LOCUS       sequence-373306-       19608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA for arabidopsis transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19608)
  AUTHORS   Weiss T, Crisp PA, Rai KM, Song M, Springer NM, Zhang F
  TITLE     Epigenetic features drastically impact CRISPR-Cas9 efficacy in 
            plants.
  JOURNAL   Plant Physiol. 2022 Jun 11. pii: 6605859. doi: 
            10.1093/plphys/kiac285.
  PUBMED    35689624
REFERENCE   2  (bases 1 to 19608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2022 Jun 11. pii: 6605859. doi: 10.1093/plphys/kiac285."
COMMENT     Sequence Label: pTW069
FEATURES             Location/Qualifiers
     source          1..19608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1629..2423)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2848..2872
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    2950..3124
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3131..3682)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(3727..4403)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    4594..4615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4630..4635,4636..4653,4654..4660)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4668..4684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4673..4695
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4692..4708
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4692..4708
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5238..5583
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5617..9720
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5617..9720
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9733..9753
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      9766..10014
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        10224..10569
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_RNA        10684..10759
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        10857..10932
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    11029..11203
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             12570..14222
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(12636..12654)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(14619..14636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14619..14635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14628..14650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    14838..14862
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             16163..16792
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             17221..18294
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      18360..18554
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(18790..18812)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    18898..19038
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18953..18972
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(19053..19070)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(19224..19608,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19304..19323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtgctgat gaatccccta atgattttta tcaaaatcat taagttaagg tagatacaca
      301 tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa tcagacaaca aaatgtgcga
      361 actcgatatt ttacacgact ctctttacca attctgcccc gaattacact taaaacgact
      421 caacagctta acgttggctt gccacgcctt acttgactgt aaaactctca ctcttaccga
      481 acttggccgt aacctgccaa ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg
      541 attgttaggt aatcgtcacc tccacaaaga gcgactcgct gtataccgtt ggcatgctag
      601 ctttatctgt tcgggcaata cgatgcccat tgtacttgtt gactggtctg atatccgtga
      661 gcaaaaacgg cttatggtat tgcgagcttc agtcgcacta cacggtcgtt ctgttactct
      721 ttatgagaaa gcgttcccgc tttcagagca atgttcaaag aaagctcatg accaatttct
      781 agccgacctt gcgagcattc taccgagtaa caccacaccg ctcattgtca gtgatgctgg
      841 ctttaaagtg ccatggtata aatccgttga gaagctgggt tggtactggt taagtcgagt
      901 aagaggaaaa gtacaatatg cagacctagg agcggaaaac tggaaaccta tcagcaactt
      961 acatgatatg tcatctagtc actcaaagac tttaggctat aagaggctga ctaaaagcaa
     1021 tccaatctca tgccaaattc tattgtataa atctcgctct aaaggccgaa aaaatcagcg
     1081 ctcgacacgg actcattgtc accacccgtc acctaaaatc tactcagcgt cggcaaagga
     1141 gccatggatt ctagcaacta acttacctgt tgaaattcga acacccaaac aacttgttaa
     1201 tatctattcg aagcgaatgc agattgaaga aaccttccga gacttgaaaa gtcctgccta
     1261 cggactaggc ctacgccata gccgaacgag cagctcagag cgttttgata tcatgctgct
     1321 aatcgccctg atgcttcaac taacatgttg gcttgcgggc gttcatgctc agaaacaagg
     1381 ttgggacaag cacttccagg ctaacacagt cagaaatcga aacgtactct caacagttcg
     1441 cttaggcatg gaagttttgc ggcattctgg ctacacaata acaagggaag acttactcgt
     1501 ggctgcaacc ctactagctc aaaatttatt cacacatggt tacgctttgg ggaaattatg
     1561 aggggatctc tcagcgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
     1621 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
     1681 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
     1741 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
     1801 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
     1861 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
     1921 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
     1981 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
     2041 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
     2101 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
     2161 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
     2221 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
     2281 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
     2341 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
     2401 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
     2461 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     2521 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     2581 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
     2641 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     2701 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     2761 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     2821 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     2881 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
     2941 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
     3001 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
     3061 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
     3121 gaaactcgag tcaaatctcg gtgacgggca ggaccggacg gggcggtacc ggcaggctga
     3181 agtccagctg ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg gccgcccgca
     3241 gcatgccgcg gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg tcgttgggca
     3301 gcccgatgac agcgaccacg ctcttgaagc cctgtgcctc cagggacttc agcagatggg
     3361 tgtagagcgt ggagcccagt cccgtccgct ggtggcgggg gctgacgtac acggtcgact
     3421 cggccgtcca gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg atgccggcga
     3481 cctcgccgtc cacctcggcg acgagccagg gatagcgctc ccgcagacgg acgaggtcgt
     3541 ccgtccactc ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt gtctcgatgt
     3601 agtggttgac gatggtgcag accgccggca tgtccgcctc ggtggcacgg cggatgtcgg
     3661 ccgggcgtcg ttctgggctc atggtagact cgagagagat agatttgtag agagagactg
     3721 gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     3781 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
     3841 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     3901 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
     3961 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
     4021 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     4081 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     4141 ctccaccatg ttcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     4201 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     4261 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     4321 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     4381 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     4441 tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg
     4501 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4561 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4621 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4681 acaatttcac acaggaaaca gctatgacat gattacgaat tcgagctcgg tacccgggga
     4741 tcggcgcgcc agatttgcct tttcaatttc agaaagaatg ctaacccaca gatggttaga
     4801 gaggcttacg cagcaggtat catcaagacg atctacccga gcaataatct ccaggaaatc
     4861 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag
     4921 aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
     4981 ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc
     5041 actgaatcaa aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta
     5101 aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc
     5161 gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca
     5221 gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga
     5281 ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc
     5341 tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt
     5401 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     5461 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     5521 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga
     5581 acacggggga ctcctgcagg tagatcgctc gtcgacatgg ataagaagta ctctatcgga
     5641 ctcgatatcg gaactaactc tgtgggatgg gctgtgatca ccgatgagta caaggtgcca
     5701 tctaagaagt tcaaggttct cggaaacacc gataggcact ctatcaagaa aaaccttatc
     5761 ggtgctctcc tcttcgattc tggtgaaact gctgaggcta ccagactcaa gagaaccgct
     5821 agaagaaggt acaccagaag aaagaacagg atctgctacc tccaagagat cttctctaac
     5881 gagatggcta aagtggatga ttcattcttc cacaggctcg aagagtcatt cctcgtggaa
     5941 gaagataaga agcacgagag gcaccctatc ttcggaaaca tcgttgatga ggtggcatac
     6001 cacgagaagt accctactat ctaccacctc agaaagaagc tcgttgattc tactgataag
     6061 gctgatctca ggctcatcta cctcgctctc gctcacatga tcaagttcag aggacacttc
     6121 ctcatcgagg gtgatctcaa ccctgataac tctgatgtgg ataagttgtt catccagctc
     6181 gtgcagacct acaaccagct tttcgaagag aaccctatca acgcttcagg tgtggatgct
     6241 aaggctatcc tctctgctag gctctctaag tcaagaaggc ttgagaacct cattgctcag
     6301 ctccctggtg agaagaagaa cggacttttc ggaaacttga tcgctctctc tctcggactc
     6361 acccctaact tcaagtctaa cttcgatctc gctgaggatg caaagctcca gctctcaaag
     6421 gatacctacg atgatgatct cgataacctc ctcgctcaga tcggagatca gtacgctgat
     6481 ttgttcctcg ctgctaagaa cctctctgat gctatcctcc tcagtgatat cctcagagtg
     6541 aacaccgaga tcaccaaggc tccactctca gcttctatga tcaagagata cgatgagcac
     6601 caccaggatc tcacacttct caaggctctt gttagacagc agctcccaga gaagtacaaa
     6661 gagattttct tcgatcagtc taagaacgga tacgctggtt acatcgatgg tggtgcatct
     6721 caagaagagt tctacaagtt catcaagcct atcctcgaga agatggatgg aaccgaggaa
     6781 ctcctcgtga agctcaatag agaggatctt ctcagaaagc agaggacctt cgataacgga
     6841 tctatccctc atcagatcca cctcggagag ttgcacgcta tccttagaag gcaagaggat
     6901 ttctacccat tcctcaagga taacagggaa aagattgaga agattctcac cttcagaatc
     6961 ccttactacg tgggacctct cgctagagga aactcaagat tcgcttggat gaccagaaag
     7021 tctgaggaaa ccatcacccc ttggaacttc gaagaggtgg tggataaggg tgctagtgct
     7081 cagtctttca tcgagaggat gaccaacttc gataagaacc ttccaaacga gaaggtgctc
     7141 cctaagcact ctttgctcta cgagtacttc accgtgtaca acgagttgac caaggttaag
     7201 tacgtgaccg agggaatgag gaagcctgct tttttgtcag gtgagcaaaa gaaggctatc
     7261 gttgatctct tgttcaagac caacagaaag gtgaccgtga agcagctcaa agaggattac
     7321 ttcaagaaaa tcgagtgctt cgattcagtt gagatttctg gtgttgagga taggttcaac
     7381 gcatctctcg gaacctacca cgatctcctc aagatcatta aggataagga tttcttggat
     7441 aacgaggaaa acgaggatat cttggaggat atcgttctta ccctcaccct ctttgaagat
     7501 agagagatga ttgaagaaag gctcaagacc tacgctcatc tcttcgatga taaggtgatg
     7561 aagcagttga agagaagaag atacactggt tggggaaggc tctcaagaaa gctcattaac
     7621 ggaatcaggg ataagcagtc tggaaagaca atccttgatt tcctcaagtc tgatggattc
     7681 gctaacagaa acttcatgca gctcatccac gatgattctc tcacctttaa agaggatatc
     7741 cagaaggctc aggtttcagg acagggtgat agtctccatg agcatatcgc taacctcgct
     7801 ggatctcctg caatcaagaa gggaatcctc cagactgtga aggttgtgga tgagttggtg
     7861 aaggtgatgg gaaggcataa gcctgagaac atcgtgatcg aaatggctag agagaaccag
     7921 accactcaga agggacagaa gaactctagg gaaaggatga agaggatcga ggaaggtatc
     7981 aaagagcttg gatctcagat cctcaaagag caccctgttg agaacactca gctccagaat
     8041 gagaagctct acctctacta cctccagaac ggaagggata tgtatgtgga tcaagagttg
     8101 gatatcaaca ggctctctga ttacgatgtt gatcatatcg tgccacagtc attcttgaag
     8161 gatgattcta tcgataacaa ggtgctcacc aggtctgata agaacagggg taagagtgat
     8221 aacgtgccaa gtgaagaggt tgtgaagaaa atgaagaact attggaggca gctcctcaac
     8281 gctaagctca tcactcagag aaagttcgat aacttgacta aggctgagag gggaggactc
     8341 tctgaattgg ataaggcagg attcatcaag aggcagcttg tggaaaccag gcagatcact
     8401 aagcacgttg cacagatcct cgattctagg atgaacacca agtacgatga gaacgataag
     8461 ttgatcaggg aagtgaaggt tatcaccctc aagtcaaagc tcgtgtctga tttcagaaag
     8521 gatttccaat tctacaaggt gagggaaatc aacaactacc accacgctca cgatgcttac
     8581 cttaacgctg ttgttggaac cgctctcatc aagaagtatc ctaagctcga gtcagagttc
     8641 gtgtacggtg attacaaggt gtacgatgtg aggaagatga tcgctaagtc tgagcaagag
     8701 atcggaaagg ctaccgctaa gtatttcttc tactctaaca tcatgaattt cttcaagacc
     8761 gagattaccc tcgctaacgg tgagatcaga aagaggccac tcatcgagac aaacggtgaa
     8821 acaggtgaga tcgtgtggga taagggaagg gatttcgcta ccgttagaaa ggtgctctct
     8881 atgccacagg tgaacatcgt taagaaaacc gaggtgcaga ccggtggatt ctctaaagag
     8941 tctatcctcc ctaagaggaa ctctgataag ctcattgcta ggaagaagga ttgggaccct
     9001 aagaaatacg gtggtttcga ttctcctacc gtggcttact ctgttctcgt tgtggctaag
     9061 gttgagaagg gaaagagtaa gaagctcaag tctgttaagg aacttctcgg aatcactatc
     9121 atggaaaggt catctttcga gaagaaccca atcgatttcc tcgaggctaa gggatacaaa
     9181 gaggttaaga aggatctcat catcaagctc ccaaagtact cactcttcga actcgagaac
     9241 ggtagaaaga ggatgctcgc ttctgctggt gagcttcaaa agggaaacga gcttgctctc
     9301 ccatctaagt acgttaactt tctttacctc gcttctcact acgagaagtt gaagggatct
     9361 ccagaagata acgagcagaa gcaacttttc gttgagcagc acaagcacta cttggatgag
     9421 atcatcgagc agatctctga gttctctaaa agggtgatcc tcgctgatgc aaacctcgat
     9481 aaggtgttgt ctgcttacaa caagcacaga gataagccta tcagggaaca ggcagagaac
     9541 atcatccatc tcttcaccct taccaacctc ggtgctcctg ctgctttcaa gtacttcgat
     9601 acaaccatcg ataggaagag atacacctct accaaagaag tgctcgatgc taccctcatc
     9661 catcagtcta tcactggact ctacgagact aggatcgatc tctcacagct cggtggtgat
     9721 tcaagggctg atcctaagaa gaagaggaag gtttgacgtc gacgatatga agatgaagat
     9781 gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc
     9841 ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga tcacattata
     9901 atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct cttctgcagc
     9961 atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga taagccagag
    10021 ctctggtgac ggacggcgcg catggagtca aagattcaaa tagaggacct aacagaactc
    10081 gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa
    10141 atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
    10201 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
    10261 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
    10321 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
    10381 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
    10441 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
    10501 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
    10561 gagagaacac gggggactcc tgcaggaaca aagcaccagt ggtctagtgg tagaatagta
    10621 ccctgccacg gtacagaccc gggttcgatt cccggctggt gcataggtgg actatgcagt
    10681 caagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
    10741 agtggcaccg agtcggtgca acaaagcacc agtggtctag tggtagaata gtaccctgcc
    10801 acggtacaga cccgggttcg attcccggct ggtgcacgac attcataaca gagacagttt
    10861 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
    10921 ccgagtcggt gcaacaaagc accagtggtc tagtggtaga atagtaccct gccacggtac
    10981 agacccgggt tcgattcccg gctggtgcag tcgatcgaca agctcgagtt tctccataat
    11041 aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt
    11101 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
    11161 tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa ttagagctct
    11221 accggcggcg cgccgtcgag ctgcaggtca acggatcagg atattcttgt ttaagatgtt
    11281 gaactctatg gaggtttgta tgaactgatg atctaggacc ggataagttc ccttcttcat
    11341 agcgaactta ttcaaagaat gttttgtgta tcattcttgt tacattgtta ttaatgaaaa
    11401 aatattattg gtcattggac tgaacacgag tgttaaatat ggaccaggcc ccaaataaga
    11461 tccattgata tatgaattaa ataacaagaa taaatcgagt caccaaacca cttgcctttt
    11521 ttaacgagac ttgttcacca acttgataca aaagtcatta tcctatgcaa atcaataatc
    11581 atacaaaaat atccaataac actaaaaaat taaaagaaat ggataatttc acaatatgtt
    11641 atacgataaa gaagttactt ttccaagaaa ttcactgatt ttataagccc acttgcatta
    11701 gataaatggc aaaaaaaaac aaaaaggaaa agaaataaag cacgaagaat tctagaaaat
    11761 acgaaatacg cttcaatgca gtgggaccca cggttcaatt attgccaatt ttcagctcca
    11821 ccgtatattt aaaaaataaa acgataatgc taaaaaaata taaatcgtaa cgatcgttaa
    11881 atctcaacgg ctggatctta tgacgaccgt tagaaattgt ggttgtcgac gagtcagtaa
    11941 taaacggcgt caaagtggtt gcagccggca cacacgagtc gtgtttatca actcaaagca
    12001 caaatacttt tcctcaacct aaaaataagg caattagcca aaaacaactt tgcgtgtaaa
    12061 caacgctcaa tacacgtgtc attttattat tagctattgc ttcaccgcct tagctttctc
    12121 gtgacctagt cgtcctcgtc ttttcttctt cttcttctat aaaacaatac ccaaagagct
    12181 cttcttcttc acaattcaga tttcaatttc tcaaaatctt aaaaactttc tctcaattct
    12241 ctctaccgtg atcaaggtaa atttctgtgt tccttattct ctcaaaatct tcgattttgt
    12301 tttcgttcga tcccaatttc gtatatgttc tttggtttag attctgttaa tcttagatcg
    12361 aacacgattt tctgggtttg atcgttagat atcatcttaa ttctcgatta gggtttcata
    12421 gatatcatcc gatttgttca aataatttga gttttgtcga ataattactc ttcgatttgt
    12481 gatttctatc tagatctggt gttagtttct agtttgtgcg atcgaatttg tcgattaatc
    12541 tgagtttttc tgattaacag gcctgcagga tggaagacgc caaaaacata aagaaaggcc
    12601 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
    12661 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
    12721 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
    12781 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
    12841 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
    12901 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
    12961 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
    13021 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
    13081 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
    13141 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
    13201 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa
    13261 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
    13321 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
    13381 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc
    13441 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
    13501 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt
    13561 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga
    13621 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
    13681 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt
    13741 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
    13801 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
    13861 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
    13921 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgct ggtgtcgcag
    13981 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
    14041 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
    14101 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
    14161 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt
    14221 gactcgaggt tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg
    14281 cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga
    14341 tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt
    14401 tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt
    14461 tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa
    14521 acacttgtag ttgtgagctc gcgcgtagtc ctcggtaata tcgcagaaca aaagtacctg
    14581 atatcgagtg tacttcaagt cacaccggcg agtgtggcac tggccgtcgt tttacaacgt
    14641 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    14701 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    14761 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag
    14821 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
    14881 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
    14941 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
    15001 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
    15061 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
    15121 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
    15181 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
    15241 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
    15301 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
    15361 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
    15421 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
    15481 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
    15541 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
    15601 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
    15661 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
    15721 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
    15781 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
    15841 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
    15901 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
    15961 acggctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
    16021 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
    16081 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
    16141 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
    16201 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
    16261 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
    16321 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
    16381 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
    16441 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
    16501 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
    16561 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
    16621 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
    16681 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
    16741 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
    16801 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
    16861 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
    16921 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
    16981 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
    17041 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
    17101 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
    17161 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
    17221 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
    17281 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
    17341 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
    17401 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
    17461 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
    17521 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
    17581 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
    17641 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
    17701 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
    17761 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
    17821 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
    17881 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
    17941 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
    18001 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
    18061 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
    18121 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
    18181 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
    18241 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
    18301 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg cctctttcct
    18361 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
    18421 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
    18481 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
    18541 ctggcctgtg cataactgtc tggccagcgc acagcccaag agctgcaaaa agcgcctacc
    18601 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
    18661 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
    18721 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
    18781 cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc tgtaagcgga
    18841 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
    18901 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
    18961 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    19021 agaaaatacc gcatcaggcc ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    19081 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    19141 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    19201 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    19261 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    19321 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    19381 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    19441 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    19501 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    19561 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtat
//
