LOCUS       sequence-373171-        9413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE 
            MBP tag, and c-terminal bi-partite NLS and 6x-His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Christie KA, Guo JA, Silverstein RA, Doll RM, Mabuchi M, Stutzman 
            HE, Lin J, Ma L, Walton RT, Pinello L, Robb GB, Kleinstiver BP
  TITLE     Precise DNA cleavage using CRISPR-SpRYgests.
  JOURNAL   Nat Biotechnol. 2022 Oct 6. pii: 10.1038/s41587-022-01492-y. doi: 
            10.1038/s41587-022-01492-y.
  PUBMED    36203014
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Oct 6. pii: 10.1038/s41587-022-01492-y. doi: 
            10.1038/s41587-022-01492-y."
COMMENT     Sequence Label: pET28-SpRY-HF1-NLS-6xHis (gbr2102)
FEATURES             Location/Qualifiers
     source          1..9413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     559..578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(598..675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     881..900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     984..1003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        984..1002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1003..1027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1042..1064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5241..5261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5262..5279
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5331..5349)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5345..5392
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5429..5884
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5516..5535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5726..5747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5976..6791)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5976..6791)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6699..6718
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6783..6802
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6913..7501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7402..7421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7655..7672
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7687..7826
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7752..7771)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7912..7934
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7928..8119)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(8928..9413,1..597))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
       61 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
      121 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
      181 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
      241 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
      301 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
      361 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
      421 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
      481 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
      541 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
      601 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
      661 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
      721 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
      781 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
      841 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
      901 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
      961 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
     1021 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgaaaatcg
     1081 aagaagacaa gaagtactcg atcggattgg acattggaac caattcagtc ggctgggccg
     1141 ttattaccga cgagtataaa gttccttcca agaagtttaa ggtgcttgga aatacagacc
     1201 gccacagtat taagaagaat ttgatcggtg cgcttctttt cgacagcggc gagaccgctg
     1261 agcgtactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag aatcgtattt
     1321 gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt ttctttcatc
     1381 gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat cctatttttg
     1441 gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat catctgcgaa
     1501 aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg gccttagcgc
     1561 atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct gataatagtg
     1621 atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt gaagaaaacc
     1681 ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg agtaaatcaa
     1741 gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc ttatttggga
     1801 atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt gatttggcag
     1861 aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat aatttattgg
     1921 cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta tcagatgcta
     1981 ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc ctatcagctt
     2041 caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa gctttagttc
     2101 gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa aacggatatg
     2161 caggttatat tgatggggga gctagccaag aagaatttta taaatttatc aaaccaattt
     2221 tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa gatttgctgc
     2281 gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg ggtgagctgc
     2341 atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat cgtgagaaga
     2401 ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg cgtggcaata
     2461 gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg aattttgaag
     2521 aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca gcgtttgata
     2581 aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag tattttacgg
     2641 tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc
     2701 tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat cgaaaagtaa
     2761 ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat agtgttgaaa
     2821 tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat ttgctaaaaa
     2881 ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta gaggatattg
     2941 ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt aaaacatatg
     3001 ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat actggttggg
     3061 gagcgttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc aaaacaatat
     3121 tagatttttt gaaatcagat ggttttgcca atcgcaattt tatggcgctg atccatgatg
     3181 atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa ggcgatagtt
     3241 tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt attttacaga
     3301 ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca gaaaatatcg
     3361 ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc
     3421 gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt aaagagcatc
     3481 ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc caaaatggaa
     3541 gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat gatgtcgatc
     3601 acattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc ttaacgcgtt
     3661 ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc aaaaagatga
     3721 aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag tttgataatt
     3781 taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt atcaaacgcc
     3841 aattggttga aactcgcgcg atcactaagc atgtggcaca aattttggat agtcgcatga
     3901 atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt accttaaaat
     3961 ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt gagattaaca
     4021 attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct ttgattaaga
     4081 aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat gatgttcgta
     4141 aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat ttcttttact
     4201 ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag attcgcaaac
     4261 gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa gggcgagatt
     4321 ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag
     4381 tacagacagg cggattctcc aaggagtcaa ttcgccctaa gcgcaactca gataagctta
     4441 ttgcgcgtaa gaaagactgg gatcccaaga agtacggcgg gttcctctgg cccacggtgg
     4501 cgtacagcgt tcttgttgtt gcaaaggtag agaagggcaa gagtaagaag ctgaagtccg
     4561 taaaggagct gcttggcatc accatcatgg aacgctcttc ttttgagaag aaccctatcg
     4621 acttcttaga agctaagggc tataaggaag ttaagaaaga cctgatcatc aagcttccca
     4681 agtattcttt attcgagttg gagaatggcc gcaagcgtat gctggcgtcg gcaaagcaac
     4741 tgcagaaggg taatgagttg gccctgccga gcaagtacgt taattttctg tatttagcga
     4801 gtcactatga gaagttaaaa ggaagccccg aggacaatga gcagaaacaa ctgttcgtcg
     4861 agcaacataa gcactacttg gatgagatta ttgagcagat tagcgagttc tcaaagcgtg
     4921 taatcttagc agatgctaac ttggataagg ttctgagcgc ctacaacaag catcgcgata
     4981 agcctatccg cgaacaggcg gagaatatca ttcacctgtt cactctcaca cgtttgggcg
     5041 caccccgtgc ctttaaatat ttcgacacca cgatcgaccc taagcaatat cgcagtacca
     5101 aagaggtgct ggatgccacc cttatccatc aaagcatcac cgggttgtat gagacccgta
     5161 tcgatcttag ccagttaggc ggggacgggt cgggcggcgg cggctcgggc aagcgtacag
     5221 ccgacggaag tgagttcgag cctaaaaaga agcgtaaggt acatcaccat caccatcatt
     5281 aaatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     5341 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     5401 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     5461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5581 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5641 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5701 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5821 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5881 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5941 tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc gagcatcaaa
     6001 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     6061 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     6121 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     6181 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     6241 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     6301 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     6361 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     6421 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     6481 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     6541 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     6601 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     6661 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     6721 tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga
     6781 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     6841 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7021 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7081 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7561 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7621 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7681 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
     7741 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     7801 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     7861 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7921 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     7981 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     8041 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     8101 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     8161 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     8221 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     8281 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     8341 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     8401 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     8461 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     8521 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     8581 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     8641 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     8701 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     8761 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     8821 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     8881 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
     8941 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9001 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
     9061 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     9121 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
     9181 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
     9241 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
     9301 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
     9361 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gcc
//
