LOCUS       sequence-373310-        9154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human DPYSL2 (CRMP2) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Abu Rmaileh A, Solaimuthu B, Khatib A, Lavi S, Tanna M, Hayashi A, 
            Ben Yosef M, Lichtenstein M, Pillar N, Shaul YD
  TITLE     DPYSL2 interacts with JAK1 to mediate breast cancer cell migration.
  JOURNAL   J Cell Biol. 2022 Jul 4;221(7). pii: 213220. doi: 
            10.1083/jcb.202106078. Epub 2022 May 16.
  PUBMED    35575798
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-07-4"; volume: "221(7). pii: 213220. doi"; 
            pages: " 10.1083/jcb.202106078. Epub 2022 May 16"
COMMENT     Sequence Label: DPYSL2-pLJC2
FEATURES             Location/Qualifiers
     source          1..9154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1994..2059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2107..2224
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2273..2783
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2702..2720
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2805..3404
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2805..2824)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3301..3321
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3532..3765
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3837..3958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3874..3893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3928..3947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3998..4133
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4153..4172)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4154..4172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4182..4199)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4182..4198)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4191..4213)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4340..4795
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4427..4446)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4637..4658
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4821..4925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4926..4994,4995..5786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5144..5163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5957..6545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6446..6465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6699..6716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6833..6854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6869..6874,6875..6892,6893..6899)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6907..6923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6912..6934
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6931..6947
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6931..6947
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6966..6986
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6968..6986
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7014..7240
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7241..7421
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7468..7593
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8086..8319
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8504..8548
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8846..8866
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tttaattaag agagagatgt cttatcaggg gaagaaaaat attccacgca tcacgagcga
      301 tcgtcttctg atcaaaggag gtaaaattgt taatgatgac cagtcgttct atgcagacat
      361 atacatggaa gatgggttga tcaagcaaat aggagaaaat ctgattgtgc caggaggagt
      421 gaagaccatc gaggcccact cccggatggt gatccccgga ggaattgacg tccacactcg
      481 tttccagatg cctgatcagg gaatgacgtc tgctgatgat ttcttccaag gaaccaaggc
      541 ggccctggct gggggaacca ctatgatcat tgaccacgtt gttcctgagc ctgggacaag
      601 cctgctcgct gcctttgacc agtggaggga atgggccgac agcaagtcct gctgtgacta
      661 ctctctgcat gtggacatca gcgagtggca taagggcatc caggaggaga tggaagcgct
      721 tgtgaaggat cacggggtaa attccttcct cgtgtacatg gctttcaaag atcgcttcca
      781 gctaacggat tgccagattt atgaagtact gagtgtgatc cgggatattg gcgccatagc
      841 ccaagtccac gcagaaaatg gcgacatcat tgcagaggag cagcagagga tcctggatct
      901 gggcatcacg ggccccgagg gacatgtgct gagccgacct gaggaggtcg aggccgaagc
      961 cgtgaatcgt gccatcacca tcgccaacca gaccaactgc ccgctgtata tcaccaaggt
     1021 gatgagcaaa agctctgctg aggtcatcgc ccaggcacgg aagaagggaa ctgtggtgta
     1081 tggcgagccc atcactgcca gcttgggaac ggacggctcc cattactgga gcaagaactg
     1141 ggccaaggct gctgcctttg tcacctcccc acccttgagc cctgatccaa ccactccaga
     1201 ctttctcaac tccttgctgt cctgtggaga cctccaggtc acgggcagtg cccattgcac
     1261 gtttaacact gcccagaagg ctgtaggaaa ggacaacttc accctgattc cggagggcac
     1321 caatggcact gaggagcgga tgtccgtcat ctgggacaag gctgtggtca ctgggaagat
     1381 ggatgagaac cagtttgtgg ctgtgaccag caccaatgca gccaaagtct tcaaccttta
     1441 cccccggaaa ggccgcattg ctgtgggatc cgatgccgac ctggtcatct gggaccccga
     1501 cagcgttaaa accatctctg ccaagacaca caacagctct ctcgagtaca acatctttga
     1561 aggcatggag tgccgcggct ccccactggt ggtcatcagc caggggaaga ttgtcctgga
     1621 ggacggcacc ctgcatgtca ccgaaggctc tggacgctac attccccgga agcccttccc
     1681 tgattttgtt tacaagcgta tcaaggcaag gagcaggctg gctgagctga gaggggttcc
     1741 tcgtggcctg tatgacggac ctgtgtgtga agtgtctgtg acgcccaaga cagtcactcc
     1801 agcctcctcg gccaagacgt ctcctgccaa gcagcaggcc ccacctgtcc ggaacctgca
     1861 ccagtctgga ttcagtttgt ctggtgctca gattgatgac aacattcccc gccgcaccac
     1921 ccagcgtatc gtggcgcccc ccggtggccg tgccaacatc accagcctgg gcggaggcag
     1981 cgcggccgca ggcgactaca aagaccatga cggtgattat aaagatcatg acattgatta
     2041 caaggatgac gatgacaagt aataagaatt ctcgacctcg agacaaatgg cagtattcat
     2101 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2161 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2221 ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg
     2281 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     2341 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     2401 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     2461 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     2521 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     2581 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     2641 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     2701 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     2761 gaatcaccga cctctctccc cagggggatc caccggagct taccatgacc gagtacaagc
     2821 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     2881 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     2941 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     3001 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     3061 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     3121 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     3181 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     3241 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     3301 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     3361 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     3421 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta agaccaatga
     3481 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3541 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3601 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3661 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3721 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     3781 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     3841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3961 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4021 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4081 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     4141 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4201 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4261 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4321 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4381 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4441 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4501 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4561 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4621 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4681 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4741 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     4801 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4861 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4921 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4981 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5041 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5101 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5161 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5221 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5281 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5341 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5401 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5461 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5521 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5581 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5641 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5701 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5761 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5821 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5881 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5941 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6001 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6061 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6361 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6421 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6481 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6541 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6601 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6661 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6721 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6781 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     6841 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     6901 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     6961 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     7021 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     7081 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     7141 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     7201 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     7261 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7321 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7381 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     7441 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     7501 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     7561 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     7621 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     7681 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     7741 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     7801 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     7861 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     7921 gcacagcaag cagcagctga tcttcagacc tggaggagga gatatgaggg acaattggag
     7981 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8041 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8101 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8161 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8221 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8281 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8341 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8401 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     8461 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     8521 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     8581 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     8641 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     8701 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     8761 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat cacgagacta
     8821 gcctcgagca gcagccccct tcaccgaggg cctatttccc atgattcctt catatttgca
     8881 tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
     8941 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
     9001 attatgtttt aaaatggact atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     9061 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     9121 gtacatctac gtattagtca tcgctattac catg
//
