LOCUS       sequence-373180-       12606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP 
            cargo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL,
            Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang
            K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM,
            Banfield JF, Doudna JA
  TITLE     Species- and site-specific genome editing in complex bacterial 
            communities.
  JOURNAL   Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7.
            Epub 2021 Dec 6.
  PUBMED    34873292
REFERENCE   2  (bases 1 to 12606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-021-01014-7"; journalName: "Nat Microbiol"; date: 
            "2022-01"; volume: "7"; issue: "1"; pages: "34-47"
COMMENT     Sequence Label: pBFC0619
FEATURES             Location/Qualifiers
     source          1..12606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(89..94,95..112,113..119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             120..131
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2216..2227
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(8716..8721,8722..8738,8739..8744)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             8933..9466
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     RBS             9825..9836
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             9843..10556
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      10568..10639
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10655..10682
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     10693..10712
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10693..10711
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            10855..10963
                     /note="incP origin of transfer"
     promoter        11156..11260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11261..11329,11330..12121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11479..11498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12157..12545
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
       61 ttccactgac acctgcccga accatatctt tacactttat gcttccggct cgtatgttga
      121 aagaggagaa aggatcaatg gcgacaagtt tacctacgcc ctcagcaatt acgacttcgg
      181 cgttagagta tgcattccat actcccgctc gcaatctaac gaaatctcgc ggaaaaaaca
      241 ttcatcgtta tgtcagtgta aagatgagta agaggattac ggtagaatct actctagagt
      301 gtgatgcctg ctatcacttt gattttgagc caagtattgt tcgcttttgc gctcaaccga
      361 ttcgattttt atattatctc aatggtcagt ctcactccta tgttcctgac tttctagttc
      421 aatttgatac caacgagttt gttctatatg aagtaaagtc agcttatgct aagaacaaac
      481 ctgattttga tgttgaatgg gaggcgaaag taaaagcagc aactgaacta gggctagaat
      541 tggagcttgt tgaagagagt gatattaggg atacggttgt attaaataat cttaagcgca
      601 tgcatcgtta tgcttcgaaa gatgagctga ataacgtaca taactctctc ttaaaaataa
      661 taaagtacaa tggcgcccaa tctgcaagat gcttgggaga acagttgggt ttaaaaggcc
      721 gaactgtttt accaattttg tgcgatttgc tgtcaaggtg tttactcgat acacgtttgg
      781 ataagcctct atctcttgaa tctcgatttg agttggccag ttatggctaa gaaagggttc
      841 tcaagtttcc atagaaaagc agtctcttcc caagatacgc ttgaatcgat agagcttgtc
      901 tctagcgcta attgtctaga aagtgttacg tatcaagata tatcagcatt tcccgaaaca
      961 attgcggtag agattaattt ccgattaagc attcttcgct ttttagcgcg aaagtgcgaa
     1021 accattgtgg ccaaatcaat tgaaccacat cgtgtagagc tacagcaaaa ctatagtaga
     1081 aaaataccca gtgcaataac gatatatcga tggtggcttg cttttcgaaa atcagactac
     1141 aaccccatta gcttagcacc taatatcaag gatagaggta atagagagac aaaagtgtca
     1201 acagttgttg attctattat ggaacaggca gttgaaagag ttatatctgg acgaaaagtc
     1261 aatgttagct ctgcatataa acgtgttcga cgaaaagttc gtcaatacaa tctaactcat
     1321 ggaacgaaat acacgtatcc taagtacgaa tctgtaagaa agcgagtaaa aaagaaaacc
     1381 ccatttgagt tattagccgc agggaaaggg gagagagtag ctaagagaga gtttcgccga
     1441 atgggaaaaa agatcctcac gtctagcgtg ctagagaggg ttgaaataga tcacactgtc
     1501 gttgaccttt ttgcagtaca cgaagagtat cgaatcccat tgggccgacc ttggcttact
     1561 caattggttg attgttacag taaagctgta atcggttttt atttaggttt cgagcctcct
     1621 agctatgtgt cggtttccct tgcacttaag aatgcaatac aacgcaaaga tgacttaatc
     1681 tcctcgtatg aatcgatcga gaatgaatgg ctatgttatg gcatcccaga cctactcgta
     1741 actgataatg gtaaagagtt tttgtcgaaa gcatttgatc aagcatgtga atcactattg
     1801 atcaatgtgc atcaaaataa agttgaaacg cccgacaaca aacctcatgt tgaacgtaac
     1861 tacgggacta ttaatacttc tctgttagac gatttacctg ggaaatcctt cagccagtac
     1921 cttcaaagag aagggtacga ctctgtggga gaagctaccc ttacactcaa tgagattaga
     1981 gaaatttact taatttggtt ggtggatatt tatcataaaa aacccaatca gagaggcact
     2041 aattgtccta atgttgcgtg gaaaaagggt tgtcaagaat gggaaccaga ggagttctct
     2101 ggttctaaag acgaattaga ctttaaattt gctattgttg attacaaaca acttactaaa
     2161 gtagggataa ctgtctacaa agaactgagt tatagcaatg accgtttagc tgaatataga
     2221 gggaagaaag gaaaccataa agttcagttc aagtataacc ctgagtgtat ggcagttatt
     2281 tgggtgttgg atgaggatat gaatgagtac tttacagtta atgcgattga ctacgaatat
     2341 gcaagtagag tatcactttg gcaacataaa tataacatga aatatcaagc agaactaaat
     2401 tcagcagaat atgatgagga caaggaaatt gatgcagaaa taaaaattga agaaatcgca
     2461 gatcgttcaa ttgttaagac taacaaaatc agagctcgga ggcgtggcgc taggcatcaa
     2521 gagaatagcg caagggctaa gtcaatcagt aatgcgaacc cggcctcgat acaaaaacat
     2581 gaagatgaaa tcgttagtgc agataatgac gattgggata ttgattatgt ctgagaaatc
     2641 gtcaaatgag tgaaacgcgt gaggctcgta tatcaagagc taaaagggca tttgtatcca
     2701 caccgagcgt taggaaaatc ttgagttaca tggatagatg tagagatcta tcagacctag
     2761 agtctgagcc tacatgcatg atggtctatg gtgcttcggg tgtaggtaaa acgaccgtca
     2821 tcaagaaata cttaaatcaa aacagaagag agtccgaagc cgggggcgat ataataccgg
     2881 ttttgcatat tgagttgcca gacaatgcga agccagtaga tgcagcaagg gaattgctgg
     2941 ttgaaatggg tgacccgcta gcactttatg aaactgactt agctagattg acgaaaagac
     3001 tgactgaatt aatccctgcg gtcggcgtga agctgattat tatcgatgag ttccaacatt
     3061 tggtggaaga aaggtcaaat cgggttctta cccaagtagg taattggcta aaaatgatac
     3121 ttaacaaaac gaaatgtcca attgttatat ttggtatgcc atactcaaaa gttgtactgc
     3181 aagcaaactc gcaacttcac gggcgatttt ccattcaggt tgaactgcgc ccctttagct
     3241 accagggagg tagaggtgta tttaaaactt ttttggaata ccttgataaa gccctacctt
     3301 ttgaaaaaca ggctggctta gccaacgaaa gtttgcagaa aaaattgtat gcattctctc
     3361 agggaaacat gcgttcgttg agaaacctta tttatcaagc atctatcgaa gcaattgata
     3421 atcagcatga aacgataacc gaagaagatt tcgtttttgc atcgaagttg acatcgggcg
     3481 ataaacccaa ctcatggaaa aatccttttg aggagggtgt tgaggtaaca gaagatatgt
     3541 tacgaccgcc accaaaagat attggttggg aggactattt gagacattca accccgagag
     3601 tgagtaaacc aggtagaaat aaaaactttt tcgaataacc taggctgctg ccaccgctga
     3661 gcaataacca tcttagtata ttagttaagt ataagaagga gatatacata tgtttttgca
     3721 aagacctaaa ccttacagcg atgaaagttt agaaagtttc tttatccgag tggctaacaa
     3781 aaatggctac ggtgatgtcc atcgcttcct agaagccact aaacgattcc ttcaagacat
     3841 tgaccataat ggctatcaaa cctttccgac tgatataact cggataaacc catactcagc
     3901 taaaaacagt tccagcgcac gaactgcgtc attcctgaaa cttgcacaat tgacatttaa
     3961 tgaaccgcca gagctacttg ggttggcaat taacagaaca aacatgaaat actcgccgtc
     4021 aactagcgcg gttgttagag gtgcagaagt ctttcctcgc agtttactac ggacgcactc
     4081 catcccctgc tgtcctttgt gtctgcgaga aaatggctac gcctcctacc tttggcactt
     4141 tcaggggtac gaatactgcc acagccataa cgtaccttta attaccactt gtagctgtgg
     4201 taaggagttt gactaccgag tatctgggtt aaagggcatt tgctgcaaat gcaaggagcc
     4261 tatcacctta accagcaggg agaacggtca tgaggcagcg tgtactgttt caaactggct
     4321 tgctggccat gaatctaaac ctctgccaaa tcttcctaaa agctaccgat ggggtttagt
     4381 tcattggtgg atgggtatta aagatagcga gttcgatcac ttttcgttcg ttcaattttt
     4441 ctcaaactgg ccaaggtcat tccactcgat aatcgaagat gaagtagagt tcaaccttga
     4501 gcatgctgtt gtcagcacgt ctgaattacg actaaaagat cttcttggtc gattgttttt
     4561 cggttcaatt cggttacctg agcggaatct tcaacacaat atcatccttg gtgagcttct
     4621 ctgctattta gaaaatcgtt tatggcaaga caagggatta atcgccaacc tcaaaatgaa
     4681 cgcgttagag gcgactgtaa tgttaaattg tagcctcgat cagattgcat caatggttga
     4741 acaacgcatc ttgaagccaa atcgaaaaag caagcccaac agccctcttg atgttaccga
     4801 ttatctattt catttcggcg atattttctg tctttggtta gctgagttcc aaagcgatga
     4861 gtttaaccgt tcgttttatg tgtcgaggtg gtaaatgcaa actctgaaag aactaatcgc
     4921 atccaatcct gacgacttaa caactgaact taagagagca tttcgtccac tcacaccgca
     4981 tattgcaatt gatggtaatg aacttgacgc actgacgata ttagtcaatt taaccgataa
     5041 gactgatgat cagaaagacc tgctcgatcg agccaaatgc aagcaaaaac ttcgagatga
     5101 aaaatggtgg gctagctgca taaattgcgt taattacaga caaagccata acccaaaatt
     5161 cccggatata cgttctgaag gcgtgattcg aacccaagcc ctgggtgaat taccgagttt
     5221 cctactctcg tcctcaaaaa tcccaccata ccattggtca tatagccatg attcaaaata
     5281 cgtcaacaaa agcgcattcc tcaccaatga gttttgttgg gatggtgaga tctcatgttt
     5341 gggtgagctt cttaaagatg cagatcaccc actttggaat actttgaaaa agttaggttg
     5401 ttctcaaaaa acttgcaaag caatggcaaa acaactagct gatattactc tcacgactat
     5461 caatgtcaca ctcgcaccaa attacctgac tcaaatatct ctccccgata gtgatacatc
     5521 ctacatttca ctctctcctg ttgcatcgct atcgatgcaa agccactttc atcagaggct
     5581 ccaagatgaa aatcggcata gcgcgataac gcggtttagc cgaactacca acatgggggt
     5641 cactgcgatg acatgtggcg gcgcatttag aatgttaaag tctggcgcta agttttccag
     5701 cccccctcat caccgattaa acagtaaacg aagttggttg acgtcagagc atgttcagtc
     5761 attaaaacag taccagcgcc tcaacaaaag cctcatacct gaaaactctc ggattgcact
     5821 ccgtagaaaa tacaaaatcg agcttcaaaa tatggtcaga tcttggtttg caatgcaaga
     5881 ccataccctt gattcgaaca tacttatcca acatttgaat catgacctat cttacttagg
     5941 agccacaaaa cgttttgcat acgatccagc gatgaccaag ctctttactg agcttttgaa
     6001 acgagagtta tcaaattcaa tcaataatgg tgagcaacac actaatggat cgtttttagt
     6061 cctaccgaat atcagggttt gtggcgcaac agctttaagc tccccggtaa cggtggggat
     6121 tccatcactt acagctttct ttggcttcgt tcacgcattt gaacggaata taaatcgcac
     6181 cacctcatcg tttcgtgttg aatcctttgc gatatgcgtc catcaactac atgtcgagaa
     6241 gcgaggtttg acagcagagt ttgtggaaaa aggcgacggg actatatccg ctcccgcgac
     6301 ccgggatgac tggcagtgtg atgtcgtatt tagccttatt ttgaacacca actttgctca
     6361 acatattgac caagatacgt tagttacatc actaccaaag cgattggctc ggggttcagc
     6421 aaaaattgcg attgatgact ttaaacatat caactcattc tcgacattag aaacagcgat
     6481 cgaatctctg ccaatagaag ctggtaggtg gttatcactt tacgcacagt caaacaataa
     6541 tctaagtgat ctattagcag ccatgacaga ggaccatcag ctcatggcaa gctgcgtcgg
     6601 ttaccacttg ttagaagaac ccaaagataa accaaactcc ctcagaggtt acaaacacgc
     6661 tatcgccgag tgcatcattg gactcattaa ctcaatcacc tttagctcag agactgatcc
     6721 caacacaatc ttttggtcgc taaagaacta tcaaaactac ctagtggtac agccaaggag
     6781 tatcaacgat gaaactaccg acaaatctag cctatgagcg ctctatcgac ccatcagatg
     6841 tctgtttttt tgtcgtctgg cccgatgata gaaaaacacc tttaacctac aattctcgta
     6901 ctctgctcgg gcaaatggaa gcggcatcat tagcctatga tgtctcaggt caaccaataa
     6961 aaagtgccac cgctgaggcg ttagctcaag ggaaccctca tcaagttgat ttctgccacg
     7021 ttccatacgg tgcgagtcat attgaatgca gtttctccgt ttcgttttct tctgaactac
     7081 gtcaaccata taagtgtaac tcaagcaaag ttaaacaaac gctagtgcaa ttagtcgagc
     7141 tctacgaaac gaaaatcggc tggactgagc tagcaacccg atatttgatg aacatttgca
     7201 acggtaaatg gctgtggaaa aatacccgta aagcctattg ctggaacatt gtacttacac
     7261 cttggccttg gaacggggaa aaggttggat ttgaagatat tcgtactaac tacacctcac
     7321 ggcaagactt taaaaataat aaaaattggt ctgctatagt tgaaatgatc aaaaccgcat
     7381 tttctagtac tgatgggctg gcgatatttg aagtcagggc caccttgcac ttgccaacga
     7441 atgctatggt gcggccaagc caagttttca cagaaaaaga aagtggcagt aaaagtaaat
     7501 ctaaaactca aaacagtcga gtttttcaga gtacaactat tgatggtgaa cgatcgccaa
     7561 tactaggggc ctttaaaacg ggagcagcta ttgcaaccat tgacgactgg tatcctgaag
     7621 ccactgagcc actaagggtc ggacggtttg gggttcatcg cgaagatgtc acttgctacc
     7681 gtcatccgtc taccggaaaa gattttttct cgatattaca acaagcagag cactatattg
     7741 aagtgttgag cgccaacaaa actcccgctc aagaaactat caacgacatg cactttttaa
     7801 tggctaacct gattaagggt gggatgttcc agcataaagg agactgactg tgaaatggta
     7861 ttataagaca atcacctttc tgccagagtt gtgcaacaac gagtcactgg ctgcaaagtg
     7921 tctccgcgtt ctgcatggat ttaactatca gtatgagaca cgaaatatcg gcgtttcatt
     7981 tccgctttgg tgtgatgcaa cggttggaaa aaagatttca tttgtcagca agaacaagat
     8041 agaactcgac ttactactta aacaacacta tttcgtccaa atggaacaac ttcaatactt
     8101 tcatatatcc aacactgttc tcgtcccaga agattgtaca tacgtttcct ttagacgctg
     8161 tcaatctata gataagctca cagcagcagg gctggcaagg aaaatcagac gcctggagaa
     8221 acgtgctcta tctagaggcg agcaatttga cccatcatct tttgctcaaa aagagcatac
     8281 tgcaatagcg cactaccact cacttgggga gtccagcaaa cagacgaacc gcaactttcg
     8341 actcaatatc aggatgctct cggagcaacc ccgtgagggg aactcgattt ttagtagcta
     8401 tggcttatca aattcagaaa actcgtttca gcctgtaccc ttaatctgac ctaggctgct
     8461 gccaccggtg aactgccgag taggtagctg ataacgagac ctcgtttacc tatcggtctc
     8521 gtgaactgcc gagtaggtag ctgataaccc aaaccagatg tcaacacagc tactgttgat
     8581 gcaaccataa agtgatattt aataattatt tataatcagc aacttaacca caaaacaacc
     8641 atatattgat atctcacaaa acaaccataa gttgatattt ttcagccagg ggtccccaat
     8701 aattacgatt taaatttgac ataagcctgt tcggttcgta aactgtaatg caagtagcgt
     8761 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     8821 gcggttttca tggcttgtta tgactgtttt tttgtacagc ctatgcctcg ggcatccaag
     8881 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     8941 cagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag gtggctcaag
     9001 tatgggcatc attcgcacgt gtaggctcgg ccctgaccaa gtcaaatcca tgcgggctgc
     9061 tcttgatctt ttcggtcgtg agttcggtga cgtagccacc tactcccaac atcagccgga
     9121 ttccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg ctgccttcga
     9181 ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg agcagccgcg
     9241 tagtgaaatc tatatctatg atctcgcagt ctccggagag caccggaggc agggcattgc
     9301 caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt atgtgatcta
     9361 cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt tgggcatacg
     9421 ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaacaat tcgttcaagc
     9481 cgagatcggc ttcccggccg cggagttgtt cggtaaattg gacaacggtc cgcgcgttgt
     9541 ccttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat
     9601 cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta
     9661 tccaacggcg tcagccgaaa gtgaaacgtg atttcatgcg tcattttgaa cattttgtaa
     9721 atcttattta ataatgtgtg cggcaattca catttaattt atgaatgttt tcttaacatc
     9781 gcggcaactc aagaaacggc aggttcggat cttagctact agagaaagag gagaaatact
     9841 agatgcgtaa aggcgaggag ctgttcactg gtgtcgtccc tattctggtg gaactggatg
     9901 gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg
     9961 gtaaactgac gctgaagttc atctgtacta ctggtaaact gccggttcct tggccgactc
    10021 tggtaacgac gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc
    10081 agcatgactt cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct
    10141 ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg
    10201 taaaccgcat tgagctgaaa ggcattgact ttaaagagga cggcaatatc ctgggccata
    10261 agctggaata caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg
    10321 gcattaaagc gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg
    10381 atcactacca gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact
    10441 atctgagcac gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc
    10501 tgctggagtt cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaatgac
    10561 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
    10621 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
    10681 cgtttatagc tgtaatacga ctcactatag gggataagag acggctcaac gcccgtctca
    10741 cagcgcaata tcaatttatg ggtgtgataa ttatcaattt atgggtgtaa ttatcatttt
    10801 atggttgtat caacaaggag gactttggca ggtgcctgca ggattcttgc ttatcggcca
    10861 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
    10921 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
    10981 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
    11041 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagaa
    11101 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
    11161 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    11221 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    11281 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    11341 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    11401 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    11461 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    11521 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    11581 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    11641 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    11701 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    11761 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    11821 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    11881 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    11941 gtttattgct gataaatctg gagccggtga gcgtggctct cgcggtatca ttgcagcact
    12001 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    12061 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    12121 actgtcagac ccggccacga tgcgtccggc gtagaggatc tgaagatcag cagttcaacc
    12181 tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac
    12241 taagctctca tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta
    12301 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata
    12361 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata
    12421 taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc
    12481 actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc
    12541 tgacatgggc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    12601 aaaagg
//
