LOCUS       sequence-373226-       11977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full-length human p300 with DY1399 mutation,
            acetylation mutant, acetyltransferase activity defect.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Askew EB, Bai S, Blackwelder AJ, Wilson EM
  TITLE     Transcriptional synergy between melanoma antigen gene protein-A11 
            (MAGE-11) and p300 in androgen receptor signaling.
  JOURNAL   J Biol Chem. 2010 Jul 9;285(28):21824-36. doi: 
            10.1074/jbc.M110.120600. Epub 2010 May 6.
  PUBMED    20448036
REFERENCE   2  (bases 1 to 11977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.120600"; journalName: "J Biol Chem"; date: 
            "2010-07-9- 9"; volume: "285"; issue: "28"; pages: "21824-36"
COMMENT     Sequence Label: pSG5-HA-p300-DY1399
FEATURES             Location/Qualifiers
     source          1..11977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        23..219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          250..822
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     830..849
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     877..896
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     915..931
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     915..931
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             942..968
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4110..5960
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300, inactivated by the 
                     D1399Y point mutation (Hilton et al., 2015)"
                     /label=p300 core (D1399Y)
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLYSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GNFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      5724..5733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8839..8960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8876..8895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8930..8949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8994..9011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9165..9753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9245..9264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9924..10715,10716..10784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10547..10566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10785..10889)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10957..10975
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11013..11035)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11135..11154
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(11221..11676)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11358..11379)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11570..11589
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11802..11824
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11816..11833
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11817..11833
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11870..11890)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      181 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctccggat cgatcctgag
      241 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      301 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      361 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      421 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      481 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      541 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      601 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      661 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
      721 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
      781 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      841 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
      901 ttcggatcgg ccgctctaga actagtggat ccaccatggc atacccatac gacgtgcctg
      961 actacgcctc cgccgagaat gtggtggaac cggggccgcc ttcagccaag cggcctaaac
     1021 tctcatctcc ggccctctcg gcgtccgcca gcgatggcac agattttggc tctctatttg
     1081 acttggagca cgacttacca gatgaattaa tcaactctac agaattggga ctaaccaatg
     1141 gtggtgatat taatcagctt cagacaagtc ttggcatggt acaagatgca gcttctaaac
     1201 ataaacagct gtcagaattg ctgcgatctg gtagttcccc taacctcaat atgggagttg
     1261 gtggcccagg tcaagtcatg gccagccagg cccaacagag cagtcctgga ttaggtttga
     1321 taaatagcat ggtcaaaagc ccaatgacac aggcaggctt gacttctccc aacatgggga
     1381 tgggcactag tggaccaaat cagggtccta cgcagtcaac aggtatgatg aacagtccag
     1441 taaatcagcc tgccatggga atgaacacag ggatgaatgc gggcatgaat cctggaatgt
     1501 tggctgcagg caatggacaa gggataatgc ctaatcaagt catgaacggt tcaattggag
     1561 caggccgagg gcgacagaat atgcagtacc caaacccagg catgggaagt gctggcaact
     1621 tactgactga gcctcttcag cagggctctc cccagatggg aggacaaaca ggattgagag
     1681 gcccccagcc tcttaagatg ggaatgatga acaaccccaa tccttatggt tcaccatata
     1741 ctcagaatcc tggacagcag attggagcca gtggccttgg tctccagatt cagacaaaaa
     1801 ctgtactatc aaataactta tctccatttg ctatggacaa aaaggcagtt cctggtggag
     1861 gaatgcccaa catgggtcaa cagccagccc cgcaggtcca gcagccaggt ctggtgactc
     1921 cagttgccca agggatgggt tctggagcac atacagctga tccagagaag cgcaagctca
     1981 tccagcagca gcttgttctc cttttgcatg ctcacaagtg ccagcgccgg gaacaggcca
     2041 atggggaagt gaggcagtgc aaccttcccc actgtcgcac aatgaagaat gtcctaaacc
     2101 acatgacaca ctgccagtca ggcaagtctt gccaagtggc acactgtgca tcttctcgac
     2161 aaatcatttc acactggaag aattgtacaa gacatgattg tcctgtgtgt ctccccctca
     2221 aaaatgctgg tgataagaga aatcaacagc caattttgac tggagcaccc gttggacttg
     2281 gaaatcctag ctctctaggg gtgggtcaac agtctgcccc caacctaagc actgttagtc
     2341 agattgatcc cagctccata gaaagagcct atgcagctct tggactaccc tatcaagtaa
     2401 atcagatgcc gacacaaccc caggtgcaag caaagaacca gcagaatcag cagcctgggc
     2461 agtctcccca aggcatgcgg cccatgagca acatgagtgc tagtcctatg ggagtaaatg
     2521 gaggtgtagg agttcaaacg ccgagtcttc tttctgactc aatgttgcat tcagccataa
     2581 attctcaaaa cccaatgatg agtgaaaatg ccagtgtgcc ctccctgggt cctatgccaa
     2641 cagcagctca accatccact actggaattc ggaaacagtg gcacgaagat attactcagg
     2701 atcttcgaaa tcatcttgtt cacaaactcg tccaagccat atttcctacg ccggatcctg
     2761 ctgctttaaa agacagacgg atggaaaacc tagttgcata tgctcggaaa gttgaagggg
     2821 acatgtatga atctgcaaac aatcgagcgg aatactacca ccttctagct gagaaaatct
     2881 ataagatcca gaaagaacta gaagaaaaac gaaggaccag actacagaag cagaacatgc
     2941 taccaaatgc tgcaggcatg gttccagttt ccatgaatcc agggcctaac atgggacagc
     3001 cgcaaccagg aatgacttct aatggccctc tacctgaccc aagtatgatc cgtggcagtg
     3061 tgccaaacca gatgatgcct cgaataactc cacaatctgg tttgaatcaa tttggccaga
     3121 tgagcatggc ccagccccct attgtacccc ggcaaacccc tcctcttcag caccatggac
     3181 agttggctca acctggagct ctcaacccgc ctatgggcta tgggcctcgt atgcaacagc
     3241 cttccaacca gggccagttc cttcctcaga ctcagttccc atcacaggga atgaatgtaa
     3301 caaatatccc tttggctccg tccagcggtc aagctccagt gtctcaagca caaatgtcta
     3361 gttcttcctg cccggtgaac tctcctataa tgcctccagg gtctcagggg agccacattc
     3421 actgtcccca gcttcctcaa ccagctcttc atcagaattc accctcgcct gtacctagtc
     3481 gtacccccac ccctcaccat actcccccaa gcataggggc tcagcagcca ccagcaacaa
     3541 caattccagc ccctgttcct acacctcctg ccatgccacc tgggccacag tcccaggctc
     3601 tacatccccc tccaaggcag acacctacac caccaacaac acaacttccc caacaagtgc
     3661 agccttcact tcctgctgca ccttctgctg accagcccca gcagcagcct cgctcacagc
     3721 agagcacagc agcgtctgtt cctaccccaa cagcaccgct gcttcctccg cagcctgcaa
     3781 ctccactttc ccagccagct gtaagcattg aaggacaggt atcaaatcct ccatctacta
     3841 gtagcacaga agtgaattct caggccattg ctgagaagca gccttcccag gaagtgaaga
     3901 tggaggccaa aatggaagtg gatcaaccag aaccagcaga tactcagccg gaggatattt
     3961 cagagtctaa agtggaagac tgtaaaatgg aatctaccga aacagaagag agaagcactg
     4021 agttaaaaac tgaaataaaa gaggaggaag accagccaag tacttcagct acccagtcat
     4081 ctccggctcc aggacagtca aagaaaaaga ttttcaaacc agaagaacta cgacaggcac
     4141 tgatgccaac attggaggca ctttaccgtc aggatccaga atcccttccc tttcgtcaac
     4201 ctgtggaccc tcagctttta ggaatccctg attactttga tattgtgaag agccccatgg
     4261 atctttctac cattaagagg aagttagaca ctggacagta tcaggagccc tggcagtatg
     4321 tcgatgatat ttggcttatg ttcaataatg cctggttata taaccggaaa acatcacggg
     4381 tatacaaata ctgctccaag ctctctgagg tctttgaaca agaaattgac ccagtgatgc
     4441 aaagccttgg atactgttgt ggcagaaagt tggagttctc tccacagaca ctgtgttgct
     4501 acggcaaaca gttgtgcaca atacctcgtg atgccactta ttacagttac cagaacaggt
     4561 atcatttctg tgagaagtgt ttcaatgaga tccaagggga gagcgtttct ttgggggatg
     4621 acccttccca gcctcaaact acaataaata aagaacaatt ttccaagaga aaaaatgaca
     4681 cactggatcc tgaactgttt gttgaatgta cagagtgcgg aagaaagatg catcagatct
     4741 gtgtccttca ccatgagatc atctggcctg ctggattcgt ctgtgatggc tgtttaaaga
     4801 aaagtgcacg aactaggaaa gaaaataagt tttctgctaa aaggttgcca tctaccagac
     4861 ttggcacctt tctagagaat cgtgtgaatg actttctgag gcgacagaat caccctgagt
     4921 caggagaggt cactgttaga gtagttcatg cttctgacaa aaccgtggaa gtaaaaccag
     4981 gcatgaaagc aaggtttgtg gacagtggag agatggcaga atcctttcca taccgaacca
     5041 aagccctctt tgcctttgaa gaaattgatg gtgttgacct gtgcttcttt ggcatgcatg
     5101 ttcaagagta tggctctgac tgccctccac ccaaccagag gagagtatac atatcttacc
     5161 tctatagtgt tcatttcttc cgtcctaaat gcttgaggac tgcagtctat catgaaatcc
     5221 taattggata tttagaatat gtcaagaaat taggttacac aacagggcat atttgggcat
     5281 gtccaccaag tgagggagat gattatatct tccattgcca tcctcctgac cagaagatac
     5341 ccaagcccaa gcgactgcag gaatggtaca aaaaaatgct tgacaaggct gtatcagagc
     5401 gtattgtcca tgactacaag gatattttta aacaagctac tgaagataga ttaacaagtg
     5461 caaaggaatt gccttatttc gagggtaatt tctggcccaa tgttctggaa gaaagcatta
     5521 aggaactgga acaggaggaa gaagagagaa aacgagagga aaacaccagc aatgaaagca
     5581 cagatgtgac caagggagac agcaaaaatg ctaaaaagaa gaataataag aaaaccagca
     5641 aaaataagag cagcctgagt aggggcaaca agaagaaacc cgggatgccc aatgtatcta
     5701 acgacctctc acagaaacta tatgccacca tggagaagca taaagaggtc ttctttgtga
     5761 tccgcctcat tgctggccct gctgccaact ccctgcctcc cattgttgat cctgatcctc
     5821 tcatcccctg cgatctgatg gatggtcggg atgcgtttct cacgctggca agggacaagc
     5881 acctggagtt ctcttcactc cgaagagccc agtggtccac catgtgcatg ctggtggagc
     5941 tgcacacgca gagccaggac cgctttgtct acacctgcaa tgaatgcaag caccatgtgg
     6001 agacacgctg gcactgtact gtctgtgagg attatgactt gtgtatcacc tgctataaca
     6061 ctaaaaacca tgaccacaaa atggagaaac taggccttgg cttagatgat gagagcaaca
     6121 accagcaggc tgcagccacc cagagcccag gcgattctcg ccgcctgagt atccagcgct
     6181 gcatccagtc tctggtccat gcttgccagt gtcggaatgc caattgctca ctgccatcct
     6241 gccagaagat gaagcgggtt gtgcagcata ccaagggttg caaacggaaa accaatggcg
     6301 ggtgccccat ctgcaagcag ctcattgccc tctgctgcta ccatgccaag cactgccagg
     6361 agaacaaatg cccggtgccg ttctgcctaa acatcaagca gaagctccgg cagcaacagc
     6421 tgcagcaccg actacagcag gcccaaatgc ttcgcaggag gatggccagc atgcagcgga
     6481 ctggtgtggt tgggcagcaa cagggcctcc cttcccccac tcctgccact ccaacgacac
     6541 caactggcca acagccaacc accccgcaga cgccccagcc cacttctcag cctcagccta
     6601 cccctcccaa tagcatgcca ccctacttgc ccaggactca agctgctggc cctgtgtccc
     6661 agggtaaggc agcaggccag gtgacccctc caacccctcc tcagactgct cagccacccc
     6721 ttccagggcc cccacctgca gcagtggaaa tggcaatgca gattcagaga gcagcggaga
     6781 cgcagcgcca gatggcccac gtgcaaattt ttcaaaggcc aatccaacac cagatgcccc
     6841 cgatgactcc catggccccc atgggtatga acccacctcc catgaccaga ggtcccagtg
     6901 ggcatttgga gccagggatg ggaccgacag ggatgcagca acagccaccc tggagccaag
     6961 gaggattgcc tcagccccag caactacagt ctgggatgcc aaggccagcc atgatgtcag
     7021 tggcccagca tggtcaacct ttgaacatgg ctccacaacc aggattgggc caggtaggta
     7081 tcagcccact caaaccaggc actgtgtctc aacaagcctt acaaaacctt ttgcggactc
     7141 tcaggtctcc cagctctccc ctgcagcagc aacaggtgct tagtatcctt cacgccaacc
     7201 cccagctgtt ggctgcattc atcaagcagc gggctgccaa gtatgccaac tctaatccac
     7261 aacccatccc tgggcagcct ggcatgcccc aggggcagcc agggctacag ccacctacca
     7321 tgccaggtca gcagggggtc cactccaatc cagccatgca gaacatgaat ccaatgcagg
     7381 cgggcgttca gagggctggc ctgccccagc agcaaccaca gcagcaactc cagccaccca
     7441 tgggagggat gagcccccag gctcagcaga tgaacatgaa ccacaacacc atgccttcac
     7501 aattccgaga catcttgaga cgacagcaaa tgatgcaaca gcagcagcaa cagggagcag
     7561 ggccaggaat aggccctgga atggccaacc ataaccagtt ccagcaaccc caaggagttg
     7621 gctacccacc acagcagcag cagcggatgc agcatcacat gcaacagatg caacaaggaa
     7681 atatgggaca gataggccag cttccccagg ccttgggagc agaggcaggt gccagtctac
     7741 aggcctatca gcagcgactc cttcagcaac agatggggtc ccctgttcag cccaacccca
     7801 tgagccccca gcagcatatg ctcccaaatc aggcccagtc cccacaccta caaggccagc
     7861 agatccctaa ttctctctcc aatcaagtgc gctctcccca gcctgtccct tctccacggc
     7921 cacagtccca gcccccccac tccagtcctt ccccaaggat gcagcctcag ccttctccac
     7981 accacgtttc cccacagaca agttccccac atcctggact ggtagctgcc caggccaacc
     8041 ccatggaaca agggcatttt gccagcccgg accagaattc aatgctttct cagcttgcta
     8101 gcaatccagg catggcaaac ctccatggtg caagcgccac ggacctggga ctcagcaccg
     8161 ataactcaga cttgaattca aacctctcac agagtacact agacatacac tagagacacc
     8221 ttgtagtatt ttgggagcaa aaaaattatt ttctcttaac aagacttttt gtactgaaaa
     8281 caattttttt gaatctttcg tagcctaaaa gacaattttc cttggaacac ataagaactg
     8341 tgcagtagcc gtttgtggtt taaagcaaac atgcaagatg aacctgaggg atgatagaat
     8401 acaaagaata tatttttgtt atggctggtt accaccagcc tttcttcccc tttgtgtgtg
     8461 tggttcaagt gtgcactggg aggaggctga ggcctgtgaa gccaaacaat atgctcctgc
     8521 cttgcacctc caataggttt tattattttt tttaaattaa tgaacatatg taatattaat
     8581 agttattatt tactggtgca gatggttgac atttttccct attttcctca ctttatggaa
     8641 gagttaaaac atttctaaac cagaggacaa aaggggttaa tgttacttta aaattacatt
     8701 ctatatatat ataaatatat ataaatatat attaaaatac cagttttttt tctctgggtg
     8761 caaagatgtt cattctttta aaaaatgttt aaaaaaaagg aattcgatat caagctgatc
     8821 cagatcttat taaagcagaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8881 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8941 aaactcatca atgtatctta tcatgtctgg tcgactctag actcttccgc ttcctcgctc
     9001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9361 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9601 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9721 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9781 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9841 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9901 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9961 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10021 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10081 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10141 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10201 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10921 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    10981 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    11041 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    11101 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    11161 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    11221 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
    11281 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
    11341 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
    11401 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
    11461 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
    11521 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
    11581 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
    11641 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
    11701 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    11761 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    11821 aacgacggcc agtgaattgt cgacttctga ggcggaaaga accagctgtg gaatgtgtgt
    11881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    11941 ctcaattagt cagcaaggaa agtccccagg ctcccca
//
