LOCUS       sequence-373302-        2218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part 1 plasmid to introduce the T4 td upstream intron and PABP 
            spacer into circRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Chen R, Wang SK, Belk JA, Amaya L, Li Z, Cardenas A, Abe BT, Chen 
            CK, Wender PA, Chang HY
  TITLE     Engineering circular RNA for enhanced protein production.
  JOURNAL   Nat Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0.
  PUBMED    35851375
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0."
COMMENT     Sequence Label: T4 td upstream intron-PABP
FEATURES             Location/Qualifiers
     source          1..2218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     176..195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(414..518)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      621..1209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1110..1129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1771..2218,1..344,345..413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
       61 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      121 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      181 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      241 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      301 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      361 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      421 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      481 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      541 cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
      601 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
      661 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      721 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      781 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      841 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      901 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      961 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1021 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1081 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1141 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1201 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1261 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc ggccgctcgg
     1321 tctcacggtg ggaattctag agaaaatttc gtctggatta gttacttatc gtgtaaaatc
     1381 tgataaatgg aattggttct acataaatgc ctaacgacta tccctttggg gagtagggtc
     1441 aagtgactcg aaacgataga caacttgctt taacaagttg gagatatagt ctgctctgca
     1501 tggtgacatg cagctggata taattccggg gtaagattaa cgaccttatc tgaacataac
     1561 gctaccgttt aatattgcgt catattcggc gaccatttgt gtggtaaaaa aaaaaaacca
     1621 aaaaaaaaaa acaaaaaaaa aaaataattg actaagatat ctgagaccgc ggccgcgatt
     1681 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1741 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1801 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1861 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gggacccacg
     1921 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1981 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2041 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2101 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2161 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgtt
//
