LOCUS       sequence-373332-       12595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puromycin-selectable expression of HA-tagged Dora (CG34401) in 
            Drosophila S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Kingston ER, Blodgett LW, Bartel DP
  TITLE     Endogenous transcripts direct microRNA degradation in Drosophila, 
            and this targeted degradation is required for proper embryonic 
            development.
  JOURNAL   Mol Cell. 2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 
            10.1016/j.molcel.2022.08.029.
  PUBMED    36150386
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 
            10.1016/j.molcel.2022.08.029."
COMMENT     Sequence Label: HA-doraWT_rescue_construct
FEATURES             Location/Qualifiers
     source          1..12595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1646..1672
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1685..1711
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1724..1750
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8162..8215
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8240..8839
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8240..8259)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8736..8756
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8847..8981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8871..8890)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8925..8944
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9582..10170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9662..9681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10341..11132,11133..11201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10964..10983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11202..11306)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11374..11392
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_RNA        complement(11544..11619)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tggcggggtc tgtggctggg cgcgacgtca ccgacgttgg agccactcct ttgaccatgt
       61 gtgcgtgtgt gtattattcg tgtctcgcca ctcgccggtt gtttttttct ttttatgctg
      121 cgctctctct agcgccatct cgcttacgca tgctcaacgc accgcatgtt gccgtttcct
      181 tttatgcgtc attttggctc gaaataggca attatttaaa caaagattag tcaacgaaaa
      241 cgctaaaata aataagtcta caatatggtt acttattgcc atgtgtgtgc agccaacgat
      301 agcaacaaaa gcaacaacac aggtggcttt ccctctttca ctttttgttt gcaagccgcg
      361 tgcgagcaag acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt
      421 tggcgaaaaa catcaaggcg cctgatacga atgcatttgc aataacaatt gcgatattta
      481 atattgttta tgaagctgtt tgacttcaaa acacacaaaa aaaaaaataa aacaaattat
      541 ttgaaagaga attaggaatc ggacgcttat cgttagggta acaacaagaa atgcttactg
      601 agtcacagcc tctggaaaac tgccgcaagc cagagagaga gagaaaaaga gggagagcag
      661 cttagaccgc atgtgcttgt gtgtgaggcg tctctctctt cgtctctgtt gcgcaaacgc
      721 atagactgca ctgaaaaaat cgattaccta ttttttatga atgaatattt gcactattac
      781 tattcaaaac tattaagata gcaatcacat tcaatagcca aatactatac cacctgagcg
      841 atgcaacgaa atgatcaatt tgagcaaaaa tgctgcatat ttaggacggc atcattatag
      901 aaatgcttct tgctgtgtac ttttctctcg tctggcagct gtttcgccgt tattgttaaa
      961 accggcttaa gttaggtgtg ttttctacga ctagtgaatg ccctactaga agatgtgtgt
     1021 tgcacaaaat gtccctggaa taaccaattt gaagtgcaga tagcagtaaa cgtaagctaa
     1081 tatgaatatt atttaactgt aatgttttaa tatcgctgga cattactaat aaacccacta
     1141 taaacacatg tacatatgta tgttttggca tacaatgagt agttggggaa aaaatgtgta
     1201 aaagcaccgt gaccatcaca gcataaagat aaccagctga agtatcgaat atgagtaacc
     1261 cccaaattga atcacatgcc gcaactgata ggacccatgg aagtacactc ttcatggcga
     1321 tatacaagac acacacaagc acgaacaccc agttgcggag gaaattctcc gtaaatgaaa
     1381 acccaatcgg cgaacaattc atacccatat atggtaaaag ttttgaacgc gacttgagag
     1441 cggagagcat tgcggctgat aaggttttag cgctaagcgg gctttataaa acgggctgcg
     1501 ggaccagttt tcatatcact accgtttgag ttcttgtgct gtgtggatac tcctcccgac
     1561 acaaagccgc tccatcagcc agcagtcgtc taatccagag accccggatc ggggtaccta
     1621 ctagtccagt gtggtggaat tcatgtatcc atatgatgtt ccagattatg ctggaggttc
     1681 ttcatacccc tatgatgtgc ctgactacgc tggcagcgcc ggataccctt atgatgtgcc
     1741 tgattatgct ggagggagcg gcggaagtgg gagttcagga ggctccggat caagcggcag
     1801 tagtggtgga agcgaccgct tcagcttcga cgactccatc cgcttcgagg aagactccct
     1861 ctgcagctgg agctctgaac cggaatcgct gtgcaacaac tggcgcggct ggaagaagcc
     1921 cgctgcaggc agcggcgcag gcaatggcgg cttgagcggc atgggcggta tggctgtggc
     1981 cggcggctca ggagcaccgc cgttcggagg agcaggagcc tctggttcct gcacaccggg
     2041 cggcaatgga acggccatgg ccaacacatc cacctgcggc agacattatg aagtatccac
     2101 attggccgaa ctggcagccc gctgtgtggc ctcctacata ccgttcgagc tggttgagca
     2161 cgtgtatccg ccggtgccgg agcagctgca gctgcgcatt gcgttctgga gctttccgga
     2221 caacgaggag gacatccggc tctactcctg tctggccaat agctcggcgg acgagttcaa
     2281 tcgcggcgac tcgttgtttc ggttgcgagc tgtcaaggat ccgctgcaga taggctttca
     2341 cctctcggcc agcgttgtca atcagacgcc gcgtgcctat ttcaatgtgg ccgtcacctt
     2401 tgaccgccga cgcatctcct cttgcaactg cacctgcacc tcgtccgcct actggtgctc
     2461 ccacgtagtc gccgtttgtc tgcatcgcat ccattgcccc caggaggtct gtctgcgtgc
     2521 cccggtatcg gaatcattga ctcgcctgca acgcgaccaa ttgcaaaagt ttgcccagta
     2581 tctgatcagc gagctgcctc agcagatctt gccgacggct cagcggctcc tggatgagtt
     2641 gctcagtgcg caacccacgg cgatcaacac tgtgtgcggt gcacctgatc ccacggctgg
     2701 ggcctcgatc aacgaccaga ccagctggta tctggacgag aagacgttgc ataacaacat
     2761 taaacgaatc ctcatcaagt tctgtttgcc cgccccgatt gtgtttagcg acgtaaacta
     2821 tctgacgaac tcggctcccc cagcggcggc cgaatggagc tcgctactcc gaccgctgcg
     2881 gggacgtgag ccggagggca tgtggaatct actttcgatc gtacgcgaga tgtacaggcg
     2941 ctgtgatcgg aacgcagtgc gtctgctgga gatcatcacg gaggagtgct tgtactgcga
     3001 tcagatactc atctggtggt tccagacgaa gttggcactg atgatggggt cccacggcca
     3061 ctccggcggt aagcattcta acactcactc caactccacg gcactgcagc atgcttgcag
     3121 ttcgctgtgc gacgaaatcg tggcactgtg gcgattggca gcgctcaatc ccggcctggc
     3181 gccggatgag cgagatatgc tgcacgctca gttcacggcc tggcatctaa agattcttga
     3241 ccgggtggtc aagagccgaa tgatgccgtc gtacaccaac aagcaccagc agaactcgcg
     3301 atccgagacg gagctgttta tcggcttcaa gccggccatc gaggcgtgct acttggactg
     3361 ggagggctac cccataccgg gagtgacgca cacccacgac acgaatccca tctactactc
     3421 gccgttcact tgcttcaagc acacggacct caagggcgag tctaacaatc ccggccagct
     3481 gaatgccacc caagcgctga tgtcgaacaa caagcactac aattacttta gttcatccag
     3541 cgatcatggc atgcacgcag gagccttcaa gcagaggttg gaccggcctt ttcgtgagtc
     3601 gcgattctat acgaatccgg ccgatctgga cggaggagca gcgttggcgg gcggaggatc
     3661 cggtaggttt tccacgggac tgggctccgt tgccgttgga ggagctggtg caggaggctt
     3721 gggtcagatg agcggaggag ttaacaactc tgttggagga tcagcggccg ccgaagtgaa
     3781 tcccgtgcag cagcagcttc ccagtggctc ggcgggagtc ggtgcctctg tgacaaatgc
     3841 caaggttgtg gtagcaacag atggcaatcg ttcgagtgcc agcagcgagg gattctgcga
     3901 gaacgacgat ttcggcggcg acaccagcag cagtcacaac tactgtccac ctggacaggc
     3961 ggttgtgcct ggccagaaat cagccctcac ggagtcggat tcgcagagca gcttcgatgc
     4021 agtgtcgcat cagagcaagg atgagccacc agtcggggga gtgggtgtgg cagtgggtgt
     4081 tgaacaagcc ctttccacat ctgacacttc gtcggcctcc tcctcatcgt catctgcttc
     4141 tacagcttca gggagctcta atagttccac ttcctctatg cttatggctg gacaggaggc
     4201 tactgtcggt gttggtggcg ctgtgcagca aacaactcgt cgcctcagca aggatgaatc
     4261 ctttagtagc agtagcgacg agttcaacca gggaggcgtg ggtggatcag caggtcgagt
     4321 ggctatggcc tccagtggaa gcggatcggg tgctggcgac acagtagcgg tgggatcggg
     4381 atcaacagct ggcggagatc tgacgccggt gcccagcacg tcggcagctg cccgtgctgc
     4441 cttggccgcc agctcgacag ggccggtgat ttctgcttca cctcatctcg cagctagcgc
     4501 cgctggaggc ttaggagtgc tgggtgtggg tgcagaccag ccttgcacct catccgcgca
     4561 tgcagcccga gcactggcag ctgcagtggc tgcttccagc gataagccgc atgttttctc
     4621 caatgtacgg cccacagagg acgcctggga tatactgctg gccagagccg agggtttgca
     4681 tgcccacggt cacggagccg aggcttgcat cctggccgtc cgtctggccg aacagatgct
     4741 ggcgaatccg cctaacctac ttctggaact gccacccgca cccaaaagaa agggcaagaa
     4801 gcaaaacgtg aatcccatct cgcatcagct gacagttgtg gcctctgcaa cgctgtccaa
     4861 gtgcgctttt ctgtgcacag tgctgtcgga gaactctgaa cactatcaca ttggctttcg
     4921 gatatgccta ttcgccttgg agatgccgcg accgccagca agcaccaagc ccctggaagt
     4981 caagctggcc aaccaggagg cggacatact ggctctacta aagcgcctgc cgctgggatc
     5041 cgcggaactg caagtaatcc gagagcgagc tgagcaactg cgcagcggaa cctttaagac
     5101 gcgaggagag gctctattgc ctattaacct ggccacgttt attttcgatg ccctagttac
     5161 actcagcccc ttaggtggat ccacaattgt ccctggcgtg gcatccacta gtggtgtatc
     5221 aagtgcggcc ggcaagtcta tggtcaatac aggcacacgt ttgctgctct acaagcacaa
     5281 cagcgatgag agtctgggat tcgatgctgc agtggctgct ctcggactaa aagccaatgt
     5341 gtcggaggca gagcatccgt tgttgtgcga aggaacccgc cggcagcgcg gggatcttgc
     5401 tctgacattg ctttcacact acaaggatga gccccgcaag atagccaaga tcatggagaa
     5461 gcttctggac agggacatac atacgcttct aaatgcgcca ctcctgcccg cttactactc
     5521 gagtaacccg cctgtgagga cgcgaagtaa tcagcccacg cgtagggagg atcacgatta
     5581 tggcggtggc tctggatgcg cctcttccaa ctgcaatccg gtagccaatt tgtgtgaact
     5641 tttgccggcg gactacggca gtgtgggtgg aaacagtcga ccacacagct ccacgagtgc
     5701 cgaactggaa ctaagcatgt gtgccctgag catggccagc agtggtagcc agtcgggagg
     5761 cgttcaaggc atggtgccca caacgaatgc agcgggaacc acgggaacac ccagttccag
     5821 cagcacgacg gtcagtggga gccagaatcc caatggtaat cccagtggca gcggtggagg
     5881 aggaaatggc ggaggtggta acggaggagg aggaggcggt ggcggtggtg gtggaggctc
     5941 caccagtagc cgaagcaagg aaagccggta caagggaaag agagcatatc cctcgatacc
     6001 caaccagcca tcggaagcca gtgctcactt tatgttcgag ctggccaaga atgtgctgac
     6061 caaggccggc ggcaatagtt ccacatcgct cttcacccag gcgagcacga gccaaaatca
     6121 ccatggaccg caccgtgccc tccacatgtg cgccttccaa ctagggctgt atgccctcgg
     6181 tctgcacaac tgtgtgagtc ccaattggct atcgaggacg tactcgtcgc acgtctcctg
     6241 gatcctgggg caagctatgg aaattggtgc gccggcgata agctttctga tagacacttg
     6301 ggaggcgcat ctaacgcctc ccgaagcagc tggcatggcc gatcgtgcgt cgcgcggatg
     6361 ggacagcaac atggtctatc cggcggcgga gctggctctg tccgttctgc cccacgcagc
     6421 tgccctcaat cccaacgaga tccagcgtgc gattctgcag tgcaaggagc aaagcgatct
     6481 gatgctggag cgggcctgtc tcaccgtgga gacagctgcc aagggcggcg gcgtctatcc
     6541 ggaggtgcta ttccaggtgg cccgctactg gtacgagctg tacatgcgga acacgccgaa
     6601 taacagcgag ttcgagccgc acgatgacac catggaccat tcggccgtga gtctgagcgc
     6661 cctcattgag tcccagcagc agcatgagct ccagcaacaa caacaggcag tgcagcagca
     6721 acaggcggtt cagcagcagc agcaggtggt gcagcaacag cagcaagtgg tgcagcagca
     6781 acaacagctt ggcatgccca attccgtggt gccaggcggc gttggccccg gccccggact
     6841 acccgtagca gtgcagccac ccgtagttgg ttctgtccag aatccacagg cccaattcca
     6901 acccgtgggc gtcgcctcac tggctcccct cggactggcg cagtatccac cgtacagctt
     6961 ctgccagggc ttgtatgcgc accaccacaa catgagctat ccgcccgggc agatgcagat
     7021 gttcatttcg gcagggccgc cgccgccacc tcaagcatac ggcggctacc agcagccgcc
     7081 gccacagcag ccgccgaatc cgcagcaaca gcagcaggtt gtgcaacagc aggtgcagca
     7141 gcagcaggtg gtgcaacagc aacagcagca ggttcagatg gccgggcagg cgccaccagg
     7201 tcaccctggg cagcaaggac atccaggcca accgccctct ggcttccagc cgcaaccacc
     7261 gccaggagca ttccaggcgc tgccgccgca ggcctatcag gcaatgcaag cgggtccccc
     7321 gggcccgccg atgggtccgc ctcagggcta ctatggtcca ccaccaccac cgcctccgaa
     7381 cggtccgcct ggcgtgggcg tgggcgtcgg agtcggcgtt ggagtgggcg tgatgccaat
     7441 gcgccagcat cagcaccaac atcccgttta cccattcatg cagcaggcgc cgccgcagcc
     7501 accgcagcag caacaaccgc cgccctcgca gccacccgtg cgacaacgac aaccacatca
     7561 gtttacaccc acccagctgc gatatctgct ggctgcctac aatgtgggca tgctggccat
     7621 ggaaacactg gcgcgacgcg tccacgacga tcggccgcag gcgaagtatg cccgcaatcc
     7681 gccgtacggc gaggatgtga aatggttgct acggatcagc aagaaactgg gcacccagta
     7741 tctgcaccag ttctgcatct gtgcggtcaa ctcgatcgtg agtccgttcg tgctgcatga
     7801 cgtggccatt gagtccgccc actatctggg caggaacaac caccagatgg tgatgcagca
     7861 cctgcgttct gcgctcacgc ctctcgtcca gaagtgtcag cagatgtaca tccaatgcat
     7921 ccatcagaag ctgtatcatc tgacccaggg cgactatgag gagtttgcca gcattgtagt
     7981 ggcggcacgc gccgctttcc agatcacgcc ggaggggaac gcgcaattca aggactggct
     8041 gcagtctatc aagcgatcga aatcatgcaa aaaggaactg tggacccaga tcaatgcggc
     8101 cctgcagagc aactccaaaa aggccggcca ggcaaaaaag aaaaagggat caggcaagct
     8161 tgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctgctag
     8221 cggtagcggc agcggtagca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8281 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8341 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8401 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     8461 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     8521 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     8581 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     8641 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     8701 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     8761 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8821 ccgcaagccc ggtgcctgac tcgagggatc cagacatgat aagatacatt gatgagtttg
     8881 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     8941 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     9001 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     9061 acaaatgtgg tatggctgat tatgatcagt cgaccgatgc ccttgagagc cttcaaccca
     9121 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     9181 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     9241 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     9301 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     9361 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     9421 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     9481 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggccag
     9541 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9601 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9661 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9721 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9781 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9841 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9901 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9961 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10021 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10081 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10141 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10201 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10261 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10321 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10381 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10441 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10501 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10561 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10621 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
    10681 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10741 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10801 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10861 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10921 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
    10981 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11041 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11101 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11161 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11221 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11281 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    11341 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    11401 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
    11461 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
    11521 atcatttttc cagatctaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
    11581 actagcctta ttttaacttg ctatttctag ctctaaaact acataacgaa taaaaaagga
    11641 tttaagtgca aatggtaaaa aagtgtcgag tttttcttga gttgattgtg ctgtaatgag
    11701 actctgcatt cggcatttga ctcggctttt cctactcgtg ccgtatttca ggctgcaagt
    11761 cgaacagatc tcgctgcctg ttatgtggcc cacaaaccaa gacacgtttt atggccatta
    11821 aagctggctg atcgtcgcca aacaccaaat acataatgaa tatgtacaca ttcgagaaag
    11881 aagcgatcaa agaagcgtct tcgggcggag taggagaatg cggaggagaa ggagaacgag
    11941 ctgatctagt atctctccac aatccaatgc caactgacca actggccata ttcggagcaa
    12001 tttgaagcca atttccatcg cctggcgatc gctccattct tggctatatg tttttcaccg
    12061 ttacccgggg ccattttcaa agactcgtcg gcaagataag attgtgtcac tcgctgtctc
    12121 tcttcatttg tcgaagaatg ctgaggaatt tcgcgatgac gtcggcgagt attttgaaga
    12181 atgagaataa tttgtattta tacgaaaatc agttagtgga attttctaca aaaacatgtt
    12241 atctatagat aattttgttg caaaatatgt tgactatgac aaagattgta tgtatatctt
    12301 taatgtattc tcattttctt atgtatttat aatggcaatg atgatactga tgatatttta
    12361 agatgatgcc agaccaaaag gcttgaattt ctgcgtcttt tgccgaacgc agtgcatgtg
    12421 caattgttgt tttttggaat attcaatttt cggactgtcc gctttgattt cagtttcttg
    12481 gcttattcaa aaagcaaagt aaagccaaaa aagcgagatg gcaataccaa atgcggcaaa
    12541 acggtagtgg aaggaaaggg gtgcggggca gcggaaggaa gggtggggcg gggcg
//
