LOCUS       sequence-373303-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part 3-4-5 plasmid to introduce mNeonGreen into circRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Chen R, Wang SK, Belk JA, Amaya L, Li Z, Cardenas A, Abe BT, Chen 
            CK, Wender PA, Chang HY
  TITLE     Engineering circular RNA for enhanced protein production.
  JOURNAL   Nat Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0.
  PUBMED    35851375
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 18. pii: 10.1038/s41587-022-01393-0. doi: 
            10.1038/s41587-022-01393-0."
COMMENT     Sequence Label: mNeonGreen
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      38..626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     527..546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1570..2361,2362..2430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2193..2212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2431..2535)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
       61 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      721 accgtgcggc cgctcggtct caatgggatc cgtgtctaaa ggagaggaag ataacatggc
      781 gagcctccca gccactcatg aactccacat attcggaagc atcaacgggg tggactttga
      841 catggtaggt caggggaccg gcaatcccaa cgacggttac gaggaattga atctcaagtc
      901 cactaagggc gacctccagt tttctccgtg gattctcgtc ccacatatcg ggtatggttt
      961 ccaccaatac ttgccttacc cggatggaat gtcaccgttc caggctgcca tggttgatgg
     1021 ctctggttat caggtacaca gaacgatgca atttgaggac ggagcgtcat tgactgtaaa
     1081 ctacagatac acatacgaag gctctcacat aaagggcgaa gcacaagtta aaggcacggg
     1141 attccccgct gatggtcctg tcatgacaaa ttctcttacc gcagccgact ggtgtcgatc
     1201 caagaaaacg tatcccaatg acaaaactat catatctacg ttcaaatggt catatacaac
     1261 gggaaacggt aagagatacc ggagcactgc cagaaccacc tatacatttg ccaaaccaat
     1321 ggcggctaac tacctgaaga accagcccat gtatgtgttc aggaaaacgg aactgaaaca
     1381 ctccaagaca gagctgaatt tcaaggaatg gcaaaaagcc ttcacggatg ttatggggat
     1441 ggatgagctg tataaatagt tgagaccgcg gccgcgatta tcaaaaagga tcttcaccta
     1501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1681 atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc
     1741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2521 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gtcatgacca aaatccctta
     2581 acgtgagttt tcgttccact
//
