LOCUS       sequence-373317-        9784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GPa3b17 hTCR alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Susac L, Vuong MT, Thomas C, von Bulow S, O'Brien-Ball C, Santos AM,
            Fernandes RA, Hummer G, Tampe R, Davis SJ
  TITLE     Structure of a fully assembled tumor-specific T cell receptor 
            ligated by pMHC.
  JOURNAL   Cell. 2022 Aug 18;185(17):3201-3213.e19. doi: 
            10.1016/j.cell.2022.07.010.
  PUBMED    35985289
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.07"; journalName: "Cell"; date: "2022-08-18- 
            18"; volume: "185"; issue: "17"; pages: "3201-3213.e19"
COMMENT     Sequence Label: pHRSin_hTCR-alpha
FEATURES             Location/Qualifiers
     source          1..9784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             94..327
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(352..370)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(353..370)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(660..679)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(893..912)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1012..1034
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1072..1090)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1158..1262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1263..1331,1332..2123)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1481..1500)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2294..2882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2783..2802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3036..3053
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3123..3143)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3128..3457
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3308..3443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4591..4656
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4786..4806
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5231..5365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5268..5287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5322..5341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5533..6166
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6213..6338
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6835..7068
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7253..7297
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7582..7699
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7852..8259
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    9180..9768
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9233..9253)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9651..9662)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(9771..9784,1..3))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(9772..9784,1..4))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
       61 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
      121 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
      181 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      241 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      301 tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg
      361 tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa
      421 cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc
      481 tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa
      541 cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat
      601 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
      661 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
      721 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
      781 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
      841 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct
      901 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      961 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     1021 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     1081 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1141 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1201 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1261 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1321 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1381 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1441 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1501 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1561 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1621 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1681 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1741 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1801 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1861 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1921 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1981 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2041 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2101 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2161 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2221 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2281 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2341 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2401 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2461 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2521 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2581 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2641 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2701 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2761 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2821 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2881 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2941 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3001 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3061 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     3121 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3361 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3421 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     3481 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     3541 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     3601 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3661 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3721 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     3781 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     3841 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     3901 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     3961 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     4021 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     4081 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     4141 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     4201 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     4261 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
     4321 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     4381 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
     4441 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
     4501 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     4561 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     4621 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     4681 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     4741 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     4801 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     4861 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     4921 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     4981 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     5041 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     5101 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     5161 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     5221 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5341 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     5401 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     5461 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     5521 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     5581 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     5641 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     5701 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     5761 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     5821 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     5881 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     5941 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     6001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     6181 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     6241 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     6301 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     6361 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     6421 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     6481 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     6541 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     6601 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     6661 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     6721 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     6781 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     6841 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     6901 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     6961 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7021 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7081 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7141 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     7201 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     7261 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     7321 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     7381 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     7441 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     7501 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca
     7561 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     7621 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     7681 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggatcga
     7741 taagcttgat atcgaattcc tgcagccccg ataaaataaa agattttatt tagtctccag
     7801 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctgc agtaacgcca
     7861 ttttgcaagg catggaaaaa taccaaacca agaatagaga agttcagatc aagggcgggt
     7921 acatgaaaat agctaacgtt gggccaaaca ggatatctgc ggtgagcagt ttcggccccg
     7981 gcccggggcc aagaacagat ggtcaccgca gtttcggccc cggcccgagg ccaagaacag
     8041 atggtcccca gatatggccc aaccctcagc agtttcttaa gacccatcag atgtttccag
     8101 gctcccccaa ggacctgaaa tgaccctgcg ccttatttga attaaccaat cagcctgctt
     8161 ctcgcttctg ttcgcgcgct tctgcttccc gagctctata aaagagctca caacccctca
     8221 ctcggcgcgc cagtcctccg acagactgag tcgcccgggg gggatctgga gctctcgaga
     8281 attctcacgc gtgccaccat gctcctgctg ctcgtcccag tgctcgaggt gatttttacc
     8341 ctgggaggaa ccagagccat gagtcaacaa ggagaagaag atccgcaggc cttgagcatc
     8401 caggagggtg aaaatgccac catgaactgc agttacaaaa ctagtataaa caatttacag
     8461 tggtatagac aaaattcagg tagaggcctt gtccacctaa ttttaatacg ttcaaatgaa
     8521 agagagaaac acagtggaag attaagagtc acgcttgaca cttccaagaa aagcagttcc
     8581 ttgttgatca cggcttcccg ggcagcagac actgcttctt acttctgtgc tacggacgga
     8641 tcgactccga tgcagtttgg aaagggcaca agactttctg tgattccaaa tatccagaac
     8701 cctgaccctg ccgtgtacca gctgagagac tctaaatcca gtgacaagtc tgtctgccta
     8761 ttcaccgatt ttgattctca aacaaatgtg tcacaaagta aggattctga tgtgtatatc
     8821 acagacaaaa ctgtgctaga catgaggtct atggacttca agagcaacag tgctgtggcc
     8881 tggagcaaca aatctgactt tgcatgtgca aacgccttca acaacagcat tattccagaa
     8941 gacaccttct tccccagccc agaaagttcc tgtgatgtca agctggtcga gaaaagcttt
     9001 gaaacagata cgaacctaaa ctttcaaaac ctgtcagtga ttgggttccg aatcctcctc
     9061 ctgaaagtgg ccgggtttaa tctgctcatg acgctgcggc tgtggtccag cggatcctaa
     9121 gcggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata
     9181 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     9241 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     9301 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     9361 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     9421 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     9481 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     9541 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     9601 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     9661 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     9721 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     9781 gacc
//
