LOCUS       sequence-373373-       10804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human ZMYND8, neomycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Cao Z, Budinich KA, Huang H, Ren D, Lu B, Zhang Z, Chen Q, Zhou Y, 
            Huang YH, Alikarami F, Kingsley MC, Lenard AK, Wakabayashi A, 
            Khandros E, Bailis W, Qi J, Carroll MP, Blobel GA, Faryabi RB, Bernt
            KM, Berger SL, Shi J
  TITLE     ZMYND8-regulated IRF8 transcription axis is an acute myeloid 
            leukemia dependency.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3604-3622.e10. doi: 
            10.1016/j.molcel.2021.07.018. Epub 2021 Aug 5.
  PUBMED    34358447
REFERENCE   2  (bases 1 to 10804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.018"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3604-3622.e10"
COMMENT     Sequence Label: ZMYND8-2A-neo
FEATURES             Location/Qualifiers
     source          1..10804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2149..2214
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5794..5850
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5845..6645
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5905..5924)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6515..6534
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6661..7249
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6714..6734)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7132..7143)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7321..7554
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7632..7753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7669..7688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7723..7742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7793..7928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7948..7967)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7949..7967)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7977..7994)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7977..7993)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7986..8008)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8135..8590
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8222..8241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8432..8453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8616..8720
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8721..8789,8790..9581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8939..8958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9752..10340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10241..10260
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10494..10511
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10628..10649
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10664..10669,10670..10687,10688..10694)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10702..10718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10707..10729
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10726..10742
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10726..10742
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10761..10781
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10763..10781
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgga ctacaaagac
     2161 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagatgcat
     2221 ccacagagct tggctgaaga ggaaataaaa acagaacagg aggtggtaga gggcatggat
     2281 atctctactc gctccaaaga tcctggctct gcagagagaa cagcccagaa aagaaagttc
     2341 cccagccctc cacattcttc caatggccac tcgccgcagg acacatcaac aagccccatt
     2401 aaaaagaaaa agaaacctgg cttactgaac agtaacaata aggagcagtc agaactaaga
     2461 catggtccgt tttactatat gaagcagcca ctcaccacag accctgttga tgttgtaccg
     2521 caggatggac ggaatgattt ctactgctgg gtttgtcacc gggaaggcca agtcctttgc
     2581 tgtgagctct gtccccgggt ttatcacgct aagtgtctga gactgacatc ggaaccagag
     2641 ggggactggt tttgtcctga atgtgagaaa attacagtag cagaatgcat cgagacccag
     2701 agtaaagcca tgacaatgct caccattgaa cagttatcct acctgctcaa gtttgccatt
     2761 cagaaaatga aacagccagg gacagatgca ttccagaagc ccgttccatt ggaacagcac
     2821 cctgactatg cggaatacat cttccatcca atggaccttt gtacattgga aaagaatgcg
     2881 aaaaagaaaa tgtatggctg cacagaagcc ttcctggctg atgcaaagtg gattttgcac
     2941 aactgcatca tttataatgg gggaaatcac aaattgacgc aaatagcgaa agtagtcatc
     3001 aaaatctgtg aacatgagat gaatgaaatc gaagtatgtc cagaatgtta tctagctgct
     3061 tgccaaaaac gagataactg gttttgtgag ccttgtagca atccacatcc tttggtctgg
     3121 gccaaactga aggggtttcc attctggcct gcaaaagctc taagggataa agacgggcag
     3181 gtcgatgccc gattctttgg acaacatgac agggcctggg ttccaataaa taattgctac
     3241 ctcatgtcta aagaaattcc tttttctgtg aaaaagacta agagcatctt caacagtgcc
     3301 atgcaagaga tggaggttta cgtggagaac atccgcagga agtttggggt ttttaattac
     3361 tctccattta ggacacccta cacacccaac agccagtatc aaatgctgct cgatcccacc
     3421 aaccccagcg ccggcactgc caagatagac aagcaggaga aggtcaagct caactttgac
     3481 atgacggcat cccccaagat cctgatgagc aagcctgtgc tgagtggggg cacaggccgc
     3541 cggatttcct tgtcggatat gccgcgctcc cccatgagca caaactcttc tgtgcacacg
     3601 ggctccgacg tggagcagga tgctgagaag aaggccacgt cgagccactt cagtgcgagc
     3661 gaggagtcca tggacttcct ggataagagc acagcttcac cagcctccac caagacggga
     3721 caagcaggga gtttatccgg cagcccaaag cccttctctc ctcaactgtc agctcctatc
     3781 acgacgaaaa cggacaaaac ctccaccacc ggcagcatcc tgaatcttaa cctggatcga
     3841 agcaaagctg agatggattt gaaggagctg agcgagtcgg tccagcaaca gtccacccct
     3901 gttcctctca tctctcccaa gcgccagatt cgtagcaggt tccagctgaa tcttgacaag
     3961 accatagaga gttgcaaagc acaattaggc ataaatgaaa tctcggaaga tgtctatacg
     4021 gccgtagagc acagcgattc ggaggattct gagaagtcag atagtagcga tagtgagtat
     4081 atcagtgatg atgagcagaa gtctaagaac gagccagaag acacagagga caaagaaggt
     4141 tgtcagatgg acaaagagcc atctgctgtt aaaaaaaagc ccaagcctac aaacccagtg
     4201 gagattaaag aggagctgaa aagcacgtca ccagccagcg agaaggcaga ccctggagca
     4261 gtcaaggaca aggccagccc tgagcctgag aaggactttt ccgaaaaggc aaaaccttca
     4321 cctcacccca taaaggataa actgaaggga aaagatgaga cggattcccc aacagtccat
     4381 ttgggcctgg actctgattc agagagcgaa cttgtcatag atttaggaga agaccattct
     4441 gggcgggagg gtcgaaaaaa taagaaggaa cccaaagaac catctcccaa acaggatgtt
     4501 gtaggtaaaa ctccaccatc cacgacggtg ggcagccatt ctcccccgaa aacaccggtg
     4561 ctcacccgct cttccgccca aacttccgcg gctggcgcca cagccaccac cagcacgtcc
     4621 tccacggtca ccgtcacggc cccggccccc gccgccacag gaagcccagt gaaaaagcag
     4681 aggccgcttt taccgaagga gactgccccg gccgtgcagc gggtcgtgtg gaactcatca
     4741 actgtccagc agaaggagat cacacagagc ccatccacgt ccaccatcac cctggtgacc
     4801 agcacacagt catcgcccct ggtcaccagc tcggggtcca tgagcaccct tgtgtcctca
     4861 gtcaacgctg acctgcccat cgccactgcc tcagctgatg tcgccgctga tattgccaag
     4921 tacactagca aaatgatgga tgcaataaaa ggaacaatga cagaaatata caacgatctt
     4981 tctaaaaaca ctactggaag cacaatagct gagattcgca ggctgaggat cgagatagag
     5041 aagctccagt ggctgcacca gcaagagctc tccgaaatga aacacaactt agagctgacc
     5101 atggcggaga tgcggcagag cctggagcag gagcgggacc ggctcatcgc cgaggtgaag
     5161 aagcagctgg agttggagaa gcagcaggcg gtggatgaga ccaagaagaa gcagtggtgc
     5221 gccaactgca agaaggaggc catcttttac tgctgttgga acaccagcta ctgtgactac
     5281 ccctgccagc aagcccactg gcctgagcac atgaagtcct gcacccagtc agctactgct
     5341 cctcagcagg aagcggatgc tgaggtgaac acagaaacac taaataagtc ctcccagggg
     5401 agctcctcga gcacacaatc agcaccttca gaaacggcca gcgcctccaa agagaaggag
     5461 acgtcagctg agaaaagcaa ggagagtggc tcgacccttg acctttctgg ctccagagag
     5521 acgccctcct ccattctctt aggctccaac caaggctctg ttagcaaaag gtgtgacaag
     5581 caacctgcct atgccccaac caccacagac caccagccgc accccaacta ccccgcccag
     5641 aagtaccatt cccggagtaa taaatccagt tggagcagca gtgatgagaa gaggggatcg
     5701 acacgttccg atcacaacac cagtaccagc acgaagagcc tcctcccgaa agagtctcgg
     5761 ctggacacct tctgggacgc tagcggcagc ggcgccacca acttcagcct gctgaagcag
     5821 gccggcgacg tggaggagaa ccccggcccc atgattgaac aagatggatt gcacgcaggt
     5881 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5941 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6001 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6061 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6121 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6181 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6241 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6301 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6361 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6421 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6481 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6541 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6601 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaacgcg ttaagtcgac
     6661 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6721 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6781 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6841 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6901 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6961 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7021 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7081 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7141 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7201 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     7261 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7321 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7381 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7441 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7501 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     7561 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7621 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7681 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7741 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     7801 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7861 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7921 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7981 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8041 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8101 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     8161 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8221 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8281 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8341 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8401 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8461 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8521 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8581 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8641 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8701 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8761 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8821 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8881 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8941 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     9001 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9061 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9121 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9181 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9241 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9301 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9361 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9421 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9481 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9541 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9601 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9661 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9721 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9781 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9841 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9901 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9961 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10021 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10081 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10141 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10201 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10261 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10321 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10381 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10441 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10501 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    10561 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    10621 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    10681 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    10741 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    10801 aagc
//
