LOCUS       sequence-373422-        3075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone with ORF encoding PH domain of human phospholipase C 
            delta 1 flanked by Gateway recombination sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3075)
  TITLE     Lemaire Lab Plasmids 2022
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENT-L1-Kozak-PH-domain-L2
FEATURES             Location/Qualifiers
     source          1..3075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     212..229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(388..415)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(507..593)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     642..664
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     656..673
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     657..673
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    689..788
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1320..1419)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1436..1455)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1437..1455)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1460..1476)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1460..1476)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1578..1597)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1589..2398
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1656..1675)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(2545..3075,1..58)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3034..3053
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      121 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      181 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      241 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      301 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      361 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      421 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      481 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      541 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      601 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      661 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      721 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      781 agcaggctca gaaaaaatgg actcgggccg ggacttcctg accctgcacg gcctacagga
      841 tgatgaggat ctacaggcgc tgctgaaggg cagccagctc ctgaaggtga agtccagctc
      901 atggaggaga gagcgcttct acaagttgca ggaggactgc aagaccatct ggcaggagtc
      961 ccgcaaggtc atgcggaccc cggagtccca gctgttctcc atcgaggaca ttcaggaggt
     1021 gcgaatgggg caccgcacgg agggtctgga gaagttcgcc cgtgatgtgc ccgaggaccg
     1081 ctgcttctcc attgtcttca aggaccagcg caatacacta gacctcatcg ccccatcgcc
     1141 agctgatgcc cagcactggg tgctggggct gcacaagatc atccaccact caggctccat
     1201 ggaccagcgt cagaagctac agcactggat tcactcctgc ttgcgaaaag ctgacaaaaa
     1261 caaggacaac aagatgagct tcaaggagct gcagaacttc ctgaaggagc tcaacatcaa
     1321 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     1381 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     1441 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     1501 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     1561 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     1621 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     1681 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     1741 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     1801 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     1861 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     1921 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     1981 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     2041 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     2101 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     2161 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     2221 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     2281 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     2341 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     2401 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     2461 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     2521 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2581 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2641 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2701 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2761 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2821 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2881 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2941 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3001 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3061 tgtcgggttt cgcca
//
