LOCUS       sequence-373206-       12559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An. gambiae transgenesis plasmid. PiggyBac with transposase in 
            backbone. Expresses two gRNAs targeting fle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12559)
  AUTHORS   Smidler AL, Pai JJ, Apte RA, Sanchez C HM, Corder RM, Jeffrey 
            Gutierrez E, Thakre N, Antoshechkin I, Marshall JM, Akbari OS
  TITLE     A confinable female-lethal population suppression system in the 
            malaria vector, Anopheles gambiae.
  JOURNAL   Sci Adv. 2023 Jul 7;9(27):eade8903. doi: 10.1126/sciadv.ade8903. 
            Epub 2023 Jul 5.
  PUBMED    37406109
REFERENCE   2  (bases 1 to 12559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-7"; volume: "9(27):eade8903. doi"; pages: " 
            10.1126/sciadv.ade8903. Epub 2023 Jul 5"
COMMENT     Sequence Label: 1154B_gFLE_pB_VTK_ActG
FEATURES             Location/Qualifiers
     source          1..12559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    993..1026
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_RNA        1404..1479
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(4682..4684,4685..4687,4688..5398)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4727..4748)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4988..5007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5335..5356
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5528..5609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5565..5584)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    5635..5668
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5672..5688
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5673..5689
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5972..6006
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6497..6513)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6497..6513)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6510..6532)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6521..6537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6545..6551,6552..6569,6570..6575))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6590..6611
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6728..6745)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10881..11469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10961..10980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(11687..11706)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             11698..12507
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(11765..11784)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gtgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa acaaaaactc aaaatttctt
      961 ctataaagta acaaaacttt tatcgaattc atataacttc gtataatgta tgctatacga
     1021 agttatggat ccactagatc ccaaagagac gcttattatg gccacatata cactgaagcg
     1081 gtttggctct tggttattct agaaaactct gtttttgaat taatcatagg aaaactgttt
     1141 attctttttt gatatttcaa cgtattacaa tgaatagtaa cttttgatta aaaaagagat
     1201 gaagcaatac aagaaatgtt gaaaagttta tgaaacatca cattagcgtg agttacggca
     1261 ggatcaaaac cttataacag tcacactcag gcaaaaaatc cttcttgaat atcctttatg
     1321 catcgctaga gcaaggattg aaagcgcaaa gtatatatac aacctttttt cccctcgtcc
     1381 ttgcgacggc tcgttcatcg ctggttttag agctagaaat agcaagttaa aataaggcta
     1441 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgctg acggcggaag
     1501 cgtcatacac cgtgggggat agtcaccgaa aaaaatgcac cgactcggtg ccactttttc
     1561 aagttgagta cggactaagc cttattccaa cttgctatgt ttccatagct ctggaactac
     1621 caggcgacgc gctcgatcaa ggacgagggg aaaaaaggtt gtatatatac tttgcgcttt
     1681 caatccttgc tctagcgatg cataaaggat attcaagaag gattttttgc ctgagtgtga
     1741 ctgttataag gttttgatcc tgccgtaact cacgctaatg tgatgtttca taaacttttc
     1801 aacatttctt gtattgcttc atctcttttt taatcaaaag ttactattca ttgtaatacg
     1861 ttgaaatatc aaaaaagaat aaacagtttt cctatgatta attcaaaaac agagttttct
     1921 agaataacca agagccaaac cgcttcagtg tatatgtggc gacgaatcta cgtcacgacg
     1981 cttctagttc tagtgttccc acaatggcgc atgtgcttgt gtgtgaggcg tctctctctt
     2041 cgtctctgtt gcgcaaacgc atagactgca ctgaaaaaat cgattaccta ttttttatga
     2101 atgaatattt gcactattac tattcaaacc tattaagata gcaatcacat tcaatagcca
     2161 aatactatac cacctgagcg atgcaacgaa atgatcaatt tgagcaaaaa tgctgcatat
     2221 ttaggacggc atcattatag aaatgcttct tgctgtgtac ttttctctcg tctggcagct
     2281 gtttcgccgt tattgttaaa accggcttaa gttaggtgtg ttttctacga ctagtgaatg
     2341 ccctactaga agatgtgtgt tgcacaaaat gtccctggaa taaccaattt gaagtgcaga
     2401 tagcagtaaa cgtaagctaa tatgaatatt atttaactgt aatgttttaa tatcgctgga
     2461 cattactaat aaacccacta taaacacatg tacatatgta tgttttggca tacaatgagt
     2521 agttggggaa aaaatgtgta aaagcaccgt gaccatcaca gcataaagat aaccagctga
     2581 agtatcgaat atgagtaacc cccaaattga atcacatgcc gcaactgata ggacccatgg
     2641 aagtacactc ttcatggcga tatacaagac acacacaagc acgaacaccc agttgcggag
     2701 gaaattctcc gtaaatgaaa acccaatcgg cgaacaattc atacccatat atggtaaaag
     2761 ttttgaacgc gacttgagag cggagagcat tgcggctgat aaggttttag cgctaagcgg
     2821 gctttataaa acgggctgcg ggaccagttt tcatatcact accgtttgag ttcttgtgct
     2881 gtgtggatac tcctcccgac acaaagccgc tccatcagcc agcagtcgtc taatccagag
     2941 acaccaaacc gaaagactta atttatattt atttaattaa ttttaataaa acacaccaaa
     3001 tgtaagtagc tttccccttc ccaacaacaa aacaccatcg aaccactccc accaagaaaa
     3061 agcaataatc gagaaaagcc gcggaaaatg tgtgattttt tttgtaaaca aaacattttt
     3121 ttatgtgcca gtgctgaaag tgatcaaaaa atactagcca cgagctaaag agttattgta
     3181 ttgaccaaaa ctccaaaaat acccaagttt ggccctaaat tgtcaatcaa aataccaata
     3241 ggtcgaaaga catcaaaatt aacaaaacca gggtttcaaa taccataact caagaatcag
     3301 gattacaact gcagatttca ggatatatac atacaaatta tacgaaatta taaaaaccaa
     3361 agcaattcaa tagccccaac tcaaatgtta ggatctaata tagtgtttaa agccaagctc
     3421 gctgatgtgg gcgtgtcacg atttcaccca aagatatgcc aaattacgaa ttgcaaatca
     3481 attcgccaac actttctttt tttcccacgc cctaaaacac cagatcatcc ataaatgtac
     3541 atacatacag tatatgcata ttataatcta taaaactaga tcaggttctt gaaaatagtg
     3601 acgtaggcag ccgttttggc ttaagcagaa atttttgccg gtttttcaaa gttgtagttg
     3661 caaaaatgga gaaaaccttc gagcattcgt tcatgtacac acactcacgc gcaaaataac
     3721 gagagagagt gtatgtgtgt gtgagagagc gaaagccaga cgacggtttg cttttcgcct
     3781 cgaaacatga ccatatatgg tcacaaaact tggccgccgc aattcaacac accagcgctc
     3841 tccttcgcac ccatagcgac catggcgcgg agcgagcgag atggcgagag cgagcgacgc
     3901 ctatggcgac gtcgacgcag gcagcgattg aaaaacgcag ttaactggca ttcaacattc
     3961 accagccact ttcagtcggt ttattccagt cattcctttc aaaccgtgcg gtcgcttagc
     4021 tcagcctcgc cacttgcgtt tacagtagtt ttcacgcctt gaatttgtta aatcgaacaa
     4081 aaaggtaaag tttaactagc tttgaaaagt ttcgtggctc ttaattgtta aattttctag
     4141 agtgcgttta gtgttttttt ttttattttg taatgttaat ttcgggttcc aattcgagtt
     4201 ttaggcagcc gccattttaa gggcgcatac acacaggcaa ctgtgctctc tttgcggctt
     4261 tcttttgcac cggcattcgt taagtgtcgt ctagaagctt ctcccctccc ttttcggcat
     4321 attcgtattg tggttttaat ttttcggggc ggggccttct attttgtaac tgttctttta
     4381 atttcttatt acaattcgat cgcaagtgaa aatcagtttt caatcggaaa agtatttttt
     4441 tatgaaattt ttttttgtcc aagattaaaa ttttgtacta aaaaaacgta cattgcattg
     4501 agtgattttt aattgtacac gaaaaacaag ttagtttgtt atgacaattg tattttggta
     4561 aatcagcgca gtccaaggaa accacgcaaa ttctcagttt ttttttttgc catttctaca
     4621 ttaccaaata aggtaaccaa aaactaatgg gaaatccgca ttctttccat tgcagcttac
     4681 aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4741 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     4801 cggcaagctg accctgaagt tcatctgcac caccagcaag ctgcccgtgc cctggcccac
     4861 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     4921 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4981 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5041 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5101 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     5161 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     5221 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5281 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5341 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     5401 cggatgacgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
     5461 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     5521 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5581 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactataact
     5641 tcgtataatg tatgctatac gaagttatta ctcgaggtcg acggtatcga taagcttgat
     5701 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca
     5761 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt
     5821 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc
     5881 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta
     5941 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt
     6001 ctagggttaa tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat
     6061 cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag
     6121 gttgaagcgg catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga
     6181 aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa
     6241 ctgttcgttc aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg
     6301 atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt
     6361 gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg
     6421 tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc
     6481 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6541 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6601 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6661 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6841 tgcgatgtag aacgcgagca aattcttttc cttccatgac agcagcagcc acagtgggaa
     6901 gcctaacgtc agacgtgttt gacatgccga gctgggcggg aaaattaccg cgtgcgcatt
     6961 gttttcaagc aaatcacaac tcgctgcaaa caaaaccgtt gagaaattga ttgttttata
     7021 atttgtattt tattttattt gttataataa actaaaaaga catacttttt gcatttttta
     7081 tacataaaaa catacataca gcattataaa acacatataa gccctccctg tagagtcccg
     7141 tgtcgaaatc ttccatccaa gttgcacagt acgacggacg agtaggccgt gtgggtgcaa
     7201 aatccagcat tttgctgtct tttgctcgga gtactctcgg cgagtcggag gtttctgctg
     7261 acgtgcttag cgctaaatta gccaattgct tttgcaagtg aaataaccag ccgaatagta
     7321 cttcaaaact caggtaagtg aactagtttt atagaacaaa tgtttgtttg ttagaagtta
     7381 gtgaagtgtt tgtgaaaaaa atctctcatt tcggcaaaac taacgtaact gatttcaaat
     7441 tgaattattg ttttgtgatg ttatattatt tcatccagtt gattagtatt ttcttagtta
     7501 tgttcaaaat acagttaaat taaatttcat ttcatttact cataaaataa tctcttggct
     7561 tatttaattt ttctcgaatt tgcttgtatt gttcagtagc acgcgccatt cgccctttgt
     7621 tacattttgt acctgctccc actaacacac tggcagtgcg aaacaaaagc cttcgcacgc
     7681 gttgctggta ttagagtgtg tgcgtgtgtg tgttgagcgc tctgtcaaaa tcggctgttg
     7741 ccgccggtac cgaaattgcc tgttcgcacg ctgttcgtaa acattccgtg gtgtgtatcg
     7801 tgtgttgtgc atattgcgcg cctccccccc ttatgatagc aaaaggttgc cgtggtgtgt
     7861 ggcgcagttg agtttttgga ttaattttct aaggaaatgg cacgagaaga gcggtggcag
     7921 tgtgttggtt tgctctgtcc cttcctttct gtgtgaagtg ttcttacagc acagcacgta
     7981 tccaccaccg cacacagagc aggcaaggaa gtggaagtga acaagtgtgc tgcgcatgca
     8041 tgtgtgtggg gggcatttta gctgagatcg tcgttatttg agaagcggta taggggccag
     8101 tcggtgtcga cgtacggaag cgatttagtt ttaatccaag cgtatcccgt cgtggagtgg
     8161 ttgtgtggct ctgtgtgctc tcatatcagt tcctgagtga ggttagtaga atcacagtcc
     8221 ttggcctttt tcgttacaag atatccagaa ggatggcgtt atttccacag cttaccatgg
     8281 tgctcttgtg tgctcgaatc aggggtgaaa atccgttttg tgtttcatga accgcagttg
     8341 gcactggagc ggattcataa gtcttcgata tgcaatagat aagagagacg ttggggcata
     8401 gttgggaagc tttccgagat gtggagtttc cgagaggaga aatggtgctt tcgtgcacgt
     8461 tccgggacag cgggccccgc gaagagcatc tcgttgtcgt tcatccggca ataattgatg
     8521 cgaaaagcgc gcgcgccact ggcttagcgc agtgtacaca gtgatattca cctacacaca
     8581 cagaggcaca cgccttcaca cgcgcgcgtg cttcaaaggc tacttcggta gcggtgtgtg
     8641 aggtcgcttg caatggacaa tgaaaatttc gctggaaaat accatcgtcg ctttaggttg
     8701 ctatgagtgc gggtagagcg gtggtcgtcg atagtggtgg tgtagtgtgt gtgtgtgcaa
     8761 cggcaattat tttttataat atttcgacca tctttctttc tctctctcca cgtgctgctg
     8821 ctgctgcatt gcatgttcca ctattcctct cggtttgtgc ctgcggacgc cattgctagt
     8881 cgaaagagag tcgccgttag tcgcgcttcg agcaacggac acgttttttg gttgaaacca
     8941 acagcttttt tcatcttcgg gagacacaca gatctcgaat cgtacattcc caaaggagaa
     9001 ttgtcatctt ccggtgaata aagaaaggaa acaatatggg ctctagcctg gacgacgagc
     9061 acatcctgag cgccctgctg cagagcgacg acgaactggt gggcgaggac agcgacagcg
     9121 aggtcagcga ccacgtgtcc gaggacgacg tgcagtccga caccgaggaa gccttcatcg
     9181 acgaggtgca cgaagtgcag cctaccagca gcggctccga gatcctggac gagcagaacg
     9241 tgatcgagca gcctggcagc tccctggcca gcaacagaat cctgaccctg ccccagagaa
     9301 ccatcagagg caagaacaag cactgctggt ccacctccaa gagcaccagg cggagcagag
     9361 tgtccgccct gaacatcgtg cggagccaga ggggccccac cagaatgtgc agaaacatct
     9421 acgaccccct gctgtgcttc aagctgttct tcaccgacga gatcatcagc gagatcgtga
     9481 agtggaccaa cgccgagatc agcctgaaga ggcgggagag catgaccagc gccaccttca
     9541 gagacaccaa cgaggacgag atctacgcct tcttcggcat cctggtgatg accgccgtga
     9601 gaaaggacaa ccacatgagc accgacgacc tgttcgacag atccctgagc atggtgtacg
     9661 tgtccgtgat gagcagagac agattcgact tcctgatcag atgcctgaga atggacgaca
     9721 agagcatcag acccaccctg cgggagaacg acgtgttcac ccccgtgcgg aagatctggg
     9781 acctgttcat ccaccagtgc atccagaact acacccctgg cgcccacctg accatcgatg
     9841 agcagctgct gggcttcaga ggcagatgcc ccttcagagt gtacatcccc aacaagccca
     9901 gcaagtacgg catcaagatc ctgatgatgt gcgacagcgg caccaagtac atgatcaacg
     9961 gcatgcccta cctgggcaga ggcacccaga caaacggcgt gcccctgggc gagtactacg
    10021 tgaaagaact gagcaagcct gtgcatggca gctgcaggaa catcacctgc gacaactggt
    10081 tcaccagcat ccccctggcc aagaacctgc tgcaggaacc ctacaagctg accatcgtgg
    10141 gcaccgtgcg gagcaacaag cgggagatcc cagaggtgct gaagaacagc agatccagac
    10201 ctgtgggaac aagcatgttc tgcttcgacg gccccctgac cctggtgtcc tacaagccca
    10261 agcccgccaa gatggtgtac ctgctgtcca gctgcgacga ggacgccagc atcaacgaga
    10321 gcaccggcaa gccccagatg gtgatgtact acaaccagac caagggcggc gtggacaccc
    10381 tggaccagat gtgcagcgtg atgacctgca gcagaaagac caacagatgg cccatggccc
    10441 tgctgtacgg catgatcaat atcgcctgca tcaacagctt catcatctac agccacaacg
    10501 tgtccagcaa gggcgagaag gtgcagagcc ggaagaaatt catgcggaac ctgtacatga
    10561 gcctgacctc cagcttcatg agaaagagac tggaagcccc caccctgaag agatacctgc
    10621 gggacaacat cagcaacatc ctgcccaagg aagtgccagg aacaagcgac gacagcaccg
    10681 aggaacccgt gatgaagaag aggacctact gcacctactg tcccagcaag atcagaagaa
    10741 aggccaacgc cagctgcaag aaatgcaaaa aagtgatctg ccgggagcac aacatcgaca
    10801 tgtgccagag ctgtttctga catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10861 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10921 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10981 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11041 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11101 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    11161 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11221 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11281 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    11341 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11401 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11461 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11521 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatccctgtt
    11581 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
    11641 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
    11701 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
    11761 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
    11821 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
    11881 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
    11941 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
    12001 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
    12061 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
    12121 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
    12181 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
    12241 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
    12301 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
    12361 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
    12421 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
    12481 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcca
    12541 tttccccgaa aagtgccac
//
