LOCUS       sequence-373246-       11191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered 
            PLD mutant with 10 times higher activity than the wild-type).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Tei R, Bagde SR, Fromme JC, Baskin JM
  TITLE     Activity-based directed evolution of a membrane editor in mammalian 
            cells.
  JOURNAL   Nat Chem. 2023 May 22. doi: 10.1038/s41557-023-01214-0.
  PUBMED    37217787
REFERENCE   2  (bases 1 to 11191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem. 
            2023 May 22. doi: 10.1038/s41557-023-01214-0."
COMMENT     Sequence Label: pCDH-CRY2-mCh-superPLDx10
FEATURES             Location/Qualifiers
     source          1..11191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      19..154
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(187..203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(187..203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(200..222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    211..227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(235..241,242..259,260..265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    280..301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(418..435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(589..1177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(1348..2139,2140..2208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1971..1990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2209..2313)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2381..2399
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2437..2459)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2559..2578
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2772..2794
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2786..2803
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2787..2803
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2818..3044
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3045..3225
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3272..3397
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3890..4123
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4308..4352
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4619..4735
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4825..5028
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4979..4999
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5025..5049
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5100..6593
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             6627..7331
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6769..6787)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7037..7056
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        8957..9168
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             9181..9449
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             9476..10075
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9476..9495)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9972..9992
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10082..10670
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10135..10155)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10553..10564)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10744..10977
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11049..11170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11086..11105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11140..11159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
       61 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
      121 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
      181 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      241 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      301 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      361 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      421 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      481 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      541 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      601 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      661 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      721 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      781 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      841 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      901 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      961 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1021 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1081 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1141 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1201 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1261 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1321 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1381 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1441 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1501 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1561 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1621 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1681 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1741 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1801 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1861 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1921 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1981 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2041 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2101 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2161 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2221 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2281 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2341 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     2401 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     2461 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     2521 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     2581 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     2641 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     2701 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     2761 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgacgcgtgt
     2821 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     2881 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     2941 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     3001 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     3061 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3121 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3181 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     3241 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     3301 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     3361 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     3421 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     3481 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     3541 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3601 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3661 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3721 caccgcacag caagcggcca ctgatcttca gacctggagg aggagatatg agggacaatt
     3781 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3841 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3901 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     3961 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     4021 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     4081 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     4141 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     4201 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     4261 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     4321 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     4381 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     4441 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     4501 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     4561 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     4621 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4681 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga
     4741 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     4801 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     4861 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     4921 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     4981 caaatgggcg gtaggcgtgt acggtgggag gtttatataa gcagagctcg tttagtgaac
     5041 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attgccacca
     5101 tgaagatgga caaaaagact atagtttggt ttagaagaga cctaaggatt gaggataatc
     5161 ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt tggtgtcctg
     5221 aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa caatcacttg
     5281 ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc aaaacccaca
     5341 acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa gtcgtcttta
     5401 accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag aagctggtgg
     5461 aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg tgggagatat
     5521 actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaggaaa tgcttagata
     5581 tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata actgcagcgg
     5641 ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag gccgagaaac
     5701 cgagcaatgc gttgttaact agagcttggt ctccaggatg gagcaatgct gataagttac
     5761 taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag aaagttgttg
     5821 ggaactctac ttcactactt tctccgtatc tccatttcgg ggaaataagc gtcagacacg
     5881 ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac agtgaaggag
     5941 aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct cggtatatat
     6001 gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg tttttccctt
     6061 gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt tatccgttgg
     6121 tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga ataagagtga
     6181 ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga atgaagtatt
     6241 tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg cagtatatct
     6301 ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg ttacaaggcg
     6361 ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt gcgagattgc
     6421 caactgaatg gattcatcat ccatgggacg ctcctttaac cgtactcaaa gcttctggtg
     6481 tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct cgtgagctac
     6541 tagctaaagc tatttcaaga acccgtgaag cacagatcat gatcggagca gcaggtggag
     6601 gcggttcagg cggaggtggc agcggcgtga gcaagggcga ggaggataac atggccatca
     6661 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     6721 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     6781 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     6841 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     6901 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     6961 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     7021 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     7081 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     7141 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     7201 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     7261 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     7321 agctgtacaa ggctcgaggc gcagcagcgg gagcaggagg agcaggtcga tcgggatccg
     7381 gcggatctgc agattcagct acaccacatt tggacgccgt tgagcaaaca ttgaggcaag
     7441 tgtccccagg cctggagggc gatgtatggg aaagaactag tggaaataaa ttggacggaa
     7501 gtgccgctga tcccagtgat tggttgttgc aaaccccagg atgttggggc gatgataaat
     7561 gcgctgacag agttgggaca aaaaggttgc tggctaagat gactgaaaat atagggaatg
     7621 ccaccagaac cgtagatatt agtacacttg cacctttccc aaacggcgca ttccaagacg
     7681 ctattgtcgc tggtttgaag gagtccgcag ctaaaggcaa taagttgaaa gttaggatac
     7741 tggttggggc tgcacccgtc taccacatga atgtaatacc ttccaagtat agggacgaat
     7801 tgactgcaaa acttggcaaa gccgcagaaa acataacttt gaacgtggct agtatgacta
     7861 catcaaaaac agctctctcc tggaaccata gcaaaatctt ggttgtagac gggcaaagtg
     7921 cccttactgg tggaataaat agctggaagg atgattactt ggatactact catccagtgt
     7981 ctgatgttga tcttgcactg accggaccag ctgcaggctc tgccggtagg taccttgaca
     8041 ctttgtggac atggacctgc caaaacaaga gtaacatcgc ttccgtctgg tttgcagctt
     8101 ccggcaacgc agggtgcatg cccactatgc atagagatac aaaccccaag gcatcacctg
     8161 ccaccgggaa tgtcccagta atagccgttg gagggttggg cgttggcatc aaagatgtgg
     8221 accccaagtc cactttcagg cccgatctgc caacagctag cgataccaag tgtgtggtag
     8281 gacttcacga caatactaac gccgatagag actatgatac agtcaaccca gaagagagtg
     8341 ccttgagagc tcttgttgct tcagcaaaaa gtcacattga aatatctcag caggatttga
     8401 acgctacatg tccacccttg cctaggtatg acattagatt gtacgatgcc ttggctgcta
     8461 aaatggctgc tggggttaag gtgagaattg tggttagcga cccagccaac aggggcgcag
     8521 tgggcagtgt gggttacagc caaataaaat cacttagtga aatatcagac actttgagaa
     8581 acaggcttgc aaatatcaca ggcggtcaac aagctgccaa aactgcaatg tgtagcaatt
     8641 tgcagcttgc aacctttaga tcctccccca acgacaaatg ggcagacggg catccttatg
     8701 cccaacacca taagttggtt tccgtcgact catccacatt ttatatcggt tcaaaaaatt
     8761 tgtacccatc ctggctgcaa gattttggct acatagtgga atccccagag gctgccaagc
     8821 aactgggtgc caagttgctt gaccctcagt ggaagtactc acaggagact gcaacagtgg
     8881 attacgcaag aggaatctgc aacgctgaat tctaacgcgg ccgcaaggat ctgcgatcgc
     8941 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     9001 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     9061 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     9121 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
     9181 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
     9241 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
     9301 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
     9361 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     9421 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagatgac
     9481 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     9541 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     9601 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9661 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     9721 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9781 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9841 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9901 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     9961 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    10021 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga
    10081 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
    10141 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
    10201 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
    10261 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
    10321 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
    10381 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
    10441 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
    10501 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
    10561 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
    10621 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt
    10681 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
    10741 gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt
    10801 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10861 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10921 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
    10981 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
    11041 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    11101 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    11161 atgtatctta tcatgtctgg ctctagctat c
//
