LOCUS       sequence-373363-       11018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG tagged Cas12j3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11018)
  AUTHORS   Escobar M, Li J, Patel A, Liu S, Xu Q, Hilton IB
  TITLE     Quantification of Genome Editing and Transcriptional Control 
            Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in
            Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3239-3250. doi: 
            10.1021/acssynbio.2c00156. Epub 2022 Sep 26.
  PUBMED    36162812
REFERENCE   2  (bases 1 to 11018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00156"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3239-3250"
COMMENT     Sequence Label: Cas12j3
FEATURES             Location/Qualifiers
     source          1..11018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        2071..2146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2208..2419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2438..2447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4742..4789
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4790..4813
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4823..4879
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4880..5476
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5373..5393
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5492..6080
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5545..5565)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5963..5974)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6152..6385
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6411..6428)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6417..6641
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6687..7115
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6774..6793)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6984..7005
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7124..7144)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7129..7458
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7309..7444
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7371..7390
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7506..7553
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7572..7946
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8076..8197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8113..8132)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8167..8186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8246..8262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8246..8262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8259..8281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8270..8286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8294..8300,8301..8318,8319..8324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8339..8360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8477..8494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8648..9236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8728..8747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9407..10198,10199..10267))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10030..10049
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10268..10372)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10447..10466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10638..11017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg atatcactag tgttccatta actgcgctaa aaataatttt taaatctttt
     2041 ttagcttctt gctctttttt gtacgtctct gttttagagc tagaaatagc aagttaaaat
     2101 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc
     2161 tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     2221 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag
     2281 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2341 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2401 gtttgccgcc agaacacagg accggttcta gagcgctgcc accatggaaa aagaaataac
     2461 tgagctcacc aagattaggc gcgagtttcc gaataaaaag ttcagcagca ctgatatgaa
     2521 gaaggcaggt aagttgttga aggcagaagg tcctgatgct gttagagact tcctgaactc
     2581 ctgccaggag attatcgggg attttaagcc gcctgtaaag acaaacatag tcagcatatc
     2641 acgacccttt gaggagtggc ctgttagtat ggtggggcgc gccatccagg aatattactt
     2701 tagtttgaca aaagaggaat tggagtccgt ccatcccgga acttccagcg aggatcacaa
     2761 gtccttcttt aacataactg gcctgagcaa ttacaattat acgtcagtcc aaggcttgaa
     2821 tctcatcttc aaaaatgcga aggccatata cgacgggact ctggttaaag caaacaataa
     2881 aaataagaag ttggaaaaaa agttcaatga gattaaccac aagcgaagcc ttgaggggct
     2941 tcctataatt acgccggatt tcgaggaacc ctttgatgag aatggccatc tgaataatcc
     3001 gccaggtatt aatcgaaata tttacggcta ccaaggatgt gccgctaaag tattcgttcc
     3061 ttccaagcat aaaatggtat ccctccctaa agaatacgaa gggtacaacc gggatccgaa
     3121 cctgtccttg gcgggcttcc gaaatcggct cgagataccg gagggggagc ccggtcacgt
     3181 gccatggttt cagcgcatgg atatcccgga aggccagatc gggcacgtaa ataagattca
     3241 acgattcaat ttcgttcatg gcaagaattc aggaaaagtc aaattcagcg ataagacagg
     3301 acgggtaaaa cgctaccatc attccaagta taaagatgcc actaagcctt acaaatttct
     3361 tgaagaatcc aagaaagtca gtgctctgga ctccatcctt gccattatca caatcggtga
     3421 tgactgggta gtgtttgaca ttcgcggtct gtatagaaat gttttttatc gcgaactggc
     3481 acagaagggc ctgacagcag tgcagctgct ggatctgttt acgggggatc cggtgattga
     3541 cccgaagaag ggcgttgtga cattcagcta taaggaaggc gtggttccag tattttcaca
     3601 gaagatcgtt ccaaggttca agagtcgaga cacgctcgag aaattgacca gtcaaggacc
     3661 tgtggcgctg ctctcagtcg acctcggcca aaatgaacca gtggcggcaa gggtttgtag
     3721 cttgaagaac ataaatgata agatcacatt ggataattct tgcagaatct ccttcctgga
     3781 tgactacaaa aaacaaatca aagactacag agattccctg gacgaacttg aaatcaagat
     3841 acgactggaa gcaatcaatt ctctggaaac taaccaacaa gtagaaattc gcgacctgga
     3901 tgtattcagt gctgatcggg caaaggcaaa cactgtagat atgttcgaca tcgacccaaa
     3961 tttgatatcc tgggattcaa tgagcgacgc gagggtgagc acgcaaataa gcgatcttta
     4021 tctgaagaat gggggtgacg aatctcgagt atatttcgaa attaacaaca aacggataaa
     4081 gcgatctgat tataacatta gtcagctggt gaggccaaag ctttccgaca gcactcggaa
     4141 gaatctgaac gattctatat ggaagttgaa aagaactagt gaagaatatt tgaaattgtc
     4201 caaacgaaag ttggaactga gcagagctgt tgtgaactac actatccgcc agagcaagct
     4261 cctctccgga attaacgaca ttgttataat acttgaggac ctggatgtaa aaaaaaaatt
     4321 caatggcagg ggcattcgag atatcggatg ggacaacttc ttcagctcca ggaaagagaa
     4381 caggtggttc attccggcat tccataaggc tttctcagag ctttcaagca accggggcct
     4441 ctgtgtcatc gaagtcaacc cggcatggac atctgccacc tgtcccgact gcgggttctg
     4501 tagtaaagag aacagagatg gcattaattt tacctgtcgc aagtgcggtg tctcttacca
     4561 cgcggacata gatgttgcca ctcttaatat agcccgggtg gccgttctcg gcaagcctat
     4621 gtccggaccc gccgaccgcg agagactggg cgatactaag aaaccccggg tagcaaggag
     4681 ccgaaagact atgaaacgga aagatattag caatagcacc gttgaggcta tggttacagc
     4741 caagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaag attacaaaga
     4801 cgatgacgat aagggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt
     4861 cgaagagaat cctggaccga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     4921 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     4981 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5041 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5101 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5161 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5221 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca
     5281 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5341 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5401 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5461 caagcccggt gcctgaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     5521 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5581 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5641 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5701 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5761 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5821 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5881 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5941 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6001 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6061 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     6121 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6181 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     6241 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6301 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6361 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     6421 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6481 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6541 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6601 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6661 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6721 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6781 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6841 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6901 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6961 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7021 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7081 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     7141 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7201 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7261 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7321 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7381 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7441 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     7501 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     7561 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     7621 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     7681 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     7741 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     7801 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     7861 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     7921 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     7981 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8041 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     8101 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8161 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     8221 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8281 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8341 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8401 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8461 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8521 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8581 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8641 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8701 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8761 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8821 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8881 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8941 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9001 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9061 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9121 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9181 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9241 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9301 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9361 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9421 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9481 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9541 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9601 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9661 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9721 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9781 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9841 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9901 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9961 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10021 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10081 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10141 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10201 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10261 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10321 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10381 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
    10441 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    10501 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
    10561 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
    10621 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
    10681 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
    10741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
    10801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
    10861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
    10921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
    10981 gcagtacatc tacgtattag tcatcgctat taccatgg
//
