LOCUS       sequence-373349-       11126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral packaging plasmid expressing gag and pol..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Susac L, Vuong MT, Thomas C, von Bulow S, O'Brien-Ball C, Santos AM,
            Fernandes RA, Hummer G, Tampe R, Davis SJ
  TITLE     Structure of a fully assembled tumor-specific T cell receptor 
            ligated by pMHC.
  JOURNAL   Cell. 2022 Aug 18;185(17):3201-3213.e19. doi: 
            10.1016/j.cell.2022.07.010.
  PUBMED    35985289
REFERENCE   2  (bases 1 to 11126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.07"; journalName: "Cell"; date: "2022-08-18- 
            18"; volume: "185"; issue: "17"; pages: "3201-3213.e19"
COMMENT     Sequence Label: p8.91
FEATURES             Location/Qualifiers
     source          1..11126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     65..82
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(152..172)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        157..486
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      337..472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          691..756
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             886..906
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     enhancer        1360..1743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1744..1947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1898..1918
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1944..1968
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2222..3724
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVQAGP
                     VAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             3517..6528
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6213..6330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7102..7335
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7520..7564
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7858..7884
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     promoter        complement(8507..8525)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8508..8525)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8815..8834)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9048..9067)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9167..9189
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        9313..9417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9418..9486,9487..10278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9636..9655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10449..11037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10938..10957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
       61 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
      121 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
      181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      241 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      301 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      361 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      421 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      481 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
      541 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
      601 tgcgccagat cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
      661 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
      721 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
      781 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
      841 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
      901 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
      961 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     1021 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     1081 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     1141 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     1201 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     1261 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     1321 tgttgttgtt gggctgcagg aattaattcg agctcgcccg acattgatta ttgactagct
     1381 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1441 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     1501 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1561 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1621 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1681 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1741 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     1801 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     1861 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     1921 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     1981 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg
     2041 gaacggtgca ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga
     2101 gtctataggc ccaccccctt ggcttcttat gcgacggatc gatcccgtaa taagcttcga
     2161 ggtccgcggc cggccgcgtt gacgcgcacg gcaagaggcg aggggcggcc gctggtgaga
     2221 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgatggg aaaaaattcg
     2281 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     2341 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     2401 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     2461 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     2521 tttagacaag atagaggaag agcaaaacaa aagtaagaaa aaagcacagc aagcagcagc
     2581 tgacacagga cacagcaatc aggtcagcca aaattaccct atagtgcaga acatccaggg
     2641 gcaaatggta catcaggcca tatcacctag aactttaaat gcatgggtaa aagtagtaga
     2701 agagaaggct ttcagcccag aagtgatacc catgttttca gcattatcag aaggagccac
     2761 cccacaagat ttaaacacca tgctaaacac agtgggggga catcaagcag ccatgcaaat
     2821 gttaaaagag accatcaatg aggaagctgc agaatgggat agattgcatc cagtgcaggc
     2881 agggcctgtt gcaccaggcc agatgagaga accaagggga agtgacatag caggaactac
     2941 tagtaccctt caggaacaaa taggatggat gacacataat ccacctatcc cagtaggaga
     3001 aatctataaa agatggataa tcctgggatt aaataaaata gtaagaatgt atagccctac
     3061 cagcattctg gacataagac aaggaccaaa ggaacccttt agagactatg tagaccgatt
     3121 ctataaaact ctaagagccg agcaagcttc acaagaggta aaaaattgga tgacagaaac
     3181 cttgttggtc caaaatgcga acccagattg taagactatt ttaaaagcat tgggaccagg
     3241 agcgacacta gaagaaatga tgacagcatg tcagggagtg gggggacccg gccataaagc
     3301 aagagttttg gctgaagcaa tgagccaagt aacaaatcca gctaccataa tgatacagaa
     3361 aggcaatttt aggaaccaaa gaaagactgt taagtgtttc aattgtggca aagaagggca
     3421 catagccaaa aattgcaggg cccctaggaa aaagggctgt tggaaatgtg gaaaggaagg
     3481 acaccaaatg aaagattgta ctgagagaca ggctaatttt ttagggaaga tctggccttc
     3541 ccacaaggga aggccaggga attttcttca gagcagacca gagccaacag ccccaccaga
     3601 agagagcttc aggtttgggg aagagacaac aactccctct cagaagcagg agccgataga
     3661 caaggaactg tatcctttag cttccctcag atcactcttt ggcagcgacc cctcgtcaca
     3721 ataaagatag gggggcaatt aaaggaagct ctattagata caggagcaga tgatacagta
     3781 ttagaagaaa tgaatttgcc aggaagatgg aaaccaaaaa tgataggggg aattggaggt
     3841 tttatcaaag taagacagta tgatcagata ctcatagaaa tctgcggaca taaagctata
     3901 ggtacagtat tagtaggacc tacacctgtc aacataattg gaagaaatct gttgactcag
     3961 attggctgca ctttaaattt tcccattagt cctattgaga ctgtaccagt aaaattaaag
     4021 ccaggaatgg atggcccaaa agttaaacaa tggccattga cagaagaaaa aataaaagca
     4081 ttagtagaaa tttgtacaga aatggaaaag gaaggaaaaa tttcaaaaat tgggcctgaa
     4141 aatccataca atactccagt atttgccata aagaaaaaag acagtactaa atggagaaaa
     4201 ttagtagatt tcagagaact taataagaga actcaagatt tctgggaagt tcaattagga
     4261 ataccacatc ctgcagggtt aaaacagaaa aaatcagtaa cagtactgga tgtgggcgat
     4321 gcatattttt cagttccctt agataaagac ttcaggaagt atactgcatt taccatacct
     4381 agtataaaca atgagacacc agggattaga tatcagtaca atgtgcttcc acagggatgg
     4441 aaaggatcac cagcaatatt ccagtgtagc atgacaaaaa tcttagagcc ttttagaaaa
     4501 caaaatccag acatagtcat ctatcaatac atggatgatt tgtatgtagg atctgactta
     4561 gaaatagggc agcatagaac aaaaatagag gaactgagac aacatctgtt gaggtgggga
     4621 tttaccacac cagacaaaaa acatcagaaa gaacctccat tcctttggat gggttatgaa
     4681 ctccatcctg ataaatggac agtacagcct atagtgctgc cagaaaagga cagctggact
     4741 gtcaatgaca tacagaaatt agtgggaaaa ttgaattggg caagtcagat ttatgcaggg
     4801 attaaagtaa ggcaattatg taaacttctt aggggaacca aagcactaac agaagtagta
     4861 ccactaacag aagaagcaga gctagaactg gcagaaaaca gggagattct aaaagaaccg
     4921 gtacatggag tgtattatga cccatcaaaa gacttaatag cagaaataca gaagcagggg
     4981 caaggccaat ggacatatca aatttatcaa gagccattta aaaatctgaa aacaggaaag
     5041 tatgcaagaa tgaagggtgc ccacactaat gatgtgaaac aattaacaga ggcagtacaa
     5101 aaaatagcca cagaaagcat agtaatatgg ggaaagactc ctaaatttaa attacccata
     5161 caaaaggaaa catgggaagc atggtggaca gagtattggc aagccacctg gattcctgag
     5221 tgggagtttg tcaatacccc tcccttagtg aagttatggt accagttaga gaaagaaccc
     5281 ataataggag cagaaacttt ctatgtagat ggggcagcca atagggaaac taaattagga
     5341 aaagcaggat atgtaactga cagaggaaga caaaaagttg tccccctaac ggacacaaca
     5401 aatcagaaga ctgagttaca agcaattcat ctagctttgc aggattcggg attagaagta
     5461 aacatagtga cagactcaca atatgcattg ggaatcattc aagcacaacc agataagagt
     5521 gaatcagagt tagtcagtca aataatagag cagttaataa aaaaggaaaa agtctacctg
     5581 gcatgggtac cagcacacaa aggaattgga ggaaatgaac aagtagataa attggtcagt
     5641 gctggaatca ggaaagtact atttttagat ggaatagata aggcccaaga agaacatgag
     5701 aaatatcaca gtaattggag agcaatggct agtgatttta acctaccacc tgtagtagca
     5761 aaagaaatag tagccagctg tgataaatgt cagctaaaag gggaagccat gcatggacaa
     5821 gtagactgta gcccaggaat atggcagcta gattgtacac atttagaagg aaaagttatc
     5881 ttggtagcag ttcatgtagc cagtggatat atagaagcag aagtaattcc agcagagaca
     5941 gggcaagaaa cagcatactt cctcttaaaa ttagcaggaa gatggccagt aaaaacagta
     6001 catacagaca atggcagcaa tttcaccagt actacagtta aggccgcctg ttggtgggcg
     6061 gggatcaagc aggaatttgg cattccctac aatccccaaa gtcaaggagt aatagaatct
     6121 atgaataaag aattaaagaa aattatagga caggtaagag atcaggctga acatcttaag
     6181 acagcagtac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     6241 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     6301 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     6361 gtttggaaag gaccagcaaa gctcctctgg aaaggtgaag gggcagtagt aatacaagat
     6421 aatagtgaca taaaagtagt gccaagaaga aaagcaaaga tcatcaggga ttatggaaaa
     6481 cagatggcag gtgatgattg tgtggcaagt agacaggatg aggattaaca catggaattc
     6541 tgcaacaact gctgtttatc catttcagaa ttgggtgtcg acatagcaga ataggcgtta
     6601 ctcgacagag gagagcaaga aatggagcca gtagatccta gactagagcc ctggaagcat
     6661 ccaggaagtc agcctaaaac tgcttgtacc aattgctatt gtaaaaagtg ttgctttcat
     6721 tgccaagttt gtttcatgac aaaagcctta ggcatctcct atggcaggaa gaagcggaga
     6781 cagcgacgaa gagctcatca gaacagtcag actcatcaag cttctctatc aaagcagtaa
     6841 gtagtacatg taatgcaacc tataatagta gcaatagtag cattagtagt agcaataata
     6901 atagcaatag ttgtgtggtc catagtaatc atagaatata ggaaaatggc cgctgatctt
     6961 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     7021 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     7081 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     7141 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     7201 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     7261 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7321 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7381 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7441 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     7501 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     7561 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     7621 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     7681 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     7741 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     7801 agtgaacgga tccttggcac ttatctggga cgatctgcgg agcctgtgcc tcttcagcta
     7861 ccaccgcttg agagacttac tcttgattgt aacgaggatt gtggaacttc tgggacgcag
     7921 ggggtgggaa gccctcaaat attggtggaa tctcctacaa tattggagtc aggagctaaa
     7981 gaatagtgct gttagcttgc tcaatgccac agccatagca gtagctgagg ggacagatag
     8041 ggttatagaa gtagtacaag gagcttgtag agctattcgc cacataccta gaagaataag
     8101 acagggcttg gaaaggattt tgctataagc tcgaggccgc cccggtgacc ttcagacctt
     8161 ggcactggag gtggcccggc agaagcgcgg catcgtggat cagtgctgca ccagcatctg
     8221 ctctctctac caactggaga actactgcaa ctaggcccac cactaccctg tccacccctc
     8281 tgcaatgaat aaaacctttg aaagagcact acaagttgtg tgtacatgcg tgcatgtgca
     8341 tatgtggtgc ggggggaaca tgagtggggc tggctggagt ggcgatgata agctgtcaaa
     8401 catgagaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     8461 tgtcatgata ataatggttt cttagtctag aattaattcc gtgtattcta tagtgtcacc
     8521 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
     8581 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
     8641 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
     8701 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
     8761 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     8821 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     8881 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     8941 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     9001 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
     9061 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     9121 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     9181 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     9241 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     9301 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9361 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9421 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9481 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9541 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9601 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9661 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9721 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9781 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9841 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9901 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9961 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    10021 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    10081 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10141 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10201 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    10261 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    10321 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10381 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10441 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10501 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    10561 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    10621 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10681 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10741 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10801 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10861 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10921 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10981 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11041 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    11101 tttcctgcgt tatcccctga ttctgt
//
