LOCUS       sequence-373225-        9271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lenticiral expression of mCherry-Cortactin W22A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Liang X, Budnar S, Gupta S, Verma S, Han SP, Hill MM, Daly RJ, 
            Parton RG, Hamilton NA, Gomez GA, Yap AS
  TITLE     Tyrosine dephosphorylated cortactin downregulates contractility at 
            the epithelial zonula adherens through SRGAP1.
  JOURNAL   Nat Commun. 2017 Oct 5;8(1):790. doi: 10.1038/s41467-017-00797-w.
  PUBMED    28983097
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-10-5"; volume: "8(1):790. doi"; pages: " 
            10.1038/s41467-017-00797-w"
COMMENT     Sequence Label: pLL5.0 CortW22A mCherry
FEATURES             Location/Qualifiers
     source          1..9271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(77..96)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        268..647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        649..847
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     802..822
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             865..1045
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1092..1217
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1714..1947
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2132..2176
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2474..2591
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2646..2958
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2877..2897
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     3015..3031
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3073..3106)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5322..5331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5473..5491)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5741..5760
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(6103..6136)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6192..6778
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6245..6265)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6663..6674)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6781..6797)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7307..7487
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7549..8137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7629..7648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8308..9099,9100..9168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8931..8950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9169..9271,1..2))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
       61 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
      121 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
      181 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      241 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      301 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      361 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      421 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      481 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      541 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      601 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      661 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      721 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      781 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      841 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
      901 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      961 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1021 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1081 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1141 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1201 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1261 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1321 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1381 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1441 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1501 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1561 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1621 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1681 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1741 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1801 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1861 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1921 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1981 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     2041 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     2101 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2161 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2221 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2281 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2341 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2401 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2461 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2521 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2581 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgcgct
     2641 ctagagatcc gacgcgccat ctctaggccc gcgccggccc cctcgcacag acttgtggga
     2701 gaagctcggc tactcccctg ccccggttaa tttgcatata atatttccta gtaactatag
     2761 aggcttaatg tgcgataaaa gacagataat ctgttctttt taatactagc tacattttac
     2821 atgataggct tggatttcta taagagatac aaatactaaa ttattatttt aaaaaacagc
     2881 acaaaaggaa actcacccta actgtaaagt aattgtgtgt tttgagacta taaatatccc
     2941 ttggagaaaa gccttgtttg agaagcacga gtcacagatt caagagatct gtgactcgtg
     3001 cttctctttt ttctcgaggt cgacggtatc gataagctcg cttcacgaga ttccagcagg
     3061 tcgagggacc taataacttc gtatagcata cattatacga agttatatta agggttccaa
     3121 gcttaagcgg ccgctgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     3181 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga
     3241 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     3301 gctcagggcc aagaacagat ggtccccaga tgcggtcgcc ctcagcagtt tctagagaac
     3361 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     3421 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     3481 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc
     3541 gtattcccaa taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg
     3601 agggtctcct cagattgatt gactgcccac ctcgggggtc tttcatcgaa ttcgccacca
     3661 tgtggaaagc ttcagcaggc cacgctgtgt ccatcgccca ggatgacgcg ggggccgatg
     3721 acgcagagac cgaccctgat tttgtgaatg atgtgagtga gaaggagcaa agatggggtg
     3781 ccaagacggt gcagggctcc gggcaccagg agcatatcaa catacacaag ctgagggaga
     3841 atgtctttca agagcatcag acccttaagg agaaggaact tgaaacagga ccaaaagctt
     3901 cccatggcta tggagggaaa tttggtgtgg aacaagaccg aatggataag tcagctgtcg
     3961 gccacgaata tcagtcgaaa ctttccaagc actgctcgca ggtggactcg gtccgtggct
     4021 tcggaggcaa gtttggtgtc cagatggaca gagttgatca gtctgctgta ggctttgaat
     4081 accaggggaa gactgagaag catgcctccc agaaagacta ctccagtggt tttggcggca
     4141 agtatggcgt gcaggccgac cgagtagaca agagcgcggt gggcttcgac taccagggca
     4201 agacggagaa acatgaatcc cagagagatt actccaaagg tttcggcggc aaatacggta
     4261 tcgacaagga caaagtggat aagagcgccg ttggctttga gtatcaaggc aaaacggaga
     4321 agcacgagtc ccagaaagac tatgtgaaag ggtttggagg aaaatttggt gtgcagacag
     4381 acagacaaga caaatgtgcc cttggctggg atcaccagga gaaattgcag ctgcatgaat
     4441 cccaaaaaga ttataagact ggttttggag gcaaattcgg tgttcagtcg gagaggcagg
     4501 actccgctgc tgtggggttt gattacaagg agaagctggc caagcacgag tcccagcaag
     4561 actactccaa aggattcggc gggaagtatg gggtgcagaa ggatcggatg gataagaatg
     4621 cgtcaacctt tgaggatgtc acccaggtgt cctctgccta ccagaagaca gtacctgtcg
     4681 aagctgtgac cagcaaaaca agtaacatca gagctaactt tgaaaacctc gctaaggaga
     4741 aagagcagga ggacaggcgg aaggcggagg cggagagagc ccagcggatg gccaaggagc
     4801 ggcaggagca ggaagaggcc aggaggaagc tggaggagca agccagagcc aaaacgcaaa
     4861 cgccccctgt gtcgcccgca cctcagccaa ccgaggagag gctgccctcg agccccgtct
     4921 atgaggatgc ggcttccttc aaggcagagc tgagctacag aggccctgtg agtgggacgg
     4981 agccggagcc cgtgtacagc atggaggccg ctgactaccg agaggccagc agccagcagg
     5041 gcctggccta tgccacagag gctgtctatg aaagcgcaga ggccccgggc cactatcccg
     5101 cagaggacag cacctacgat gagtacgaga acgatctggg gatcacagcc gtcgccctgt
     5161 acgactacca ggctgcgggc gatgatgaga tctcatttga ccctgatgac atcatcacca
     5221 acatcgagat gattgacgac ggctggtggc gcggggtgtg caagggccgg tacgggctct
     5281 tcccagccaa ctatgtggag ctgcggcagg atccaccggt cgccaccatg gtgagcatgg
     5341 acgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     5401 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     5461 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     5521 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     5581 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     5641 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     5701 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     5761 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     5821 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     5881 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     5941 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     6001 gccactccac cggcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat
     6061 aatcagccat accaccctgc aggcaattcg tcgagggacc taataacttc gtatagcata
     6121 cattatacga agttatacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca
     6181 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6241 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6301 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6361 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6421 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6481 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6541 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6601 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6661 cttcggccct caatccagcg gaccttcctt cccggcctgc tgccggctct gcggcctctt
     6721 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6781 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6841 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6901 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6961 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7021 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     7081 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     7141 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     7201 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     7261 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     7321 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7381 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7441 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa
     7501 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7561 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7621 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7681 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7741 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7801 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7861 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7921 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7981 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8041 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8101 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8161 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8221 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8281 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8341 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8401 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8461 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8521 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8581 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8641 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8701 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8761 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8821 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8881 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8941 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9001 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9061 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9121 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9181 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9241 tatttagaaa aataaacaaa taggggttcc g
//
