LOCUS       sequence-373340-        6067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VSV-G envelope expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Susac L, Vuong MT, Thomas C, von Bulow S, O'Brien-Ball C, Santos AM,
            Fernandes RA, Hummer G, Tampe R, Davis SJ
  TITLE     Structure of a fully assembled tumor-specific T cell receptor 
            ligated by pMHC.
  JOURNAL   Cell. 2022 Aug 18;185(17):3201-3213.e19. doi: 
            10.1016/j.cell.2022.07.010.
  PUBMED    35985289
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.07"; journalName: "Cell"; date: "2022-08-18- 
            18"; volume: "185"; issue: "17"; pages: "3201-3213.e19"
COMMENT     Sequence Label: pMDG
FEATURES             Location/Qualifiers
     source          1..6067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     611..631
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     657..681
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          794..1269
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             join(1331..1378,1379..2866)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    3068..3462
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3794..3812)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3880..3984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3985..4053,4054..4845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4203..4222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     5381..5400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca
       61 acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      121 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      721 agacaccggg accgatccag cctcccctcg aagcttacat gtggtaccga gctcggatcc
      781 tgagaacttc agggtgagtc tatgggaccc ttgatgtttt ctttcccctt cttttctatg
      841 gttaagttca tgtcatagga aggggagaag taacagggta cacatattga ccaaatcagg
      901 gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact tttttgttta
      961 tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat acaatgtatc
     1021 atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa ggcaatagca
     1081 atatttctgc atataaatat ttctgcatat aaattgtaac tgatgtaaga ggtttcatat
     1141 tgctaatagc agctacaatc cagctaccat tctgctttta ttttatggtt gggataaggc
     1201 tggattattc tgagtccaag ctaggccctt ttgctaatca tgttcatacc tcttatcttc
     1261 ctcccacagc tcctgggcaa cgtgctggtc tgtgtgctgg cccatcactt tggcaaagaa
     1321 ttctgacact atgaagtgcc ttttgtactt agccttttta ttcattgggg tgaattgcaa
     1381 gttcaccata gtttttccac acaaccaaaa aggaaactgg aaaaatgttc cttctaatta
     1441 ccattattgc ccgtcaagct cagatttaaa ttggcataat gacttaatag gcacagcctt
     1501 acaagtcaaa atgcccaaga gtcacaaggc tattcaagca gacggttgga tgtgtcatgc
     1561 ttccaaatgg gtcactactt gtgatttccg ctggtatgga ccgaagtata taacacattc
     1621 catccgatcc ttcactccat ctgtagaaca atgcaaggaa agcattgaac aaacgaaaca
     1681 aggaacttgg ctgaatccag gcttccctcc tcaaagttgt ggatatgcaa ctgtgacgga
     1741 tgccgaagca gtgattgtcc aggtgactcc tcaccatgtg ctggttgatg aatacacagg
     1801 agaatgggtt gattcacagt tcatcaacgg aaaatgcagc aattacatat gccccactgt
     1861 ccataactct acaacctggc attctgacta taaggtcaaa gggctatgtg attctaacct
     1921 catttccatg gacatcacct tcttctcaga ggacggagag ctatcatccc tgggaaagga
     1981 gggcacaggg ttcagaagta actactttgc ttatgaaact ggaggcaagg cctgcaaaat
     2041 gcaatactgc aagcattggg gagtcagact cccatcaggt gtctggttcg agatggctga
     2101 taaggatctc tttgctgcag ccagattccc tgaatgccca gaagggtcaa gtatctctgc
     2161 tccatctcag acctcagtgg atgtaagtct aattcaggac gttgagagga tcttggatta
     2221 ttccctctgc caagaaacct ggagcaaaat cagagcgggt cttccaatct ctccagtgga
     2281 tctcagctat cttgctccta aaaacccagg aaccggtcct gctttcacca taatcaatgg
     2341 taccctaaaa tactttgaga ccagatacat cagagtcgat attgctgctc caatcctctc
     2401 aagaatggtc ggaatgatca gtggaactac cacagaaagg gaactgtggg atgactgggc
     2461 accatatgaa gacgtggaaa ttggacccaa tggagttctg aggaccagtt caggatataa
     2521 gtttccttta tacatgattg gacatggtat gttggactcc gatcttcatc ttagctcaaa
     2581 ggctcaggtg ttcgaacatc ctcacattca agacgctgct tcgcaacttc ctgatgatga
     2641 gagtttattt tttggtgata ctgggctatc caaaaatcca atcgagcttg tagaaggttg
     2701 gttcagtagt tggaaaagct ctattgcctc ttttttcttt atcatagggt taatcattgg
     2761 actattcttg gttctccgag ttggtatcca tctttgcatt aaattaaagc acaccaagaa
     2821 aagacagatt tatacagaca tagagatgaa ccgacttgga aagtaactca aatcctgcac
     2881 aacagattct tcatgtttgg accaaatcaa cttgtgatac catgctcaaa gaggcctcaa
     2941 ttatatttga gtttttaatt tttatgaaaa aaaaaaaaaa aaancggaat tcaccccacc
     3001 agtgcaggct gcctatcaga aagtggtggc tggtgtggct aatgccctgg cccacaagta
     3061 tcactaagct cgctttcttg ctgtccaatt tctattaaag gttcctttgt tccctaagtc
     3121 caactactaa actgggggat attatgaagg gccttgagca tctggattct gcctaataaa
     3181 aaacatttat tttcattgca atgatgtatt taaattattt ctgaatattt tactaaaaag
     3241 ggaatgtggg aggtcagtgc atttaaaaca taaagaaatg aagagctagt tcaaaccttg
     3301 ggaaaataca ctatatctta aactccatga aagaaggtga ggctgcaaac agctaatgca
     3361 cattggcaac agcccctgat gcctatgcct tattcatccc tcagaaaagg attcaagtag
     3421 aggcttgatt tggaggttaa agttttgcta tgctgtattt tacattactt attgttttag
     3481 ctgtcctcat gaatgtcttt tcactaccca tttgcttatc ctgcatctct cagccttgac
     3541 tccactcagt tctcttgctt agagatacca cctttcccct gaagtgttcc ttccatgttt
     3601 tacggcgaga tggtttctcc tcgcctggcc actcagcctt agttgtctct gttgtcttat
     3661 agaggtctac ttgaagaagg aaaaacaggg ggcatggttt gactgtcctg tgagcccttc
     3721 ttccctgcct cccccactca cagtgacccg gaatccctcg acatggcagt ctagaattct
     3781 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     3841 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3901 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3961 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4021 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4081 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4141 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4201 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     4261 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4321 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4381 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4441 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4501 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     4561 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4621 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4681 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4741 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4801 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4861 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggct
     4921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5461 gggggcggag cctatggaaa aacgccagca acggagatgc gccgcgtgcg gctgctggag
     5521 atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc
     5581 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt
     5641 ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa
     5701 ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat
     5761 aaatcgccgt gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg
     5821 atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
     5881 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
     5941 gcgtcgggga gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     6001 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     6061 tgagctt
//
