LOCUS       sequence-373458-       12595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) 
            in Drosophila S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Kingston ER, Blodgett LW, Bartel DP
  TITLE     Endogenous transcripts direct microRNA degradation in Drosophila, 
            and this targeted degradation is required for proper embryonic 
            development.
  JOURNAL   Mol Cell. 2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 
            10.1016/j.molcel.2022.08.029.
  PUBMED    36150386
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 
            10.1016/j.molcel.2022.08.029."
COMMENT     Sequence Label: HA-dora[B]_rescue_construct
FEATURES             Location/Qualifiers
     source          1..12595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1650..1676
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1689..1715
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1728..1754
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8166..8219
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8244..8843
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8244..8263)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8740..8760
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8851..8985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8875..8894)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8929..8948
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9586..10174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9666..9685)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10345..11136,11137..11205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10968..10987
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11206..11310)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11378..11396
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_RNA        complement(11548..11623)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggcgtggcgg ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc
       61 atgtgtgcgt gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat
      121 gctgcgctct ctctagcgcc atctcgctta cgcatgctca acgcaccgca tgttgccgtt
      181 tccttttatg cgtcattttg gctcgaaata ggcaattatt taaacaaaga ttagtcaacg
      241 aaaacgctaa aataaataag tctacaatat ggttacttat tgccatgtgt gtgcagccaa
      301 cgatagcaac aaaagcaaca acacaggtgg ctttccctct ttcacttttt gtttgcaagc
      361 cgcgtgcgag caagacggca cgaccggcaa acgcaattac gctgacaaag agcagacgaa
      421 gttttggcga aaaacatcaa ggcgcctgat acgaatgcat ttgcaataac aattgcgata
      481 tttaatattg tttatgaagc tgtttgactt caaaacacac aaaaaaaaaa ataaaacaaa
      541 ttatttgaaa gagaattagg aatcggacgc ttatcgttag ggtaacaaca agaaatgctt
      601 actgagtcac agcctctgga aaactgccgc aagccagaga gagagagaaa aagagggaga
      661 gcagcttaga ccgcatgtgc ttgtgtgtga ggcgtctctc tcttcgtctc tgttgcgcaa
      721 acgcatagac tgcactgaaa aaatcgatta cctatttttt atgaatgaat atttgcacta
      781 ttactattca aaactattaa gatagcaatc acattcaata gccaaatact ataccacctg
      841 agcgatgcaa cgaaatgatc aatttgagca aaaatgctgc atatttagga cggcatcatt
      901 atagaaatgc ttcttgctgt gtacttttct ctcgtctggc agctgtttcg ccgttattgt
      961 taaaaccggc ttaagttagg tgtgttttct acgactagtg aatgccctac tagaagatgt
     1021 gtgttgcaca aaatgtccct ggaataacca atttgaagtg cagatagcag taaacgtaag
     1081 ctaatatgaa tattatttaa ctgtaatgtt ttaatatcgc tggacattac taataaaccc
     1141 actataaaca catgtacata tgtatgtttt ggcatacaat gagtagttgg ggaaaaaatg
     1201 tgtaaaagca ccgtgaccat cacagcataa agataaccag ctgaagtatc gaatatgagt
     1261 aacccccaaa ttgaatcaca tgccgcaact gataggaccc atggaagtac actcttcatg
     1321 gcgatataca agacacacac aagcacgaac acccagttgc ggaggaaatt ctccgtaaat
     1381 gaaaacccaa tcggcgaaca attcataccc atatatggta aaagttttga acgcgacttg
     1441 agagcggaga gcattgcggc tgataaggtt ttagcgctaa gcgggcttta taaaacgggc
     1501 tgcgggacca gttttcatat cactaccgtt tgagttcttg tgctgtgtgg atactcctcc
     1561 cgacacaaag ccgctccatc agccagcagt cgtctaatcc agagaccccg gatcggggta
     1621 cctactagtc cagtgtggtg gaattcatgt atccatatga tgttccagat tatgctggag
     1681 gttcttcata cccctatgat gtgcctgact acgctggcag cgccggatac ccttatgatg
     1741 tgcctgatta tgctggaggg agcggcggaa gtgggagttc aggaggctcc ggatcaagcg
     1801 gcagtagtgg tggaagcgac cgcttcagct tcgacgactc catccgcttc gaggaagact
     1861 ccctctgcag ctggagctct gaaccggaat cgctgtgcaa caactggcgc ggctggaaga
     1921 agcccgctgc aggcagcggc gcaggcaatg gcggcttgag cggcatgggc ggtatggctg
     1981 tggccggcgg ctcaggagca ccgccgttcg gaggagcagg agcctctggt tcctgcacac
     2041 cgggcggcaa tggaacggcc atggccaaca catccacctg cggcagacat tatgaagtat
     2101 ccacattggc cgaactggca gcccgctgtg tggcctccta cataccgttc gagctggttg
     2161 agcacgtgta tccgccggtg ccggagcagc tgcagctgcg cattgcgttc tggagctttc
     2221 cggacaacga ggaggacatc cggctctact cctgtctggc caatagctcg gcggacgagt
     2281 tcaatcgcgg cgactcgttg tttcggttgc gagctgtcaa ggatccgctg cagataggct
     2341 ttcacctctc ggccagcgtt gtcaatcaga cgccgcgtgc ctatttcaat gtggccgtca
     2401 cctttgaccg ccgacgcatc tcctcttgca actgcacctg cacctcgtcc gcctactggt
     2461 gctcccacgt agtcgccgtt tgtctgcatc gcatccattg cccccaggag gtctgtctgc
     2521 gtgccccggt atcggaatca ttgactcgcc tgcaacgcga ccaattgcaa aagtttgccc
     2581 agtatctgat cagcgagctg cctcagcaga tcttgccgac ggctcagcgg ctcctggatg
     2641 agttgctcag tgcgcaaccc acggcgatca acactgtgtg cggtgcacct gatcccatgg
     2701 ctggggcctc gatcaacgac cagaccagct ggtatctgga cgagaagacg ttgcataaca
     2761 acattaaacg aatcctcatc aagttctgtt tgcccgcccc gattgtgttt agcgacgtaa
     2821 actatctgac gaactcggct cccccagcgg cggccgaatg gagctcgcta ctccgaccgc
     2881 tgcggggacg tgagccggag ggcatgtgga atctactttc gatcgtacgc gagatgtaca
     2941 ggcgctgtga tcggaacgca gtgcgtctgc tggagatcat cacggaggag tgcttgtact
     3001 gcgatcagat actcatctgg tggttccaga cgaagttggc actgatgatg gggtcccacg
     3061 gccactccgg cggtaagcat tctaacactc actccaactc cacggcactg cagcatgctt
     3121 gcagttcgct gtgcgacgaa atcgtggcac tgtggcgatt ggcagcgctc aatcccggcc
     3181 tggcgccgga tgagcgagat atgctgcacg ctcagttcac ggcctggcat ctaaagattc
     3241 ttgaccgggt ggtcaagagc cgaatgatgc cgtcgtacac caacaagcac cagcagaact
     3301 cgcgatccga gacggagctg tttatcggct tcaagccggc catcgaggcg tgctacttgg
     3361 actgggaggg ctaccccata ccgggagtga cgcacaccca cgacacgaat cccatctact
     3421 actcgccgtt cacttgcttc aagcacacgg acctcaaggg cgagtctaac aatcccggcc
     3481 agctgaatgc cacccaagcg ctgatgtcga acaacaagca ctacaattac tttagttcat
     3541 ccagcgatca tggcatgcac gcaggagcct tcaagcagag gttggaccgg ccttttcgtg
     3601 agtcgcgatt ctatacgaat ccggccgatc tggacggagg agcagcgttg gcgggcggag
     3661 gatccggtag gttttccacg ggactgggct ccgttgccgt tggaggagct ggtgcaggag
     3721 gcttgggtca gatgagcgga ggagttaaca actctgttgg aggatcagcg gccgccgaag
     3781 tgaatcccgt gcagcagcag cttcccagtg gctcggcggg agtcggtgcc tctgtgacaa
     3841 atgccaaggt tgtggtagca acagatggca atcgttcgag tgccagcagc gagggattct
     3901 gcgagaacga cgatttcggc ggcgacacca gcagcagtca caactactgt ccacctggac
     3961 aggcggttgt gcctggccag aaatcagccc tcacggagtc ggattcgcag agcagcttcg
     4021 atgcagtgtc gcatcagagc aaggatgagc caccagtcgg gggagtgggt gtggcagtgg
     4081 gtgttgaaca agccctttcc acatctgaca cttcgtcggc ctcctcctca tcgtcatctg
     4141 cttctacagc ttcagggagc tctaatagtt ccacttcctc tatgcttatg gctggacagg
     4201 aggctactgt cggtgttggt ggcgctgtgc agcaaacaac tcgtcgcctc agcaaggatg
     4261 aatcctttag tagcagtagc gacgagttca accagggagg cgtgggtgga tcagcaggtc
     4321 gagtggctat ggcctccagt ggaagcggat cgggtgctgg cgacacagta gcggtgggat
     4381 cgggatcaac agctggcgga gatctgacgc cggtgcccag cacgtcggca gctgcccgtg
     4441 ctgccttggc cgccagctcg acagggccgg tgatttctgc ttcacctcat ctcgcagcta
     4501 gcgccgctgg aggcttagga gtgctgggtg tgggtgcaga ccagccttgc acctcatccg
     4561 cgcatgcagc ccgagcactg gcagctgcag tggctgcttc cagcgataag ccgcatgttt
     4621 tctccaatgt ccggcccaca gaggacgcct gggatatact gctggccaga gccgagggtt
     4681 tgcatgccca cggtcacgga gccgaggctt gcatcctggc cgtccgtctg gccgaacaga
     4741 tgctggcgaa tccgcctaac ctacttctgg aactgccacc cgcacccaaa agaaagggca
     4801 agaagcaaaa cgtgaatccc atctcgcatc agctgacagt tgtggcctct gcaacgctgt
     4861 ccaagtgcgc ttttctgtgc acagtgctgt cggagaactc tgaacactat cacattggct
     4921 ttcggatatg cctattcgcc ttggagatgc cgcgaccgcc agcaagcacc aagcccctgg
     4981 aagtcaagct ggccaaccag gaggcggaca tactggctct actaaagcgc ctgccgctgg
     5041 gatccgcgga actgcaagta atccgagagc gagctgagca actgcgcagc ggaaccttta
     5101 agacgcgagg agaggctcta ttgcctatta acctggccac gtttattttc gatgccctag
     5161 ttacactcag ccccttaggt ggatccacaa ttgtccctgg cgtggcatcc actagtggtg
     5221 tatcaagtgc ggccggcaag tctatggtca atacaggcac acgtttgctg ctctacaagc
     5281 acaacagcga tgagagtctg ggattcgatg ctgcagtggc tgctctcgga ctaaaagcca
     5341 atgtgtcgga ggcagagcat ccgttgttgt gcgaaggaac ccgccggcag cgcggggatc
     5401 ttgctctgac attgctttca cactacaagg atgagccccg caagatagcc aagatcatgg
     5461 agaagcttct ggacagggac atacatacgc ttctaaatgc gccactcctg cccgcttact
     5521 actcgagtaa cccgcctgtg aggacgcgaa gtaatcagcc cacgcgtagg gaggatcacg
     5581 attatggcgg tggctctgga tgcgcctctt ccaactgcaa tccggtagcc aatttgtgtg
     5641 aacttttgcc ggcggactac ggcagtgtgg gtggaaacag tcgaccacac agctccacga
     5701 gtgccgaact ggaactaagc atgtgtgccc tgagcatggc cagcagtggt agccagtcgg
     5761 gaggcgttca aggcatggtg cccacaacga atgcagcggg aaccacggga acacccagtt
     5821 ccagcagcac gacggtcagt gggagccaga atcccaatgg taatcccagt ggcagcggtg
     5881 gaggaggaaa tggcggaggt ggtaacggag gaggaggagg cggtggcggt ggtggtggag
     5941 gctccaccag tagccgaagc aaggaaagcc ggtacaaggg aaagagagca tatccctcga
     6001 tacccaacca gccatcggaa gccagtgctc actttatgtt cgagctggcc aagaatgtgc
     6061 tgaccaaggc cggcggcaat agttccacat cgctcttcac ccaggcgagc acgagccaaa
     6121 atcaccatgg accgcaccgt gccctccaca tgtgcgcctt ccaactaggg ctgtatgccc
     6181 tcggtctgca caactgtgtg agtcccaatt ggctatcgag gacgtactcg tcgcacgtct
     6241 cctggatcct ggggcaagct atggaaattg gtgcgccggc gataagcttt ctgatagaca
     6301 cttgggaggc gcatctaacg cctcccgaag cagctggcat ggccgatcgt gcgtcgcgcg
     6361 gatgggacag caacatggtc tatccggcgg cggagctggc tctgtccgtt ctgccccacg
     6421 cagctgccct caatcccaac gagatccagc gtgcgattct gcagtgcaag gagcaaagcg
     6481 atctgatgct ggagcgggcc tgtctcaccg tggagacagc tgccaagggc ggcggcgtct
     6541 atccggaggt gctattccag gtggcccgct actggtacga gctgtacatg cggaacacgc
     6601 cgaataacag cgagttcgag ccgcacgatg acaccatgga ccattcggcc gtgagtctga
     6661 gcgccctcat tgagtcccag cagcagcatg agctccagca acaacaacag gcagtgcagc
     6721 agcaacaggc ggttcagcag cagcagcagg tggtgcagca acagcagcaa gtggtgcagc
     6781 agcaacaaca gcttggcatg cccaattccg tggtgccagg cggcgttggc cccggccccg
     6841 gactacccgt agcagtgcag ccacccgtag ttggttctgt ccagaatcca caggcccaat
     6901 tccaacccgt gggcgtcgcc tcactggctc ccctcggact ggcgcagtat ccaccgtaca
     6961 gcttctgcca gggcttgtat gcgcaccacc acaacatgag ctatccgccc gggcagatgc
     7021 agatgttcat ttcggcaggg ccgccgccgc cacctcaagc atacggcggc taccagcagc
     7081 cgccgccaca gcagccgccg aatccgcagc aacagcagca ggttgtgcaa cagcaggtgc
     7141 agcagcagca ggtggtgcaa cagcaacagc agcaggttca gatggccggg caggcgccac
     7201 caggtcaccc tgggcagcaa ggacatccag gccaaccgcc ctctggcttc cagccgcaac
     7261 caccgccagg agcattccag gcgctgccgc cgcaggccta tcaggcaatg caagcgggtc
     7321 ccccgggccc gccgatgggt ccgcctcagg gctactatgg tccaccacca ccaccgcctc
     7381 cgaacggtcc gcctggcgtg ggcgtgggcg tcggagtcgg cgttggagtg ggcgtgatgc
     7441 caatgcgcca gcatcagcac caacatcccg tttacccatt catgcagcag gcgccgccgc
     7501 agccaccgca gcagcaacaa ccgccgccct cgcagccacc cgtgcgacaa cgacaaccac
     7561 atcagtttac acccacccag ctgcgatatc tgctggctgc ctacaatgtg ggcatgctgg
     7621 ccatggaaac actggcgcga cgcgtccacg acgatcggcc gcaggcgaag tatgcccgca
     7681 atccgccgta cggcgaggat gtgaaatggt tgctacggat cagcaagaaa ctgggcaccc
     7741 agtatctgca ccagttctgc atctgtgcgg tcaactcgat cgtgagtccg ttcgtgctgc
     7801 atgacgtggc cattgagtcc gcccactatc tgggcaggaa caaccaccag atggtgatgc
     7861 agcacctgcg ttctgcgctc acgcctctcg tccagaagtg tcagcagatg tacatccaat
     7921 gcatccatca gaagctgtat catctgaccc agggcgacta tgaggagttt gccagcattg
     7981 tagtggcggc acgcgccgct ttccagatca cgccggaggg gaacgcgcaa ttcaaggact
     8041 ggctgcagtc tatcaagcga tcgaaatcat gcaaaaagga actgtggacc cagatcaatg
     8101 cggccctgca gagcaactcc aaaaaggccg gccaggcaaa aaagaaaaag ggatcaggca
     8161 agcttgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctg
     8221 ctagcggtag cggcagcggt agcatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     8281 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     8341 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     8401 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     8461 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     8521 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     8581 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     8641 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     8701 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     8761 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     8821 tgacccgcaa gcccggtgcc tgactcgagg gatccagaca tgataagata cattgatgag
     8881 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     8941 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     9001 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     9061 ctctacaaat gtggtatggc tgattatgat cagtcgaccg atgcccttga gagccttcaa
     9121 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     9181 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     9241 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     9301 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     9361 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     9421 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     9481 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     9541 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9601 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9661 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9721 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9781 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9841 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9901 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9961 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10021 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10081 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10141 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10201 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10261 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10321 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10381 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10441 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    10501 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10561 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10621 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
    10681 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10741 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10801 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10861 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10921 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
    10981 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11041 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11101 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11161 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11221 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11281 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    11341 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    11401 tcgtcttcaa gaattaattc tatattctaa aaacacaaat gatacttcta aaaaaaaatc
    11461 atgaatggca tcaactctga atcaaatctt tgcagatgca cctacttctc atttccactg
    11521 tcacatcatt tttccagatc taaaaaagca ccgactcggt gccacttttt caagttgata
    11581 acggactagc cttattttaa cttgctattt ctagctctaa aactacataa cgaataaaaa
    11641 aggatttaag tgcaaatggt aaaaaagtgt cgagtttttc ttgagttgat tgtgctgtaa
    11701 tgagactctg cattcggcat ttgactcggc ttttcctact cgtgccgtat ttcaggctgc
    11761 aagtcgaaca gatctcgctg cctgttatgt ggcccacaaa ccaagacacg ttttatggcc
    11821 attaaagctg gctgatcgtc gccaaacacc aaatacataa tgaatatgta cacattcgag
    11881 aaagaagcga tcaaagaagc gtcttcgggc ggagtaggag aatgcggagg agaaggagaa
    11941 cgagctgatc tagtatctct ccacaatcca atgccaactg accaactggc catattcgga
    12001 gcaatttgaa gccaatttcc atcgcctggc gatcgctcca ttcttggcta tatgtttttc
    12061 accgttaccc ggggccattt tcaaagactc gtcggcaaga taagattgtg tcactcgctg
    12121 tctctcttca tttgtcgaag aatgctgagg aatttcgcga tgacgtcggc gagtattttg
    12181 aagaatgaga ataatttgta tttatacgaa aatcagttag tggaattttc tacaaaaaca
    12241 tgttatctat agataatttt gttgcaaaat atgttgacta tgacaaagat tgtatgtata
    12301 tctttaatgt attctcattt tcttatgtat ttataatggc aatgatgata ctgatgatat
    12361 tttaagatga tgccagacca aaaggcttga atttctgcgt cttttgccga acgcagtgca
    12421 tgtgcaattg ttgttttttg gaatattcaa ttttcggact gtccgctttg atttcagttt
    12481 cttggcttat tcaaaaagca aagtaaagcc aaaaaagcga gatggcaata ccaaatgcgg
    12541 caaaacggta gtggaaggaa aggggtgcgg ggcagcggaa ggaagggtgg ggcgg
//
