LOCUS       sequence-373150-        4550 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral vector for bidirectional optogenetic manipulation of 
            Cre-positive neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Ef1a-somBiPOLES-mCerulean-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..4550
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1000..1021)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1261..1280)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1608..1629
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2029..3072
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
     misc_feature    3399..3948
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3543..3560)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3770..3789
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3951..4550
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3951..3970)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4447..4467
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 atggcatcac aggtcgtcta tggggaatgg gcatcaactc acaccgagtg ctacaatatg
       61 tccagaatcg actccacctt cgtctcactg ctgcagctgg tgtgggccgt ggtctcaggc
      121 tgccagacaa tcttcatgat tagcagggct cccaaggtcc cttgggagtc cgtgtacctg
      181 ccctttgtcg aatctatcac ttatgcactg gccagtactg gcaacggaac cctgcagatg
      241 agggacggca gattctttcc ttggagccga atggcctcct ggctgtgcac ctgtccaatc
      301 atgctgggac agatttctaa catggctctg gtgaagtaca aaagtatccc actgaatccc
      361 attgcacagg ccgcttccat cattagagtg gtcatgggaa tcaccgctac aatttctccc
      421 gcagagtata tgaagtggct gttctttttc tttggggcca cctgcctggt gttcgagtac
      481 agtgtggtct tcacaatctt tcaagtgggg ctgtatggct ttgaatctgt cgggacacct
      541 ctggcacaga aggtggtggt gaggatcaag atgctgcgcc tgatcttctt tattgcttgg
      601 actatgttcc caatcgtgtg gctgatttca cccaccggag tctgcgtgat ccacgagaac
      661 gtgagcgcca ttctgtacct gctggctgac ggcctgtgca aaaatacata tggagtgatc
      721 ctgtggagca ctgcctgggg agtgctggaa gggaagtggg accccgcatg tctgccaggg
      781 caggagaaac ccgaagccga cgatcctttt ggcctgaatc acgagaagaa tgcccctcct
      841 aacgacgaag tcaatatcag aatgtttgga aggggacgta cggcggccaa gagcaggatc
      901 accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtggc ggtacccgtg
      961 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1021 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1081 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1141 accaccctga gctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1201 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1261 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1321 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1381 gagtacaacg ccatccacgg caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1441 aaggccaact tcggcctcaa ctgcaacatc gaggacggca gcgtgcagct cgccgaccac
     1501 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1561 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1621 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtatac cggtgcagcc
     1681 ctgcaggaga agaagtcatg cagccagcgc atggccgaat tccggcaata ctgttggaac
     1741 ccggacactg ggcagatgct gggccgcacc ccagcccggt gggtgtggat cagcctgtac
     1801 tatgcagctt tctacgtggt catgactggg ctctttgcct tgtgcatcta tgtgctgatg
     1861 cagaccattg atccctacac ccccgactac caggaccagt taaagtcacc gggggtaacc
     1921 ttgagaccgg atgtgtatgg ggaaagaggg ctgcagattt cctacaacat ctctgaaaac
     1981 agctctagac aggcccagat caccggacgt ccggagggag ctgtacaaga gctgatcagc
     2041 agcgccacca gatctctgtt tgccgccgga ggcatcaacc cttggcctaa cccctaccac
     2101 cacgaggaca tgggctgtgg aggaatgaca cctacaggcg agtgcttcag caccgagtgg
     2161 tggtgtgacc cttcttacgg actgagcgac gccggatacg gatattgctt cgtggaggcc
     2221 acaggcggct acctggtcgt gggagtggag aagaagcagg cttggctgca cagcagaggc
     2281 acaccaggag aaaagatcgg cgcccaggtc tgccagtgga ttgctttcag catcgccatc
     2341 gccctgctga cattctacgg cttcagcgcc tggaaggcca cttgcggttg ggaggaggtc
     2401 tacgtctgtt gcgtcgaggt gctgttcgtg accctggaga tcttcaagga gttcagcagc
     2461 cccgccacag tgtacctgtc taccggcaac cacgcctatt gcctgcgcta cttcgagtgg
     2521 ctgctgtctt gccccgtgat cctgatcaag ctgagcaacc tgagcggcct gaagaacgac
     2581 tacagcaagc ggaccatggg cctgatcgtg tcttgcgtgg gaatgatcgt gttcggcatg
     2641 gccgcaggac tggctaccga ttggctcaag tggctgctgt atatcgtgtc ttgcatctac
     2701 ggcggctaca tgtacttcca ggccgccaag tgctacgtgg aagccaacca cagcgtgcct
     2761 aaaggccatt gccgcatggt cgtgaagctg atggcctacg cttacttcgc ctcttggggc
     2821 agctacccaa tcctctgggc agtgggacca gaaggactgc tgaagctgag cccttacgcc
     2881 aacagcatcg gccacagcat ctgcgacatc atcgccaagg agttttggac cttcctggcc
     2941 caccacctga ggatcaagat ccacgagcac atcctgatcc acggcgacat ccggaagacc
     3001 accaagatgg agatcggagg cgaggaggtg gaagtggaag agttcgtgga ggaggaggac
     3061 gaggacacag tgctcgagca gagccagccc atcctgaaca ccaaggagat ggccccccag
     3121 agcaagcccc ccgaggagct ggagatgagc agcatgccca gccccgtggc ccccctgccc
     3181 gcccgcaccg agggcgtgat cgacatgcgc agcatgagca gcatcgacag cttcatcagc
     3241 tgcgccaccg acttccccga ggccacccgc ttctgatcta gaaagctatt cctcgagggc
     3301 ggccgctcta gagtcgacgg gccgcggtaa caattgttaa ctaacttaag ctagcaacgg
     3361 tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc gaagccgctt
     3421 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     3481 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     3541 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3601 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     3661 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     3721 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     3781 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     3841 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     3901 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc atgaccgagt
     3961 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     4021 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     4081 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4141 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4201 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     4261 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     4321 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     4381 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     4441 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     4501 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
//
