LOCUS       sequence-373304-        8518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fusion protein containing the extracellular part of 
            the human Siglec-10 A108V mutant and the Fc region of human IgG1, 
            and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Alborzian Deh Sheikh A, Gomaa S, Li X, Routledge M, Saigoh K, Numoto
            N, Angata T, Hitomi Y, Takematsu H, Tsuiji M, Ito N, Kusunoki S, 
            Tsubata T
  TITLE     A Guillain-Barre syndrome-associated SIGLEC10 rare variant impairs 
            its recognition of gangliosides.
  JOURNAL   J Autoimmun. 2021 Jan;116:102571. doi: 10.1016/j.jaut.2020.102571. 
            Epub 2020 Nov 19.
  PUBMED    33223341
REFERENCE   2  (bases 1 to 8518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Autoimmun."; date: "2021-01"; volume: "116:102571. doi"; pages: " 
            10.1016/j.jaut.2020.102571. Epub 2020 Nov 19"
COMMENT     Sequence Label: pMXs-Sig10(M2)-Fc-IG
FEATURES             Location/Qualifiers
     source          1..8518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             44..637
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    700..1057
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1116..1532
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1462..1484
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1500..1516
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1542..1916
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1854..1870
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(4568..5142,5143..5145,5146..5154)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4735..4752)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4962..4981
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5155..5157,5158..5160,5161..5874)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5155..5157,5158..5160,5161..5871)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5200..5221)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5461..5480)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5808..5829
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6045..6514
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6644..6661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6815..7403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6895..6914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7567..8358,8359..8427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8190..8209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8428..8518,1..14))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cataatgaaa gaccccacct
       61 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
      121 tgagaataga aaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
      181 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
      241 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      301 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
      361 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      421 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      481 ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
      541 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
      601 tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga
      661 gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag caacttatct
      721 gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc ggtactagtt
      781 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg gaacacccgg
      841 ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc cgacctgagt
      901 ccaaaaatcc cgatcgtttt ggactctttg gtgcaccccc ctaataggag ggatatgtgg
      961 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
     1021 tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg tgttgtctct
     1081 gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa
     1141 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     1201 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     1261 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     1321 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     1381 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     1441 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     1501 tttatccagc cctcactcct tctctaggcg cccccatatg gccatatgag atcttatatg
     1561 gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc
     1621 cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc
     1681 tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg
     1741 gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac
     1801 cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga
     1861 tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgccgg
     1921 atctagctag ttaattaagg atcccagtgt ggtggtacgg gaattcgagc tcggtacctc
     1981 gcgaatgcat ctagagccac catgctactg ccactgctgc tgtcctcgct gctgggcggg
     2041 tcccaggcta tggatgggag attctggata cgagtgcagg agtcagtgat ggtgccggag
     2101 ggcctgtgca tctctgtgcc ctgctctttc tcctaccccc gacaagactg gacagggtct
     2161 accccagctt atggctactg gttcaaagca gtgactgaga caaccaaggg tgctcctgtg
     2221 gccacaaacc accagagtcg agaggtggaa atgagcaccc ggggccgatt ccagctcact
     2281 ggggatcccg ccaaggggaa ctgctccttg gtgatcagag acgtgcagat gcaggatgag
     2341 tcacagtact tctttcgggt ggagagagga agctatgtga gatataattt catgaacgat
     2401 gggttctttc taaaagtaac agccctgact cagaagcctg atgtctacat ccccgagacc
     2461 ctggagcccg ggcagccggt gacggtcatc tgtgtgttta actgggcctt tgaggaatgt
     2521 ccaccccctt ctttctcctg gacgggggct gccctctcct cccaaggaac caaaccaacg
     2581 acctcccact tctcagtgct cagcttcacg cccagacccc aggaccacaa caccgacctc
     2641 acctgccatg tggacttctc cagaaagggt gtgagcgcac agaggaccgt ccgactccgt
     2701 gtggcctatg cccccagaga ccttgttatc agcatttcac gtgacaacac gccagccctg
     2761 gagccccagc cccagggaaa tgtcccatac ctggaagccc aaaaaggcca gttcctgcgg
     2821 ctcctctgtg ctgctgacag ccagccccct gccacactga gctgggtcct gcagaacaga
     2881 gtcctctcct cgtcccatcc ctggggccct agacccctgg ggctggagct gcccggggtg
     2941 aaggctgggg attcagggcg ctacacctgc cgagcggaga acaggcttgg ctcccagcag
     3001 cgagccctgg acctctctgt gcagtatcct ccagagaacc tgagagtgat ggtttcccaa
     3061 gcaaacagga cagtcctgga aaaccttggg aacggcacgt ctctcccagt actggagggc
     3121 caaagcctgt gcctggtctg tgtcacacac agcagccccc cagccaggct gagctggacc
     3181 cagaggggac aggttctgag cccctcccag ccctcagacc ccggggtcct ggagctgcct
     3241 cgggttcaag tggagcacga aggagagttc acctgccacg ctcggcaccc actgggctcc
     3301 cagcacgtct ctctcagcct ctccgtgcac tactccccga agctgctggg cccctcctgc
     3361 tcctgggagg ctgagggtct gcactgcagc tgctcctccc aggccagccc ggccccctct
     3421 ctgcgctggt ggcttgggga ggagctgctg gaggggaaca gcagccagga ctccttcgag
     3481 gtcaccccca gctcagccgg gccctgggcc aacagctccc tgagcctcca tggagggctc
     3541 agctccggcc tcaggctccg ctgtgaggcc tggaacgtcc atggggccca gagtggatcc
     3601 atcctgcagc tgccagataa gctcgagccc aaatcttgtg acaaaactca cacatgccca
     3661 ccgtgcccag gtaagccagc ccaggcctcg ccctccagct caaggcggga caggtgccct
     3721 agagtagcct gcatccaggg acaggcccca gccgggtgct gacacgtcca cctccatctc
     3781 ttcctcagca cctgaactcc tggggggacc gtcagtcttc ctcttccccc caaaacccaa
     3841 ggacaccctc atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca
     3901 cgaagaccct gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa
     3961 gacaaagccg cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt
     4021 cctgcaccag gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct
     4081 cccagccccc atcgagaaaa ccatctccaa agccaaaggt gggacccgtg gggtgcgagg
     4141 gccacatgga cagaggccgg ctcggcccac cctctgccct gggagtgacc gctgtaccaa
     4201 cctctgtccc tacagggcag ccccgagaac cacaggtgta caccctgccc ccatcccggg
     4261 atgagctgac caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg
     4321 acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag gccacgcctc
     4381 ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg gacaagagca
     4441 ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca tgaggctctg cacaaccact
     4501 acacgcagaa gagcctctcc ctgtccccgg gtaaatagcg gccgctacgt aaattccgcc
     4561 cccccccccc ykctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     4621 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     4681 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     4741 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     4801 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     4861 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     4921 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     4981 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5041 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5101 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg
     5161 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5221 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     5281 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     5341 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     5401 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     5461 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     5521 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5581 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     5641 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     5701 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5761 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5821 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagtcgac
     5881 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     5941 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     6001 gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
     6061 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
     6121 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     6181 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt
     6241 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     6301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     6361 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     6421 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     6481 gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt
     6541 ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac
     6601 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6661 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6721 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6781 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6841 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6901 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6961 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7021 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7081 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7141 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7201 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7261 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7321 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7381 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7441 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7501 cctagatcct tttgcggccg gccgcaaatc aatctaaagt atatatgagt aaacttggtc
     7561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7621 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7681 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7861 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8161 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8461 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaa
//
