LOCUS       sequence-373433-        3468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked 
            by Gateway recombination sequences. No Stop sequence: to be 
            recombined into a destination vector with a C-terminal protein tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero 
            C, Mueller F, Lemaire P
  TITLE     A multicassette Gateway vector set for high throughput and 
            comparative analyses in ciona and vertebrate embryos.
  JOURNAL   PLoS One. 2007. 2(9):e916.
  PUBMED    17878951
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2007. 2(9):e916."
COMMENT     Sequence Label: pENT-L1-Kozak-Ark-L2
FEATURES             Location/Qualifiers
     source          1..3468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     103..120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(279..306)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(398..484)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     533..555
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     547..564
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     548..564
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    580..679
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1604..1703)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1720..1739)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1721..1739)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1744..1760)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1744..1760)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1862..1881)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1873..2682
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1940..1959)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2829..3417
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3318..3337
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
       61 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      121 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      181 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      241 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
      301 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
      361 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
      421 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
      481 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
      541 cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga
      601 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
      661 ttgtacaaaa aagcaggctc agaaaaaatg agttctgcca acaatgaaaa caaagctcct
      721 gagaaagctt caacatctgg tcgtgctact gctagttccg ccaaagataa tgcaagtgac
      781 acttggtcac ttaaaaactt tgacattggc aaaccactgg gacgaggaaa gtttggcagc
      841 gtgtaccttg ctagagagaa aaagagcaag tttatcgttg cactgaaagt gctgtttaaa
      901 tcgcaactca tgaaaagtaa tgtggagcat caattacgaa gagagattga aattcagtct
      961 catttgcgtc atccacacat tctgcgactt tacggttact ttcacgatga gacgagagtg
     1021 tatctgatcc tggaatatgc atctcgtggg gaaatgtaca aggagctgca gaagcagggc
     1081 aagtttacag aggagatgtc cgccacgtac atagccgagc tagcagatgc gctcaactac
     1141 tgccacagca aacaagtcat tcatcgtgac atcaaacctg agaacttgtt gatgggtctt
     1201 cgaggggaat tgaagattgc tgattttggt tggtctgtgc atgctccgtc ctctaaacgc
     1261 caaactcttt gtggtacgct tgattacctc ccaccagaga tgattgaagc aaaagatcat
     1321 gacgctaacg tcgacctgtg gacacttggg attctatgct atgagtttct tgttggcaaa
     1381 ccaccttttg aaacaaaaag cacacaagaa acatacctca gaattacatc actgaaatat
     1441 gcattccctc cccatgtatc agagggagct cgtgatctta ttcgtcgcct gttaaagttg
     1501 gaaccacgac accgacttcc actggaaagt gtaatggctc acccctggat caaagccaat
     1561 gccaaagtac ataagtttgg accagatggt cgacctatgg acaacccagc tttcttgtac
     1621 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     1681 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     1741 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     1801 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1861 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     1921 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1981 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     2041 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     2101 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     2161 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     2221 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     2281 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     2341 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2401 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2461 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2521 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2581 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2641 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     2701 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     2761 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2821 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2881 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2941 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3001 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3061 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3121 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3181 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3241 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3301 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3361 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3421 gccagcaacg cggccttttt acggttcctg gccttttgct ggcctttt
//
