LOCUS       sequence-37315-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, 
            Cuervo AM, Verkhusha VV
  TITLE     Monomeric fluorescent timers that change color from blue to red 
            report on cellular trafficking.
  JOURNAL   Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.
  PUBMED    19136976
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-02"; volume: "5(2)"; pages: "118-26. Epub 2009 
            Jan 11"
COMMENT     Sequence Label: pBAD/HisB-Fast-FT
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(10..720)
                     /codon_start=1
                     /product="blue-to-red fluorescent timer derivative of 
                     mCherry (Subach et al., 2009)"
                     /label=Fast-FT
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHVEGSVNGHEFEIEGEGKGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSRAYVKHPADIPDYWKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERS
                     EGRHSTGGMDELYK"
     primer_bind     complement(288..307)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     557..575
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(join(739..753,754..762))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(766..798)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(802..819)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgaattct tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggagcg
       61 ttcgtactgt tccacgatgg tgtagtcctc gttgtgggat gtgatgtcca acttgatatt
      121 gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc
      181 agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc
      241 gccgtcctcg gggtacatcc gctcggtgga ggcctcccag cccatggtct tcttctgcat
      301 tacggggccg tcggagggga agttagttcc gcgcagcttc accttgtaga tgaactcgcc
      361 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
      421 gcgctcccac ttgaagccct cggggaagga cagcttccag tagtcgggga tgtcggcggg
      481 atgcttcacg taggccctgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa
      541 gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
      601 gcccttgccc tcgccctcga tctcgaactc gtggccgttc acggaaccct ccacgtgcac
      661 tttgaaacgc atgaactctt tgatgatggc catgttatcc tcctcgccct tgctcaccat
      721 agatctcgag ctcggatcct tatcgtcatc gtcgtacaga tcccgaccca tttgctgtcc
      781 accagtcatg ctagccatac catgatgatg atgatgatga g
//
