LOCUS       sequence-373245-       10907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA targeting Anillin + reconstitution of
            Anillin A740D,E758K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, 
            Hamilton NA, Morris RG, Yap AS
  TITLE     Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA 
            Residence Kinetics
  JOURNAL   Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 
            10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
  PUBMED    31105010
REFERENCE   2  (bases 1 to 10907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.04.031"; journalName: "Dev Cell"; date: 
            "2019-06-17- 17"; volume: "49"; issue: "6"; pages: "894-906.e12"
COMMENT     Sequence Label: PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K
FEATURES             Location/Qualifiers
     source          1..10907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             634..814
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(832..1030)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1032..1411
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1583..1602
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1677..1781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1782..1850,1851..2642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2000..2019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2813..3401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3302..3321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3463..3643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4153..4169
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4172..4758
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4276..4287
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4685..4705
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    4814..4847
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(8145..8852)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8420..8439)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8689..8707
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      8849..8858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8887..9090)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        9091..9394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    9474..9507
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(9549..9565)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(9628..9940)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(9689..9709)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_feature    9995..10112
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10410..10454)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10639..10872
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata
       61 taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg
      121 gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct
      181 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta
      241 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt
      301 tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc
      361 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga
      421 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct
      481 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg
      541 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc
      601 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa
      661 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
      721 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
      781 cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaacgc gctgcttata
      841 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      901 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      961 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
     1021 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     1081 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     1141 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     1201 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     1261 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     1321 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     1381 tactattaat aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga
     1441 agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc
     1501 cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg
     1561 agcagatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatagg
     1621 ggatcgggag atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg
     1681 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1741 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1801 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1861 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1921 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1981 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2041 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2101 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2161 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2221 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2281 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2341 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2401 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2461 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2521 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2581 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2641 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2701 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2761 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2821 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2881 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2941 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3001 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3061 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3121 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3181 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3241 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3301 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3361 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3421 ttttacggtt cctggccttt tgctggcctt ttgctcacat gctgctagag attttccaca
     3481 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     3541 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     3601 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag tccggatgca
     3661 gctctcgggc catgtgatga aatgctaggc ggctgtcaaa cctccactct aatacttctc
     3721 tctccgggtc atccatccca tgcaggctca cagggtgtaa caagcgggtg ttctctcctt
     3781 cattggcttc ttctaccttc tcttgctcaa ctggtactag cttgtagcac catccaaagg
     3841 tcagtggata tctgatccct ggccctggtg tgtagttctg ccaatcaggg aagtagcctt
     3901 gtgtgtggta gatccacaga tcaaggatat cttgtcttcg ttgggagtga attagccctt
     3961 ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg
     4021 gtcttaaagg tacctgaggt gtgactggaa aacccacctc ctcctcctct tgtgcttcta
     4081 gccaggcaca atcagcattg gtagctgctg tattgctact tgtgattgct ccatgttttt
     4141 ctaggtctcg atcgaggtcg acggtatcga tgcggggagg cggcccaaag ggagatccga
     4201 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccggga
     4261 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     4321 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     4381 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     4441 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     4501 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     4561 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     4621 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     4681 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     4741 gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt ggaaccggaa
     4801 cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag gtccctcgac
     4861 gaattgcctg caggttaagg ctttccaata ggtttgtagc aagcatcagg ttgccagagg
     4921 cgaatatcaa caagaacttg attgagtttt tgcatccaga gatcccgctc ttctttagta
     4981 tctgcagaca gccagttctt ggtaacacag agtgtgtccc tgcattggct gacaagagtc
     5041 tctcggtcat cttctctttg tggtcggaca gtaattaatt caaaagtgtt gcgtcttgca
     5101 caaaattctc tgttggctgg ttctatctga cgactggtac aattagccag atttatcctt
     5161 cctatgggat tcttgcgttt ctcatcatct ggataagtcc aataagatat acagtttcca
     5221 gaaagaacac accatcttcg atgccaggca ccaaaaccac taacatcttc aaatatggtt
     5281 agaaaacctc tttcttcaac actggaattc acttgacatt ttatttttaa ataaatatga
     5341 ccttccaaag aagataaaaa ggggaccttg tccagaacaa acttagtatt tcctactgaa
     5401 gacaatgata atgtgtaaga tccaacaagg gcgaagttgc tggttcgcac agcactaaga
     5461 cctcctggac tggccatgac tgaagaatga atgttgcttt ttgtggttat agatgtgagg
     5521 agtcgctttg gagtaatagc cttggacttg gatgtttttt tcttcttatc aaggcctgag
     5581 ggatctttct tttgcaccaa gctgtaaact tcaatattta tttcaaagtc attggataca
     5641 tcttgcagag taaatgtagt agtgaatgtc agagcatcac cgttaagaga gtttgaagta
     5701 cttgctaatg gtgtggctac catattttca gctcctgctt ttagtataat taagtaatag
     5761 taatttgctg catctggttt ctgaaccgta ctgcagacaa aatctgcttt tagaggcaag
     5821 cggatttctg acaaagtaac tgatcctttg gatggcataa attcactttg gggactagcc
     5881 ttattcttcc tctgaggtcc ttcgttcttc aatttattca attcatcaat caaaagtgtt
     5941 ctcttcccag ttgcaattag aagaagtctt tctgctttag cttcttctag ggaccctttt
     6001 ccatgttctt catcaacaca gcagttaaga tcctggctag cttgatagat cactgtctgt
     6061 tgcatattta tttcgttatt gagttcctgc attttctgtt tgatattaac ttgacaagga
     6121 aaggcattat ttttttcatc cagttttgaa gtaacatctt ccttccgaac aatcacctgc
     6181 tttattgatg gacgttctgt ttctttgaat ctttgagatc tatatgcatc aatgctgtaa
     6241 agaagatcac gatcttcaga accaaggcta tcaccagatt cagctcgagg gacacgagtt
     6301 ctttggaatt ttcctggttt tggactttca tcacttctgc tggtgtcttt caattccagt
     6361 ctaggtgtgg acactaaact ctctggactt accacaccaa ctgtttgtgc caatggtgca
     6421 agtaaagaca ttgaggagat attcagtgca tcttcctgtt cttcgctgct ttctgctaat
     6481 gcttgatcca tctcctcttg gctcttctcc atatctagtt caccttcctc taggacatca
     6541 ctgaagaggt cattaattac tttcgaacta ttgatatcat catcatccac actcatctca
     6601 atttcacgta tcacttcttt aggttctgta ctagaatttt tggctggtat ctggttttcg
     6661 gtcacctttt ctgttactgg aagtgactga gtttttgaaa caccttggtg ttttttgagg
     6721 ggagtgctct gacagtgagt ttcctgtttg gtttctagtt gtttgctttt tgagtttccg
     6781 cctttttctg cactccatat attgcccttg tcaaatcggc cacgaagaca tgctagttct
     6841 ttttgacgtt cctgcttgag ctgttgtgct aaatgggtag tagatgaaga tgtgtcttgc
     6901 ttgaataatt tttcttggat ggcctttgta tttggagtaa taatgggggt tctgtggggt
     6961 gtgctacgag ctggactttc tttgctatgt tcttgacaac gctctccaaa gcgttccagg
     7021 aaaggcttaa ttcctgttcc tcctggtgtc gtagatttgt ctttagattg agattgcaga
     7081 caaatttctc tacttaattc tcccttggat ggaactgtct gggataaagt tgacttcaca
     7141 attggtttcg atacccccgt tttcagagga ctaataggag tttttggaag tagctcagga
     7201 ttttgtccct cacaactttt agcatcagtg atagatgtag tagatttcac tggagaagta
     7261 gctttcacag agctggaaat tgaggcatta accaaagacg catcagcagc acttgaggat
     7321 agggccttgt ttaagcttgc gtcgccatcc ctttgggaac agaatgtagc ttcctgctta
     7381 acactgctgc tattgatcct agcagatgct ccacttgcag aggaaaattt ggataaacaa
     7441 gcggtaccag gctgttcttg tacactgttt tgttttgcaa atgagtgatt tacatcatct
     7501 tcccaggagc aaatagttgc agcaagattg gccagacggc cccttctgcc aactggagtt
     7561 gccgaggcat ttgaaagcag aggtctggga ggggaagcag ccttttcctc tgatggcatt
     7621 ggtgagaaga gtgagctttc aggaatgtca tctgtcatat catcattatc ccaacggcgc
     7681 cgttgctctg caagtttttg catacgtgtt ttaactgagg aggctgcagt tgcttccaat
     7741 cttgagttca gccctctcct catgcccagc agtgatgccg ggacagcaac agaatcactg
     7801 atggtatctg ctgcttgtgg ctgcacctga ggagacacag gacttggaga acaagatttt
     7861 gcagatgtcg actcaactgg ttgtttattt tccaagttag aaacttctac ttcagtgttg
     7921 tcagaacagc gtttttttga tggcgatggt tttgtacaag atttctcttc accaccagag
     7981 aggggctgct ggttacttgc ttctgaaagt ggctgtctag ctcgcttagc atgagtcata
     8041 gaccttggag ctgctgtggg cctctcagcc atttttctct gaagattctc tcgcctggca
     8101 cgggttcgct ccagcagttt ctccgtaaac ggatccatcc gcggcttgta cagctcgtcc
     8161 atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc
     8221 tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg
     8281 ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc
     8341 ttcagcctct gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag
     8401 gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg
     8461 ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg
     8521 gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag
     8581 gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac
     8641 atgaactgag gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc
     8701 agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac
     8761 tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg
     8821 gccatgttat cctcctcgcc cttgctcacc atggtggcgc tagcggatct gacggttcac
     8881 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     8941 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     9001 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     9061 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     9121 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     9181 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     9241 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     9301 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     9361 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     9421 taatgacccc gtaattgatt actatgcggc cgcttaagct tggaaccctt aatataactt
     9481 cgtataatgt atgctatacg aagttattag gtccctcgac ctgctggaat ctcgtgaagc
     9541 gagcttatcg ataccgtcga cctcgagaaa aaatggcgat gcctctttga ataaatctct
     9601 tgaatttatt caaagaggca tcgccgaaac aaggcttttc tccaagggat atttatagtc
     9661 tcaaaacaca caattacttt acagttaggg tgagtttcct tttgtgctgt tttttaaaat
     9721 aataatttag tatttgtatc tcttatagaa atccaagcct atcatgtaaa atgtagctag
     9781 tattaaaaag aacagattat ctgtctttta tcgcacatta agcctctata gttactagga
     9841 aatattatat gcaaattaac cggggcaggg gagtagccga gcttctccca caagtctgtg
     9901 cgagggggcc ggcgcgggcc tagagatggc gcgtcggatc tctagagcgc ggtactaacc
     9961 aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt
    10021 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
    10081 tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtctgc
    10141 agaattggcg cacgcagtgc cgatccgttc actaatcgaa tggatctgtc tctgtctctc
    10201 tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg
    10261 gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga aagtacagca
    10321 aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt tatataccac
    10381 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat
    10441 tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt
    10501 gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc
    10561 agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca
    10621 gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt
    10681 cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca
    10741 atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt
    10801 accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg
    10861 aacaaagctc ctattcccac tgctcttttt tctctctgca ccactct
//
