LOCUS       sequence-373448-        2828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone with ORF encoding Strong basal promoter of FOG flanked 
            by Gateway recombination sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero 
            C, Mueller F, Lemaire P
  TITLE     A multicassette Gateway vector set for high throughput and 
            comparative analyses in ciona and vertebrate embryos.
  JOURNAL   PLoS One. 2007. 2(9):e916.
  PUBMED    17878951
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2007. 2(9):e916."
COMMENT     Sequence Label: pENT-R4-bpFOG-L5
FEATURES             Location/Qualifiers
     source          1..2828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(466..493)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(585..671)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     720..742
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     734..751
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     735..751
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(801..924)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1148..1243)
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1267..1286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1268..1286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1291..1307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1291..1307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1409..1428)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1420..2229
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1487..1506)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(2376..2828,1..136)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
       61 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      361 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      421 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
      481 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
      541 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
      601 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
      661 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
      721 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc ggggccctac aggtcactaa
      781 taccatctaa gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag
      841 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      901 gttcaacttt tctatacaaa gtggcaaagc ttcgtgtatt gtaccggccc attgtcaatc
      961 atgcaaactt gatattatat tgacaagaga agaaggcagt ttaaattaaa actctaaagt
     1021 agagagacat taatctcagc tgacaaggca ggtggtcaca gtaagttcat ttaaatagtt
     1081 ggccaacaat agcctttcca agaaagtatt tttgttccag gtctatacaa aaataacaca
     1141 cataacccaa cttttgtata caaagttggc attataagaa agcattgctt atcaatttgt
     1201 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctctagat
     1261 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1321 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1381 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1441 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1501 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1561 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1621 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1681 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1741 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1801 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1861 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1921 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1981 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2041 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2101 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2161 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2221 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2281 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2341 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2401 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2461 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2521 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2581 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2641 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2701 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2761 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2821 agcggcag
//
