LOCUS       sequence-373338-        3790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  M13 packaging signal cloned into the pSEVA191 vector. (This plasmid 
            is a gift of the Jaramillo Lab, where it is called PAJ156.).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Pathania A, Hopper C, Pandi A, Fugger M, Nowak T, Kushwaha M
  TITLE     A synthetic communication system uncovers extracellular immunity 
            that self-limits bacteriophage transmission
  JOURNAL   bioRxiv (2022)
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2022)"
COMMENT     Sequence Label: pSEVA19_M13ps
FEATURES             Location/Qualifiers
     source          1..3790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..23
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        54..88
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        109..184
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      249..343
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        416..507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(508..576,577..1368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1372..1411
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1558..1666
                     /note="incP origin of transfer"
     rep_origin      1702..2290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2191..2210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2444..2461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2476..2616
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2542..2561)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2702..2724
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2718..2909)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(3078..3587)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3273..3294)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3481..3500
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(3690..3776)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agcggataac aatttcacac aggaggccgc ctaggccgcg gccgcgcgaa ttcttgacag
       61 ctagctcagt cctaggtata atgctagcag agaccacttt cggtctctgt tttagagcta
      121 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      181 gtgcttttct gcaggcatgc aagcttgcgg ccgcgtcgtg actgggaaaa ccctggcgac
      241 tagtcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
      301 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccagggg tccccaataa
      361 ttacgattta aattagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt
      421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      481 cttcaataat attgaaaaag gaagagtatg agcattcagc attttcgtgt ggcgctgatt
      541 ccgttttttg cggcgttttg cctgccggtg tttgcgcatc cggaaaccct ggtgaaagtg
      601 aaagatgcgg aagatcaact gggtgcgcgc gtgggctata ttgaactgga tctgaacagc
      661 ggcaaaattc tggaatcttt tcgtccggaa gaacgttttc cgatgatgag cacctttaaa
      721 gtgctgctgt gcggtgcggt tctgagccgt gtggatgcgg gccaggaaca actgggccgt
      781 cgtattcatt atagccagaa cgatctggtg gaatatagcc cggtgaccga aaaacatctg
      841 accgatggca tgaccgtgcg tgaactgtgc agcgcggcga ttaccatgag cgataacacc
      901 gcggcgaacc tgctgctgac gaccattggc ggtccgaaag aactgaccgc gtttctgcat
      961 aacatgggcg atcatgtgac ccgtctggat cgttgggaac cggaactgaa cgaagcgatt
     1021 ccgaacgatg aacgtgatac caccatgccg gcagcaatgg cgaccaccct gcgtaaactg
     1081 ctgacgggtg agctgctgac cctggcaagc cgccagcaac tgattgattg gatggaagcg
     1141 gataaagtgg cgggtccgct gctgcgtagc gcgctgccgg ctggctggtt tattgcggat
     1201 aaaagcggtg cgggcgaacg tggcagccgt ggcattattg cggcgctggg cccggatggt
     1261 aaaccgagcc gtattgtggt gatttatacc accggcagcc aggcgacgat ggatgaacgt
     1321 aaccgtcaga ttgcggaaat tggcgcgagc ctgattaaac attggtaaac cgatacaatt
     1381 aaaggctcct tttggagcct ttttttttgg acgacccttg tccttttccg ctgcataacc
     1441 ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac
     1501 aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg
     1561 caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc
     1621 gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggccccgt
     1681 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1741 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1801 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     1861 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1921 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1981 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2041 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2101 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2161 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2221 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2281 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2341 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2401 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2461 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2521 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     2581 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     2641 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     2701 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     2761 cggtaaagct catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg
     2821 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     2881 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     2941 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     3001 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     3061 gggcgcgaga ttgatttacc ccggttgata atcagaaaag ccccaaaaac aggaagattg
     3121 tataagcaaa tatttaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3181 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3241 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3301 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3361 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3421 accctaaagg gagcccccga tttagagctt gacggggaaa gcgaacgtgg cgagaaagga
     3481 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3541 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtaaa aggatctagg
     3601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3661 gaggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac
     3721 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     3781 ctttaattaa
//
