LOCUS       sequence-373447-       15027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Yu G, Kim HK, Park J, Kwak H, Cheong Y, Kim D, Kim J, Kim J, Kim HH
  TITLE     Prediction of efficiencies for diverse prime editing systems in 
            multiple cell types.
  JOURNAL   Cell. 2023 Apr 21:S0092-8674(23)00331-8. doi: 
            10.1016/j.cell.2023.03.034.
  PUBMED    37119812
REFERENCE   2  (bases 1 to 15027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Apr 21:S0092-8674(23)00331-8. doi: 10.1016/j.cell.2023.03.034."
COMMENT     Sequence Label: pLenti-NRCH-PE2-BSD
FEATURES             Location/Qualifiers
     source          1..15027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             119..299
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(325..342)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    331..555
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      601..1029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(688..707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     898..919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1038..1058)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1203..1337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1264..1283
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1399..1446
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1465..1839
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1969..2090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2006..2025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2060..2079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2139..2155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2139..2155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2152..2174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2163..2179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2187..2193,2194..2211,2212..2217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2232..2253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2370..2387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2541..3129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2621..2640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3300..4091,4092..4160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3923..3942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4161..4265)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4340..4359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4531..4910
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4912..5110
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5065..5085
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5128..5308
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5355..5480
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5973..6206
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6391..6435
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6733..6850
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6943..7154
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             7205..7225
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7226..11326
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMVKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGIIPHQIHLGELHAILRRQGDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRLRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKGNSDKLIARKKDWDPKKYGGFNSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTINRKQYNTTKEVLD
                     ATLIRQSITGLYETRIDLSQLGGD"
     CDS             13499..13519
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13520..13543
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             13553..13609
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13610..14008
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    14033..14621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14086..14106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14504..14515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(14624..14640)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14625..14641)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
       61 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
      121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      301 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      361 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      421 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      481 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
      541 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
      601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1021 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     1081 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     1141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     1201 agtcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     1261 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     1321 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     1381 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     1441 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     1501 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     1561 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     1621 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     1681 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     1741 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     1801 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     1861 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     1921 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     1981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     2101 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     2161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2221 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4261 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     4321 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     4381 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     4441 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     4501 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     4561 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     4621 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     4681 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     4741 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     4801 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     4861 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     4921 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     4981 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     5041 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     5101 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5161 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5221 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5281 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     5341 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     5401 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     5461 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     5521 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     5581 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     5641 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     5701 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     5761 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     5821 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     5881 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     5941 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     6001 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     6061 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     6121 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     6181 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     6241 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     6301 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     6361 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     6421 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     6481 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     6541 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     6601 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     6661 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
     6721 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     6781 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     6841 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttagctagct
     6901 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     6961 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
     7021 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     7081 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     7141 ccgccagaac acaggaccgg ttgccaccat gaaacggaca gccgacggaa gcgagttcga
     7201 gtcaccaaag aagaagcgga aagtcgacaa gaagtacagc atcggcctgg acatcggcac
     7261 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
     7321 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
     7381 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
     7441 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
     7501 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
     7561 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
     7621 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
     7681 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
     7741 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
     7801 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
     7861 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
     7921 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
     7981 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
     8041 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
     8101 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
     8161 caaggccccc ctgagcgcct ctatggtgaa gagatacgac gagcaccacc aggacctgac
     8221 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
     8281 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
     8341 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
     8401 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcatca tcccccacca
     8461 gatccacctg ggagagctgc acgccattct gcggcggcag ggcgattttt acccattcct
     8521 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     8581 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     8641 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     8701 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     8761 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     8821 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     8881 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     8941 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     9001 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     9061 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     9121 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     9181 gctgagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     9241 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     9301 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     9361 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     9421 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggcgg
     9481 ccacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     9541 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     9601 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     9661 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     9721 gtccgactac gatgtggacg ctatcgtgcc tcagagcttt ctgaaggacg actccatcga
     9781 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     9841 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     9901 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     9961 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
    10021 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
    10081 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
    10141 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
    10201 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
    10261 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
    10321 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
    10381 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
    10441 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
    10501 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa
    10561 gggcaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
    10621 cttcaacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
    10681 gtccaagaaa ctgaagagtg tgaaagagct actggggatc accatcatgg aaagaagcag
    10741 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
    10801 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
    10861 gctggcctct gccggcgtgc tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
    10921 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
    10981 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
    11041 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
    11101 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
    11161 taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcaaccg
    11221 gaagcagtac aacaccacca aagaggtgct ggacgccacc ctgatccggc agagcatcac
    11281 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgactctg gaggatctag
    11341 cggaggatcc tctggcagcg agacaccagg aacaagcgag tcagcaacac cagagagcag
    11401 tggcggcagc agcggcggca gcagcaccct aaatatagaa gatgagtatc ggctacatga
    11461 gacctcaaaa gagccagatg tttctctagg gtccacatgg ctgtctgatt ttcctcaggc
    11521 ctgggcggaa accgggggca tgggactggc agttcgccaa gctcctctga tcatacctct
    11581 gaaagcaacc tctacccccg tgtccataaa acaatacccc atgtcacaag aagccagact
    11641 ggggatcaag ccccacatac agagactgtt ggaccaggga atactggtac cctgccagtc
    11701 cccctggaac acgcccctgc tacccgttaa gaaaccaggg actaatgatt ataggcctgt
    11761 ccaggatctg agagaagtca acaagcgggt ggaagacatc caccccaccg tgcccaaccc
    11821 ttacaacctc ttgagcgggc tcccaccgtc ccaccagtgg tacactgtgc ttgatttaaa
    11881 ggatgccttt ttctgcctga gactccaccc caccagtcag cctctcttcg cctttgagtg
    11941 gagagatcca gagatgggaa tctcaggaca attgacctgg accagactcc cacagggttt
    12001 caaaaacagt cccaccctgt ttaatgaggc actgcacaga gacctagcag acttccggat
    12061 ccagcaccca gacttgatcc tgctacagta cgtggatgac ttactgctgg ccgccacttc
    12121 tgagctagac tgccaacaag gtactcgggc cctgttacaa accctaggga acctcgggta
    12181 tcgggcctcg gccaagaaag cccaaatttg ccagaaacag gtcaagtatc tggggtatct
    12241 tctaaaagag ggtcagagat ggctgactga ggccagaaaa gagactgtga tggggcagcc
    12301 tactccgaag acccctcgac aactaaggga gttcctaggg aaggcaggct tctgtcgcct
    12361 cttcatccct gggtttgcag aaatggcagc ccccctgtac cctctcacca aaccggggac
    12421 tctgtttaat tggggcccag accaacaaaa ggcctatcaa gaaatcaagc aagctcttct
    12481 aactgcccca gccctggggt tgccagattt gactaagccc tttgaactct ttgtcgacga
    12541 gaagcagggc tacgccaaag gtgtcctaac gcaaaaactg ggaccttggc gtcggccggt
    12601 ggcctacctg tccaaaaagc tagacccagt agcagctggg tggccccctt gcctacggat
    12661 ggtagcagcc attgccgtac tgacaaagga tgcaggcaag ctaaccatgg gacagccact
    12721 agtcattctg gccccccatg cagtagaggc actagtcaaa caaccccccg accgctggct
    12781 ttccaacgcc cggatgactc actatcaggc cttgcttttg gacacggacc gggtccagtt
    12841 cggaccggtg gtagccctga acccggctac gctgctccca ctgcctgagg aagggctgca
    12901 acacaactgc cttgatatcc tggccgaagc ccacggaacc cgacccgacc taacggacca
    12961 gccgctccca gacgccgacc acacctggta cacggatgga agcagtctct tacaagaggg
    13021 acagcgtaag gcgggagctg cggtgaccac cgagaccgag gtaatctggg ctaaagccct
    13081 gccagccggg acatccgctc agcgggctga actgatagca ctcacccagg ccctaaagat
    13141 ggcagaaggt aagaagctaa atgtttatac tgatagccgt tatgcttttg ctactgccca
    13201 tatccatgga gaaatataca gaaggcgtgg gtggctcaca tcagaaggca aagagatcaa
    13261 aaataaagac gagatcttgg ccctactaaa agccctcttt ctgcccaaaa gacttagcat
    13321 aatccattgt ccaggacatc aaaagggaca cagcgccgag gctagaggca accggatggc
    13381 tgaccaagcg gcccgaaagg cagccatcac agagactcca gacacctcta ccctcctcat
    13441 agaaaattca tcaccctctg gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc
    13501 caagaagaag aggaaagtcg attacaaaga cgatgacgat aagggatccg gcgcaacaaa
    13561 cttctctctg ctgaaacaag ccggagatgt cgaagagaat cctggaccga tggccaagcc
    13621 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
    13681 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
    13741 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
    13801 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
    13861 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
    13921 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
    13981 gccctctggt tatgtgtggg agggctaaga attcgatatc aagcttatcg gtaatcaacc
    14041 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
    14101 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    14161 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    14221 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    14281 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    14341 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    14401 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
    14461 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    14521 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    14581 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
    14641 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
    14701 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
    14761 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    14821 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
    14881 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
    14941 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
    15001 agccaatgaa ggagagaaca cccgctt
//
