LOCUS       sequence-373330-       10171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human Vimentin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Abu Rmaileh A, Solaimuthu B, Khatib A, Lavi S, Tanna M, Hayashi A, 
            Ben Yosef M, Lichtenstein M, Pillar N, Shaul YD
  TITLE     DPYSL2 interacts with JAK1 to mediate breast cancer cell migration.
  JOURNAL   J Cell Biol. 2022 Jul 4;221(7). pii: 213220. doi: 
            10.1083/jcb.202106078. Epub 2022 May 16.
  PUBMED    35575798
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-07-4"; volume: "221(7). pii: 213220. doi"; 
            pages: " 10.1083/jcb.202106078. Epub 2022 May 16"
COMMENT     Sequence Label: Vimentin-pLJM5
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1815..1824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1821..2531
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1966..1984)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2234..2253
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2583..2700
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3192..3211
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3277..4302
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3277..4302
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             4679..4912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4984..5105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5021..5040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5075..5094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5145..5280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5300..5319)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5301..5319)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5329..5346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5329..5345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5338..5360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5487..5942
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5574..5593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5784..5805
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5968..6072
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6073..6141,6142..6933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6291..6310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7104..7692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7593..7612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7846..7863
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7980..8001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8016..8021,8022..8039,8040..8046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8054..8070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8059..8081
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8078..8094
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8078..8094
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8113..8133
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8115..8133
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8161..8387
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8388..8568
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8615..8740
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9233..9466
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9651..9695
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     9993..10013
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgccaccat gtccaccagg tccgtgtcct cgtcctccta ccgcaggatg ttcggcggcc
      421 cgggcaccgc gagccggccg agctccagcc ggagctacgt gactacgtcc acccgcacct
      481 acagcctggg cagcgcgctg cgccccagca ccagccgcag cctctacgcc tcgtccccgg
      541 gcggcgtgta tgccacgcgc tcctctgccg tgcgcctgcg gagcagcgtg cccggggtgc
      601 ggctcctgca ggactcggtg gacttctcgc tggccgacgc catcaacacc gagttcaaga
      661 acacccgcac caacgagaag gtggagctgc aggagctgaa tgaccgcttc gccaactaca
      721 tcgacaaggt gcgcttcctg gagcagcaga ataagatcct gctggccgag ctcgagcagc
      781 tcaagggcca aggcaagtcg cgcctggggg acctctacga ggaggagatg cgggagctgc
      841 gccggcaggt ggaccagcta accaacgaca aagcccgcgt cgaggtggag cgcgacaacc
      901 tggccgagga catcatgcgc ctccgggaga aattgcagga ggagatgctt cagagagagg
      961 aagccgaaaa caccctgcaa tctttcagac aggatgttga caatgcgtct ctggcacgtc
     1021 ttgaccttga acgcaaagtg gaatctttgc aagaagagat tgcctttttg aagaaactcc
     1081 acgaagagga aatccaggag ctgcaggctc agattcagga acagcatgtc caaatcgatg
     1141 tggatgtttc caagcctgac ctcacggctg ccctgcgtga cgtacgtcag caatatgaaa
     1201 gtgtggctgc caagaacctg caggaggcag aagaatggta caaatccaag tttgctgacc
     1261 tctctgaggc tgccaaccgg aacaatgacg ccctgcgcca ggcaaagcag gagtccactg
     1321 agtaccggag acaggtgcag tccctcacct gtgaagtgga tgcccttaaa ggaaccaatg
     1381 agtccctgga acgccagatg cgtgaaatgg aagagaactt tgccgttgaa gctgctaact
     1441 accaagacac tattggccgc ctgcaggatg agattcagaa tatgaaggag gaaatggctc
     1501 gtcaccttcg tgaataccaa gacctgctca atgttaagat ggcccttgac attgagattg
     1561 ccacctacag gaagctgctg gaaggcgagg agagcaggat ttctctgcct cttccaaact
     1621 tttcctccct gaacctgagg gaaactaatc tggattcact ccctctggtt gatacccact
     1681 caaaaaggac acttctgatt aagacggttg aaactagaga tggacaggtt atcaacgaaa
     1741 cttctcagca tcacgatgac cttgaaagca gcggtggagc aagcgcagcc agtggtagcg
     1801 cggatccacc ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca
     1861 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     1921 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     1981 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     2041 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     2101 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     2161 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     2221 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     2281 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     2341 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     2401 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     2461 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     2521 tgtacaagta accggtacca tgaattctcg acctcgagac aaatggcagt attcatccac
     2581 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2641 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2701 cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg ggttggggtt
     2761 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     2821 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     2881 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     2941 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     3001 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     3061 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     3121 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     3181 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     3241 ctcatctccg ggcctttcga cctgcatccc gccaccatga aaaagcctga actcaccgcg
     3301 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     3361 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     3421 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     3481 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     3541 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     3601 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     3661 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     3721 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     3781 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     3841 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     3901 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     3961 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     4021 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     4081 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     4141 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     4201 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     4261 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac
     4321 aagagctgat ttcgagaacg cctcagccag caactcgcgc gagcctagca aggcaaatgc
     4381 gagagaacgg ccttacgctt ggtggcacag ttctcgtcca cagttcgcta agctcgctcg
     4441 gctgggtcgc gggagggccg gtcgcagtga ttcaggccct tctggattgt gttggtcccc
     4501 agggcacgat tgtcatgccc acgcactcgg gtgatctgac tgatcccgca gattggagat
     4561 cgccgcccgt gcctgccgat tgggtgcaga tccgtcgacc tgcagccaag ctctttaaga
     4621 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4681 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4741 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4801 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4861 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     4921 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     4981 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5041 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5101 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     5161 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5221 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5281 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     5341 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     5401 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     5461 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     5521 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5581 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5641 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5701 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5761 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5821 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5881 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     5941 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6001 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6061 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6121 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6181 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6241 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6301 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6361 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6421 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6481 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6541 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6601 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6661 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6721 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6781 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6841 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6901 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6961 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     7261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7741 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7801 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7861 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7921 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7981 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     8041 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     8101 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     8161 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     8221 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     8281 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     8341 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     8401 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8461 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8521 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     8581 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     8641 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     8701 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     8761 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     8821 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     8881 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     8941 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     9001 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     9061 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9121 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9181 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     9241 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     9301 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     9361 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     9421 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     9481 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     9541 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     9601 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     9661 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     9721 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     9781 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     9841 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     9901 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     9961 gagactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat
    10021 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
    10081 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
    10141 ttttaaaatt atgttttaaa atggactatc a
//
