LOCUS       sequence-373241-       10907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA targeting Anillin + reconstitution of
            Anillin full length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, 
            Hamilton NA, Morris RG, Yap AS
  TITLE     Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA 
            Residence Kinetics
  JOURNAL   Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 
            10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
  PUBMED    31105010
REFERENCE   2  (bases 1 to 10907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.04.031"; journalName: "Dev Cell"; date: 
            "2019-06-17- 17"; volume: "49"; issue: "6"; pages: "894-906.e12"
COMMENT     Sequence Label: PLL5.0-Anillin ShRNA-Cherry-AnillinFL
FEATURES             Location/Qualifiers
     source          1..10907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(67..86)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        258..637
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        639..837
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             855..1035
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1082..1207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1704..1937
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2122..2166
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2464..2581
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2636..2948
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2867..2887
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     3011..3027
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3069..3102)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        3182..3485
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3486..3689
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3718..3727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3724..4431
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3869..3887)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4137..4156
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(7729..7762)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7818..8404
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7871..7891)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8289..8300)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8407..8423)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8933..9113
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9175..9763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9255..9274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9934..10725,10726..10794))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10557..10576
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10795..10899)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
       61 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
      121 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
      181 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
      241 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
      301 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      361 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      421 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      481 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      541 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      601 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      661 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      721 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      781 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
      841 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     1021 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     1081 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     1141 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     1201 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     1261 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     1321 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1381 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1441 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1501 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
     1561 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1621 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1681 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1741 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1801 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1861 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1921 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1981 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     2041 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     2101 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     2161 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     2221 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     2281 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     2341 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     2401 ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca
     2461 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2521 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2581 tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgcgct ctagagatcc
     2641 gacgcgccat ctctaggccc gcgccggccc cctcgcacag acttgtggga gaagctcggc
     2701 tactcccctg ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg
     2761 tgcgataaaa gacagataat ctgttctttt taatactagc tacattttac atgataggct
     2821 tggatttcta taagagatac aaatactaaa ttattatttt aaaaaacagc acaaaaggaa
     2881 actcacccta actgtaaagt aattgtgtgt tttgagacta taaatatccc ttggagaaaa
     2941 gccttgtttc ggcgatgcct ctttgaataa attcaagaga tttattcaaa gaggcatcgc
     3001 cattttttct cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga
     3061 gggacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt
     3121 aagcggccgc atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3181 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3241 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3301 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3361 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3421 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3481 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     3541 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     3601 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     3661 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgcc
     3721 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     3781 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     3841 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     3901 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     3961 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     4021 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     4081 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     4141 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     4201 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     4261 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     4321 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     4381 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gccgcggatg
     4441 gatccgttta cggagaaact gctggagcga acccgtgcca ggcgagagaa tcttcagaga
     4501 aaaatggctg agaggcccac agcagctcca aggtctatga ctcatgctaa gcgagctaga
     4561 cagccacttt cagaagcaag taaccagcag cccctctctg gtggtgaaga gaaatcttgt
     4621 acaaaaccat cgccatcaaa aaaacgctgt tctgacaaca ctgaagtaga agtttctaac
     4681 ttggaaaata aacaaccagt tgagtcgaca tctgcaaaat cttgttctcc aagtcctgtg
     4741 tctcctcagg tgcagccaca agcagcagat accatcagtg attctgttgc tgtcccggca
     4801 tcactgctgg gcatgaggag agggctgaac tcaagattgg aagcaactgc agcctcctca
     4861 gttaaaacac gtatgcaaaa acttgcagag caacggcgcc gttgggataa tgatgatatg
     4921 acagatgaca ttcctgaaag ctcactcttc tcaccaatgc catcagagga aaaggctgct
     4981 tcccctccca gacctctgct ttcaaatgcc tcggcaactc cagttggcag aaggggccgt
     5041 ctggccaatc ttgctgcaac tatttgctcc tgggaagatg atgtaaatca ctcatttgca
     5101 aaacaaaaca gtgtacaaga acagcctggt accgcttgtt tatccaaatt ttcctctgca
     5161 agtggagcat ctgctaggat caatagcagc agtgttaagc aggaagctac attctgttcc
     5221 caaagggatg gcgacgcaag cttaaacaag gccctatcct caagtgctgc tgatgcgtct
     5281 ttggttaatg cctcaatttc cagctctgtg aaagctactt ctccagtgaa atctactaca
     5341 tctatcactg atgctaaaag ttgtgaggga caaaatcctg agctacttcc aaaaactcct
     5401 attagtcctc tgaaaacggg ggtatcgaaa ccaattgtga agtcaacttt atcccagaca
     5461 gttccatcca agggagaatt aagtagagaa atttgtctgc aatctcaatc taaagacaaa
     5521 tctacgacac caggaggaac aggaattaag cctttcctgg aacgctttgg agagcgttgt
     5581 caagaacata gcaaagaaag tccagctcgt agcacacccc acagaacccc cattattact
     5641 ccaaatacaa aggccatcca agaaaaatta ttcaagcaag acacatcttc atctactacc
     5701 catttagcac aacagctcaa gcaggaacgt caaaaagaac tagcatgtct tcgtggccga
     5761 tttgacaagg gcaatatatg gagtgcagaa aaaggcggaa actcaaaaag caaacaacta
     5821 gaaaccaaac aggaaactca ctgtcagagc actcccctca aaaaacacca aggtgtttca
     5881 aaaactcagt cacttccagt aacagaaaag gtgaccgaaa accagatacc agccaaaaat
     5941 tctagtacag aacctaaaga agtgatacgt gaaattgaga tgagtgtgga tgatgatgat
     6001 atcaatagtt cgaaagtaat taatgacctc ttcagtgatg tcctagagga aggtgaacta
     6061 gatatggaga agagccaaga ggagatggat caagcattag cagaaagcag cgaagaacag
     6121 gaagatgcac tgaatatctc ctcaatgtct ttacttgcac cattggcaca aacagttggt
     6181 gtggtaagtc cagagagttt agtgtccaca cctagactgg aattgaaaga caccagcaga
     6241 agtgatgaaa gtccaaaacc aggaaaattc caaagaactc gtgtccctcg agctgaatct
     6301 ggtgatagcc ttggttctga agatcgtgat cttctttaca gcattgatgc atatagatct
     6361 caaagattca aagaaacaga acgtccatca ataaagcagg tgattgttcg gaaggaagat
     6421 gttacttcaa aactggatga aaaaaataat gcctttcctt gtcaagttaa tatcaaacag
     6481 aaaatgcagg aactcaataa cgaaataaat atgcaacaga cagtgatcta tcaagctagc
     6541 caggctctta actgctgtgt tgatgaagaa catggaaaag ggtccctaga agaagctgaa
     6601 gcagaaagac ttcttctaat tgcaactggg aagagaacac ttttgattga tgaattgaat
     6661 aaattgaaga acgaaggacc tcagaggaag aataaggcta gtccccaaag tgaatttatg
     6721 ccatccaaag gatcagttac tttgtcagaa atccgcttgc ctctaaaagc agattttgtc
     6781 tgcagtacgg ttcagaaacc agatgcagca aattactatt acttaattat actaaaagca
     6841 ggagctgaaa atatggtagc cacaccatta gcaagtactt caaactctct taacggtgat
     6901 gctctgacat tcactactac atttactctg caagatgtat ccaatgactt tgaaataaat
     6961 attgaagttt acagcttggt gcaaaagaaa gatccctcag gccttgataa gaagaaaaaa
     7021 acatccaagt ccaaggctat tactccaaag cgactcctca catctataac cacaaaaagc
     7081 aacattcatt cttcagtcat ggccagtcca ggaggtctta gtgctgtgcg aaccagcaac
     7141 ttcgcccttg ttggatctta cacattatca ttgtcttcag taggaaatac taagtttgtt
     7201 ctggacaagg tccccttttt atcttctttg gaaggtcata tttatttaaa aataaaatgt
     7261 caagtgaatt ccagtgttga agaaagaggt tttctaacca tatttgaaga tgttagtggt
     7321 tttggtgcct ggcatcgaag atggtgtgtt ctttctggaa actgtatatc ttattggact
     7381 tatccagatg atgagaaacg caagaatccc ataggaagga taaatctggc taattgtacc
     7441 agtcgtcaga tagaaccagc caacagagaa ttttgtgcaa gacgcaacac ttttgaatta
     7501 attactgtcc gaccacaaag agaagatgac cgagagactc ttgtcagcca atgcagggac
     7561 acactctgtg ttaccaagaa ctggctgtct gcagatacta aagaagagcg ggatctctgg
     7621 atgcaaaaac tcaatcaagt tcttgttgat attcgcctct ggcaacctga tgcttgctac
     7681 aaacctattg gaaagcctta acctgcaggc aattcgtcga gggacctaat aacttcgtat
     7741 agcatacatt atacgaagtt atacatgttt aagggttccg gttccactag gtacaattcg
     7801 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7861 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7921 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7981 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     8041 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8101 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8161 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     8221 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8281 acgtcccttc ggccctcaat ccagcggacc ttccttcccg gcctgctgcc ggctctgcgg
     8341 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8401 ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag
     8461 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     8521 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     8581 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8641 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     8701 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     8761 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     8821 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     8881 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     8941 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9001 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9061 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg
     9121 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9181 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9241 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9361 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9601 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9721 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9781 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9841 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9901 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9961 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10021 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10081 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10141 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10201 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10261 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10321 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10381 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10441 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10501 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10561 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10621 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10681 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10741 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10801 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10861 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttc
//
