LOCUS       sequence-37318-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, 
            Cuervo AM, Verkhusha VV
  TITLE     Monomeric fluorescent timers that change color from blue to red 
            report on cellular trafficking.
  JOURNAL   Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.
  PUBMED    19136976
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-02"; volume: "5(2)"; pages: "118-26. Epub 2009 
            Jan 11"
COMMENT     Sequence Label: pBAD/HisB-Slow-FT
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..724)
                     /codon_start=1
                     /product="blue-to-red fluorescent timer derivative of 
                     mCherry (Subach et al., 2009)"
                     /label=Slow-FT
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHVEGSVNGHEFEIVGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSRAYVKHPADIPDYWKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDVEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(292..311)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     561..579
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(join(743..757,758..766))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(770..802)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(806..823)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagcttcgaa ttcttacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg
       61 cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga
      121 tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga
      181 cctcaacgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca
      241 gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct
      301 gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact
      361 cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca
      421 tcacgcgctc ccacttgaag ccctcgggga aggacagctt ccagtagtcg gggatgtcgg
      481 cggggtgctt cacgtaggcc ctggagccgt acatgaactg aggggacagg atgtcccagg
      541 cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg
      601 ggcggccctc gccctcgccc acgatctcga actcgtggcc gttcacggag ccctccacgt
      661 gcaccttgaa acgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
      721 ccatagatct cgagctcgga tccttatcgt catcgtcgta cagatcccga cccatttgct
      781 gtccaccagt catgctagcc ataccatgat gatgatgatg atgagaaccc c
//
