgagaagaa tgcccctcct
      841 aacgacgaag tcaatatcag aatgtttgga aggggacgta cggcggccaa gagcaggatc
      901 accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtggc ggtacccgtg
      961 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1021 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1081 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1141 accaccctga gctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1201 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1261 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1321 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1381 gagtacaacg ccatccacgg caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1441 aaggccaact tcggcctcaa ctgcaacatc gaggacggca gcgtgcagct cgccgaccac
     1501 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1561 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1621 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtatac cggtgcagcc
     1681 ctgcaggaga agaagtcatg cagccagcgc atggccgaat tccggcaata ctgttggaac
     1741 ccggacactg ggcagatgct gggccgcacc ccagcccggt gggtgtggat cagcctgtac
     1801 tatgcagctt tctacgtggt catgactggg ctctttgcct tgtgcatcta tgtgctgatg
     1861 cagaccattg atccctacac ccccgactac caggaccagt taaagtcacc gggggtaacc
     1921 ttgagaccgg atgtgtatgg ggaaagaggg ctgcagattt cctacaacat ctctgaaaac
     1981 agctctagac aggcccagat caccggacgt ccggagggag ctgtacaaga aacagccgcc
     2041 acaatgaccc acgcctttat ctcagccgtg cctagcgccg aagccacaat tagaggcctg
     2101 ctgagcgccg cagcagtggt gacaccagca gcagacgctc acggagaaac ctctaacgcc
     2161 acaacagccg gagccgatca cggttgcttc ccccacatca accacggaac cgagctgcag
     2221 cacaagatcg cagtgggact ccagtggttc accgtgatcg tggctatcgt gcagctcatc
     2281 ttctacggtt ggcacagctt caaggccaca accggctggg aggaggtcta cgtctgcgtg
     2341 atcgagctcg tcaagtgctt catcgagctg ttccacgagg tcgacagccc agccacagtg
     2401 taccagacca acggaggagc cgtgatttgg ctgcggtaca gcgagtggct cctgacttgc
     2461 cccgtgatcc tgatccacct gagcaacctg accggactgc acgaagagta cagcaagcgg
     2521 accatgacca tcctggtgac cgacatcggc aacatcgtgt gggggatcac agccgccttt
     2581 acaaagggcc ccctgaagat cctgttcttc atgatcggcc tgttctacgg cgtgacttgc
     2641 ttcttccaga tcgccaaggt gtatatcgag agctaccaca ccctgcccaa aggcgtctgc
     2701 cggaagattt gcaagatcat ggcctacgtc ttcttctgct cttggctgat gttccccgtg
     2761 atgttcatcg ccggacacga gggactgggc ctgatcacac cttacaccag cggaatcggc
     2821 cacctgatcc tggatctgat cgccaagaac acttggggct tcctgggcca ccacctgaga
     2881 gtgaagatcc acgagcacat cctgatccac ggcgacatcc ggaagacaac caccatcaac
     2941 gtggccggcg agaacatgga gatcgagacc ttcgtcgacg aggaggagga gggaggagtg
     3001 ctcgagcaga gccagcccat cctgaacacc aaggagatgg ccccccagag caagcccccc
     3061 gaggagctgg agatgagcag catgcccagc cccgtggccc ccctgcccgc ccgcaccgag
     3121 ggcgtgatcg acatgcgcag catgagcagc atcgacagct tcatcagctg cgccaccgac
     3181 ttccccgagg ccacccgctt ctga
//
