LOCUS       sequence-37320-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz 
            J, Verkhusha VV
  TITLE     Photoactivatable mCherry for high-resolution two-color fluorescence 
            microscopy.
  JOURNAL   Nat Methods. 2009 Feb;6(2):153-9. Epub 2009 Jan 25.
  PUBMED    19169259
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2009-02"; volume: "6(2)"; pages: "153-9. Epub 2009
            Jan 25"
COMMENT     Sequence Label: pBAD/HisB-PAmCherry1
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             71..88
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(128..136,137..151)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             170..880
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein"
                     /label=PAmCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(315..333)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     583..602
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg ttttttgggc taacaggagg aattaaccat
       61 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      121 gggtcgggat ctgtacgacg atgacgataa ggatccgagc tcgagatcta tggtgagcaa
      181 gggcgaggag gataacatgg ccatcattaa ggagttcatg cgcttcaagg tgcacatgga
      241 ggggtccgtg aacggccacg tgttcgagat cgagggcgag ggcgagggcc gcccctacga
      301 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcacctggga
      361 catcctgtcc cctcaattca tgtacggctc caatgcctac gtgaagcacc ccgccgacat
      421 ccccgactac tttaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaaatt
      481 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gtgagttcat
      541 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      601 gaccatgggc tgggaggccc tctccgagcg gatgtacccc gaggacggcg ccctgaaggg
      661 cgaggtcaag ccgagagtga agctgaagga cggcggccac tacgacgctg aggtcaagac
      721 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca accgcaagtt
      781 ggacatcacc tcacacaacg aggactacac catcgtggaa cagtacgaac gtgccgaggg
      841 ccgccactcc accggcggca tggacgagct gtacaagtaa gaattcgaag cttggctg
//
