LOCUS       sequence-373211-        9245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA targeting Cortactin + reconstitution 
            of Cortactin3YF mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Liang X, Budnar S, Gupta S, Verma S, Han SP, Hill MM, Daly RJ, 
            Parton RG, Hamilton NA, Gomez GA, Yap AS
  TITLE     Tyrosine dephosphorylated cortactin downregulates contractility at 
            the epithelial zonula adherens through SRGAP1.
  JOURNAL   Nat Commun. 2017 Oct 5;8(1):790. doi: 10.1038/s41467-017-00797-w.
  PUBMED    28983097
REFERENCE   2  (bases 1 to 9245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-5"; volume: "8(1):790. doi"; pages: " 
            10.1038/s41467-017-00797-w"
COMMENT     Sequence Label: pLL5.0 hCortKD-Flcortmut3YF EGFP
FEATURES             Location/Qualifiers
     source          1..9245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2616..2928
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2847..2867
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2985..3001
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3043..3076)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5294..5303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5300..5302,5303..5305,5306..6019)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5300..5302,5303..5305,5306..6016)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5345..5366)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5606..5625)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5953..5974
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6047..6080)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6136..6722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6189..6209)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6607..6618)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6725..6741)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7251..7431
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7493..8081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7573..7592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8252..9043,9044..9112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8875..8894
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9113..9217)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgcgct ctagagatcc gacgcgccat ctctaggccc
     2641 gcgccggccc cctcgcacag acttgtggga gaagctcggc tactcccctg ccccggttaa
     2701 tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat
     2761 ctgttctttt taatactagc tacattttac atgataggct tggatttcta taagagatac
     2821 aaatactaaa ttattatttt aaaaaacagc acaaaaggaa actcacccta actgtaaagt
     2881 aattgtgtgt tttgagacta taaatatccc ttggagaaaa gccttgtttg agaagcacga
     2941 gtcacagatt caagagatct gtgactcgtg cttctctttt ttctcgaggt cgacggtatc
     3001 gataagctcg cttcacgaga ttccagcagg tcgagggacc taataacttc gtatagcata
     3061 cattatacga agttatatta agggttccaa gcttaagcgg ccgctgaaag accccacctg
     3121 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg
     3181 agaatagaga agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca
     3241 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     3301 tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     3361 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     3421 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca
     3481 gtcctccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct cttgctgttt
     3541 gcatccgaat cgtggactcg ctgatccttg ggagggtctc ctcagattga ttgactgccc
     3601 acctcggggg tctttcatcg aattcgccac catgtggaaa gcttcagcag gccacgctgt
     3661 gtccatcgcc caggatgacg cgggggccga tgactgggag actgaccctg attttgtgaa
     3721 tgatgtgagt gagaaggagc aaagatgggg tgccaagacg gtgcagggct ccgggcacca
     3781 ggagcatatc aacatacaca agctgaggga gaatgtcttt caagagcatc agacccttaa
     3841 ggagaaggaa cttgaaacag gaccaaaagc ttcccatggc tatggaggga aatttggtgt
     3901 ggaacaagac cgaatggata agtcagctgt cggccacgaa tatcagtcga aactttccaa
     3961 gcactgctcg caggtggact cggtccgtgg cttcggaggc aagtttggtg tccagatgga
     4021 cagagttgat cagtctgctg taggctttga ataccagggg aagactgaga agcatgcctc
     4081 ccagaaagac tactccagtg gttttggcgg caagtatggc gtgcaggccg accgagtaga
     4141 caagagcgcg gtgggcttcg actaccaggg caagacggag aaacatgaat cccagagaga
     4201 ttactccaaa ggtttcggcg gcaaatacgg tatcgacaag gacaaagtgg ataagagcgc
     4261 cgttggcttt gagtatcaag gcaaaacgga gaagcacgag tcccagaaag actatgtgaa
     4321 agggtttgga ggaaaatttg gtgtgcagac agacagacaa gacaaatgtg cccttggctg
     4381 ggatcaccag gagaaattgc agctgcatga atcccaaaaa gattataaga ctggttttgg
     4441 aggcaaattc ggtgttcagt cggagaggca ggactccgct gctgtggggt ttgattacaa
     4501 ggagaagctg gccaagcacg agtcccagca agactactcc aaaggattcg gcgggaagta
     4561 tggggtgcag aaggatcgga tggataagaa tgcgtcaacc tttgaggatg tcacccaggt
     4621 gtcctctgcc taccagaaga cagtacctgt cgaagctgtg accagcaaaa caagtaacat
     4681 cagagctaac tttgaaaacc tcgctaagga gaaagagcag gaggacaggc ggaaggcgga
     4741 ggcggagaga gcccagcgga tggccaagga gcggcaggag caggaagagg ccaggaggaa
     4801 gctggaggag caagccagag ccaaaacgca aacgccccct gtgtcgcccg cacctcagcc
     4861 aaccgaggag aggctgccct cgagccccgt ctttgaggat gcggcttcct tcaaggcaga
     4921 gctgagctac agaggccctg tgagtgggac ggagccggag cccgtgtaca gcatggaggc
     4981 cgctgactac cgagaggcca gcagccagca gggcctggcc tatgccacag aggctgtctt
     5041 tgaaagcgca gaggccccgg gccactatcc cgcagaggac agcacctttg atgagtacga
     5101 gaacgatctg gggatcacag ccgtcgccct gtacgactac caggctgcgg gcgatgatga
     5161 gatctcattt gaccctgatg acatcatcac caacatcgag atgattgacg acggctggtg
     5221 gcgcggggtg tgcaagggcc ggtacgggct cttcccagcc aactatgtgg agctgcggca
     5281 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5341 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5401 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5461 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     5521 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5581 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     5641 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     5701 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     5761 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     5821 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5881 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5941 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     6001 ggacgagctg tacaagtaac ctgcaggcaa ttcgtcgagg gacctaataa cttcgtatag
     6061 catacattat acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat
     6121 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6181 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6241 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6301 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6361 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6421 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6481 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6541 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6601 gtcccttcgg ccctcaatcc agcggacctt ccttcccggc ctgctgccgg ctctgcggcc
     6661 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6721 gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc acaagtagca
     6781 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     6841 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6901 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6961 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     7021 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     7081 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     7141 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     7201 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     7261 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7321 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7381 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatgtgag
     7441 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7501 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7561 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7621 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7681 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7741 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7801 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7861 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7921 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7981 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8041 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8101 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8161 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8221 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8281 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8341 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8401 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8461 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8521 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8581 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8641 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8701 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8761 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8821 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8881 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8941 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9001 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9061 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9241 ctgac
//
