LOCUS       sequence-37317-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, 
            Cuervo AM, Verkhusha VV
  TITLE     Monomeric fluorescent timers that change color from blue to red 
            report on cellular trafficking.
  JOURNAL   Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.
  PUBMED    19136976
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-02"; volume: "5(2)"; pages: "118-26. Epub 2009 
            Jan 11"
COMMENT     Sequence Label: pBAD/HisB-Medium-FT
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(128..136,137..151)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             170..880
                     /codon_start=1
                     /product="blue-to-red fluorescent timer derivative of 
                     mCherry (Subach et al., 2009)"
                     /label=Medium-FT
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHLEGSVDGHEFEIEGEGEGRPYEG
                     TQSAKLKVTKGGPLPFAWDILSPQFMYGSRAYVKHPADIPDYWKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERIYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVLLPGAYNVNIKMDITSHNEDYTIVEQCERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(315..333)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     583..602
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      121 gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagatcta tggtaagcaa
      181 gggcgaggag gataacatgg ccatcatcaa ggaattcatg cgtttcaagg tgcacctgga
      241 gggctccgtg gacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      301 gggcacccag agcgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
      361 catcctgtcc cctcagttca tgtacggctc cagggcctac gtgaagcacc ccgccgacat
      421 ccccgactac tggaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      481 cgaggatggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      541 ctacaaggtg aagctgcgcg gcaccaactt cccttccgac ggccccgtaa tgcagaagaa
      601 gaccatgggc tgggaggcct cctccgagcg gatatacccc gaggacggcg ccctgaaggg
      661 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
      721 cacctacaag gccaagaagc ccgtgctgct gcccggcgcc tacaacgtca acatcaagat
      781 ggacatcacc tcccacaacg aggactacac catcgttgaa cagtgcgaac gcgccgaggg
      841 ccaccattcc accggcggca tggacgagct gtacaagtaa gaattcgaag ct
//
