LOCUS       sequence-373149-        3279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for bidirectional optogenetic manipulation of 
            Cre-positive neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hSyn-DIO-somBiPOLES-eGFP
FEATURES             Location/Qualifiers
     source          1..3279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(205..1248)
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
     CDS             complement(join(1609..2319,2320..2322))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(1609..2319,2320..2322))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1651..1672)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2000..2019
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2259..2280
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tcagaagcgg gtggcctcgg ggaagtcggt ggcgcagctg atgaagctgt cgatgctgct
       61 catgctgcgc atgtcgatca cgccctcggt gcgggcgggc aggggggcca cggggctggg
      121 catgctgctc atctccagct cctcgggggg cttgctctgg ggggccatct ccttggtgtt
      181 caggatgggc tggctctgct cgagcactgt gtcctcgtcc tcctcctcca cgaactcttc
      241 cacttccacc tcctcgcctc cgatctccat cttggtggtc ttccggatgt cgccgtggat
      301 caggatgtgc tcgtggatct tgatcctcag gtggtgggcc aggaaggtcc aaaactcctt
      361 ggcgatgatg tcgcagatgc tgtggccgat gctgttggcg taagggctca gcttcagcag
      421 tccttctggt cccactgccc agaggattgg gtagctgccc caagaggcga agtaagcgta
      481 ggccatcagc ttcacgacca tgcggcaatg gcctttaggc acgctgtggt tggcttccac
      541 gtagcacttg gcggcctgga agtacatgta gccgccgtag atgcaagaca cgatatacag
      601 cagccacttg agccaatcgg tagccagtcc tgcggccatg ccgaacacga tcattcccac
      661 gcaagacacg atcaggccca tggtccgctt gctgtagtcg ttcttcaggc cgctcaggtt
      721 gctcagcttg atcaggatca cggggcaaga cagcagccac tcgaagtagc gcaggcaata
      781 ggcgtggttg ccggtagaca ggtacactgt ggcggggctg ctgaactcct tgaagatctc
      841 cagggtcacg aacagcacct cgacgcaaca gacgtagacc tcctcccaac cgcaagtggc
      901 cttccaggcg ctgaagccgt agaatgtcag cagggcgatg gcgatgctga aagcaatcca
      961 ctggcagacc tgggcgccga tcttttctcc tggtgtgcct ctgctgtgca gccaagcctg
     1021 cttcttctcc actcccacga ccaggtagcc gcctgtggcc tccacgaagc aatatccgta
     1081 tccggcgtcg ctcagtccgt aagaagggtc acaccaccac tcggtgctga agcactcgcc
     1141 tgtaggtgtc attcctccac agcccatgtc ctcgtggtgg taggggttag gccaagggtt
     1201 gatgcctccg gcggcaaaca gagatctggt ggcgctgctg atcagctctt gtacagctcc
     1261 ctccggacgt ccggtgatct gggcctgtct agagctgttt tcagagatgt tgtaggaaat
     1321 ctgcagccct ctttccccat acacatccgg tctcaaggtt acccccggtg actttaactg
     1381 gtcctggtag tcgggggtgt agggatcaat ggtctgcatc agcacataga tgcacaaggc
     1441 aaagagccca gtcatgacca cgtagaaagc tgcatagtac aggctgatcc acacccaccg
     1501 ggctggggtg cggcccagca tctgcccagt gtccgggttc caacagtatt gccggaattc
     1561 ggccatgcgc tggctgcatg acttcttctc ctgcagggct gcaccggtct tgtacagctc
     1621 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
     1681 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag
     1741 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
     1801 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
     1861 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
     1921 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
     1981 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
     2041 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
     2101 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
     2161 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
     2221 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     2281 gatgggcacc accccggtga acagctcctc gcccttgctc acgggtaccg ccacgttgat
     2341 gtcgatctgg tccaggggga tgtactcgcc ctcgctggtg atcctgctct tggccgccgt
     2401 acgtcccctt ccaaacattc tgatattgac ttcgtcgtta ggaggggcat tcttctcgtg
     2461 attcaggcca aaaggatcgt cggcttcggg tttctcctgc cctggcagac atgcggggtc
     2521 ccacttccct tccagcactc cccaggcagt gctccacagg atcactccat atgtattttt
     2581 gcacaggccg tcagccagca ggtacagaat ggcgctcacg ttctcgtgga tcacgcagac
     2641 tccggtgggt gaaatcagcc acacgattgg gaacatagtc caagcaataa agaagatcag
     2701 gcgcagcatc ttgatcctca ccaccacctt ctgtgccaga ggtgtcccga cagattcaaa
     2761 gccatacagc cccacttgaa agattgtgaa gaccacactg tactcgaaca ccaggcaggt
     2821 ggccccaaag aaaaagaaca gccacttcat atactctgcg ggagaaattg tagcggtgat
     2881 tcccatgacc actctaatga tggaagcggc ctgtgcaatg ggattcagtg ggatactttt
     2941 gtacttcacc agagccatgt tagaaatctg tcccagcatg attggacagg tgcacagcca
     3001 ggaggccatt cggctccaag gaaagaatct gccgtccctc atctgcaggg ttccgttgcc
     3061 agtactggcc agtgcataag tgatagattc gacaaagggc aggtacacgg actcccaagg
     3121 gaccttggga gccctgctaa tcatgaagat tgtctggcag cctgagacca cggcccacac
     3181 cagctgcagc agtgagacga aggtggagtc gattctggac atattgtagc actcggtgtg
     3241 agttgatgcc cattccccat agacgacctg tgatgccat
//
