LOCUS       sequence-373440-        3496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone with ORF encoding Titf1 cis-regulatory region flanked by
            Gateway recombination sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero 
            C, Mueller F, Lemaire P
  TITLE     A multicassette Gateway vector set for high throughput and 
            comparative analyses in ciona and vertebrate embryos.
  JOURNAL   PLoS One. 2007. 2(9):e916.
  PUBMED    17878951
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2007. 2(9):e916."
COMMENT     Sequence Label: pENT-L3-Titf-L4
FEATURES             Location/Qualifiers
     source          1..3496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     190..207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(366..393)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(485..571)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     620..642
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     634..651
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     635..651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    674..769
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1722..1817)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1835..1854)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1836..1854)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1859..1875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1859..1875)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1977..1996)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1988..2797
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2055..2074)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(2944..3496,1..36)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3433..3452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
       61 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      121 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      181 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      241 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      301 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
      361 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
      421 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
      481 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      541 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
      601 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
      661 gggcccctct agaaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
      721 attgatgagc aatgcttttt tataatgcca actttgtata ataaagtagg ctcaccgctc
      781 gatggggctc aagtgctctt ctcgctgaac ccgagcccgg gaactgacgg aacattttca
      841 aagttcttct gcgacagatg atttcaggaa taattgtttg atagttcgcg gaatgcgagg
      901 ctacagctgc cgccggatat tttccaaacg ttgtttgttt taagctccca tggcgaacta
      961 agcttcaagc ctttcagcat tcgctccttt gacactaaaa attatttcca aaaccagagc
     1021 agctttcatg gaaccgaatt tcttgttttc cagaataaat gtcgttttcc agaataaaat
     1081 ttgatttttt tggaataata catagttctt ccagataaaa tatcattttc ctcgactaaa
     1141 gtgtcatttt ttcaaaataa aatgtcgctt cttacgaatg aaatcttcgt atacaatgcg
     1201 tatactgtat cctgcatttg attccttatc tttctggtta gttgtgtctg tgcagacaca
     1261 tctgtcgaaa ataacttcaa acgcttatca gcgctaatga gtttgtaatg tcgcatttag
     1321 tttggaacgg aaagttccga ttcgtatttg cttttgattt ccgttcgtta gaccgtttca
     1381 tttcccgctc gatgcggctg ccgaccacag tctgtgacgt cacaagttca attgttccat
     1441 ggctgaaata aagtttaaaa tatatagtgg ggtgggggaa gatgggatac cttttcattc
     1501 tactttctcg tccgatttgg tagtaaacaa agaacattca aagaattata aaaccgtatc
     1561 ctcacggctc ctacagaccg ttgttaattg tttaaaacac ggtcaggata tttggatatt
     1621 atgtgctaag ggtgtcccat cttcccccac cctactataa cagtttaaat agtaccattc
     1681 aaggacagat tggacaagtt tggcctgcgg gttaaaatac ccaacttttc tatacaaagt
     1741 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     1801 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     1861 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     1921 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     1981 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     2041 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     2101 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     2161 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     2221 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     2281 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     2341 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     2401 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     2461 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     2521 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     2581 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     2641 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     2701 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     2761 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     2821 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     2881 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3481 acttgagcgt cgattt
//
