LOCUS       sequence-373437-        3436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked
            by Gateway recombination sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero 
            C, Mueller F, Lemaire P
  TITLE     A multicassette Gateway vector set for high throughput and 
            comparative analyses in ciona and vertebrate embryos.
  JOURNAL   PLoS One. 2007. 2(9):e916.
  PUBMED    17878951
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2007. 2(9):e916."
COMMENT     Sequence Label: pENT-L3-pSnail-L5
FEATURES             Location/Qualifiers
     source          1..3436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..24
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(183..210)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(302..388)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     437..459
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     451..468
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     452..468
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    491..586
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1473..1568)
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1592..1611)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1593..1611)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1616..1632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1616..1632)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1734..1753)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1745..2554
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1812..1831)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2701..3289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3190..3209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
       61 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      121 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
      181 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
      241 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
      301 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      361 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      421 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
      481 cccctctaga aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
      541 gatgagcaat gcttttttat aatgccaact ttgtataata aagtaggctc atgataaatt
      601 aatatgttgt taccgataaa ttgaagttat tcaaaatgaa aacgttgtaa ttgtatttac
      661 gattattaag cagaagatcg gtgtgttttg ctgggcaaat attgagaata aaaattgaga
      721 aaatctgtgg cgacaactgt ggtctgattt acaggcgatt tggacagagt tgacaggtcg
      781 cgaaagcgat cccaccttcg tgtctgggtt tcgaggtgcc gcccgttgtg cagtagcaac
      841 tgtgcggtca agttgagagc aagaagacac cacaacgcgc tttgaagcgt ccaagccgcc
      901 cgctgagctg ggcggatagc gcgtttggca cctcgtctgg catcgtccgc actgtcaacc
      961 ttcttcgtac aaacgtcctc acctgccgcg caggattgct tcatatggca gaccgagttg
     1021 agtgggaaat catattctga ttgcttcgct tatagagcta gctcactaga gactagagtg
     1081 tcgtgaatca tttgaattaa gctgcatagt ttagttgatc aaatgagtct tagttcgatg
     1141 tgcaggactg ctaagtaata cagttaatat agagttattt aaagtgagtt tatgtttaac
     1201 tcttatgtta aacagcatag gacaatttag atagacctat atattataca gaaaataaga
     1261 tagaaaaaat acgagtgcat aaacttacag cacgcttatt gaaaaaggca gtctaacaaa
     1321 ataggaacat ttaaggtatt tgaaagatta ttgcaagttt atttcattag tattattttt
     1381 ttagactttt ggtacatatt caaaatatca aaattttcac tgtgaaatat attactgtga
     1441 ttacatcatg acctccgtcg agcccatgca cccaactttt gtatacaaag ttggcattat
     1501 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     1561 cattatttgc catccagctc tagatgatat cccctatagt gagtcgtatt acatggtcat
     1621 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     1681 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     1741 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     1801 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     1861 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     1921 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     1981 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2041 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2101 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2161 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2221 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2281 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2341 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     2401 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     2461 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     2521 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     2581 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     2641 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2701 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2761 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2821 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2881 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2941 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3001 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3061 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3121 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3181 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3241 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3301 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3361 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3421 ccgcagccga acgacc
//
