LOCUS       sequence-373155-        1875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-vector for optogenetic silencing of noradrenergic neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Wiegert Lab
  TITLE     Wiegert Lab Plasmids 2022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PRS_8-somGtACR2-eGFP
FEATURES             Location/Qualifiers
     source          1..1875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(958..960,961..1671)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(958..960,961..1671)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1000..1021)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1261..1280)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1608..1629
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggcatcac aggtcgtcta tggggaatgg gcatcaactc acaccgagtg ctacaatatg
       61 tccagaatcg actccacctt cgtctcactg ctgcagctgg tgtgggccgt ggtctcaggc
      121 tgccagacaa tcttcatgat tagcagggct cccaaggtcc cttgggagtc cgtgtacctg
      181 ccctttgtcg aatctatcac ttatgcactg gccagtactg gcaacggaac cctgcagatg
      241 agggacggca gattctttcc ttggagccga atggcctcct ggctgtgcac ctgtccaatc
      301 atgctgggac agatttctaa catggctctg gtgaagtaca aaagtatccc actgaatccc
      361 attgcacagg ccgcttccat cattagagtg gtcatgggaa tcaccgctac aatttctccc
      421 gcagagtata tgaagtggct gttctttttc tttggggcca cctgcctggt gttcgagtac
      481 agtgtggtct tcacaatctt tcaagtgggg ctgtatggct ttgaatctgt cgggacacct
      541 ctggcacaga aggtggtggt gaggatcaag atgctgcgcc tgatcttctt tattgcttgg
      601 actatgttcc caatcgtgtg gctgatttca cccaccggag tctgcgtgat ccacgagaac
      661 gtgagcgcca ttctgtacct gctggctgac ggcctgtgca aaaatacata tggagtgatc
      721 ctgtggagca ctgcctgggg agtgctggaa gggaagtggg accccgcatg tctgccaggg
      781 caggagaaac ccgaagccga cgatcctttt ggcctgaatc acgagaagaa tgcccctcct
      841 aacgacgaag tcaatatcag aatgtttgga aggggacgta cggcggccaa gagcaggatc
      901 accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtggc ggtacccgtg
      961 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1021 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1081 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1141 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1201 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1261 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1321 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1381 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1441 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1501 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1561 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1621 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaccggtcag
     1681 agccagccca tcctgaacac caaggagatg gccccccaga gcaagccccc cgaggagctg
     1741 gagatgagca gcatgcccag ccccgtggcc cccctgcccg cccgcaccga gggcgtgatc
     1801 gacatgcgca gcatgagcag catcgacagc ttcatcagct gcgccaccga cttccccgag
     1861 gccacccgct tctga
//
