LOCUS       sequence-373459-       12427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Puromycin-selectable expression of Dora[T295M] (CG34401) in 
            Drosophila S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Kingston ER, Blodgett LW, Bartel DP
  TITLE     Endogenous transcripts direct microRNA degradation in Drosophila, 
            and this targeted degradation is required for proper embryonic 
            development.
  JOURNAL   Mol Cell. 2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 
            10.1016/j.molcel.2022.08.029.
  PUBMED    36150386
REFERENCE   2  (bases 1 to 12427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 
            10.1016/j.molcel.2022.08.029."
COMMENT     Sequence Label: dora[B]_rescue_construct
FEATURES             Location/Qualifiers
     source          1..12427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7988..8041
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8066..8665
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8066..8085)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8562..8582
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8673..8807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8697..8716)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8751..8770
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9408..9996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9488..9507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10167..10958,10959..11027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10790..10809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11028..11132)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11200..11218
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_RNA        complement(11370..11445)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc atgtgtgcgt
       61 gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat gctgcgctct
      121 ctctagcgcc atctcgctta cgcatgctca acgcaccgca tgttgccgtt tccttttatg
      181 cgtcattttg gctcgaaata ggcaattatt taaacaaaga ttagtcaacg aaaacgctaa
      241 aataaataag tctacaatat ggttacttat tgccatgtgt gtgcagccaa cgatagcaac
      301 aaaagcaaca acacaggtgg ctttccctct ttcacttttt gtttgcaagc cgcgtgcgag
      361 caagacggca cgaccggcaa acgcaattac gctgacaaag agcagacgaa gttttggcga
      421 aaaacatcaa ggcgcctgat acgaatgcat ttgcaataac aattgcgata tttaatattg
      481 tttatgaagc tgtttgactt caaaacacac aaaaaaaaaa ataaaacaaa ttatttgaaa
      541 gagaattagg aatcggacgc ttatcgttag ggtaacaaca agaaatgctt actgagtcac
      601 agcctctgga aaactgccgc aagccagaga gagagagaaa aagagggaga gcagcttaga
      661 ccgcatgtgc ttgtgtgtga ggcgtctctc tcttcgtctc tgttgcgcaa acgcatagac
      721 tgcactgaaa aaatcgatta cctatttttt atgaatgaat atttgcacta ttactattca
      781 aaactattaa gatagcaatc acattcaata gccaaatact ataccacctg agcgatgcaa
      841 cgaaatgatc aatttgagca aaaatgctgc atatttagga cggcatcatt atagaaatgc
      901 ttcttgctgt gtacttttct ctcgtctggc agctgtttcg ccgttattgt taaaaccggc
      961 ttaagttagg tgtgttttct acgactagtg aatgccctac tagaagatgt gtgttgcaca
     1021 aaatgtccct ggaataacca atttgaagtg cagatagcag taaacgtaag ctaatatgaa
     1081 tattatttaa ctgtaatgtt ttaatatcgc tggacattac taataaaccc actataaaca
     1141 catgtacata tgtatgtttt ggcatacaat gagtagttgg ggaaaaaatg tgtaaaagca
     1201 ccgtgaccat cacagcataa agataaccag ctgaagtatc gaatatgagt aacccccaaa
     1261 ttgaatcaca tgccgcaact gataggaccc atggaagtac actcttcatg gcgatataca
     1321 agacacacac aagcacgaac acccagttgc ggaggaaatt ctccgtaaat gaaaacccaa
     1381 tcggcgaaca attcataccc atatatggta aaagttttga acgcgacttg agagcggaga
     1441 gcattgcggc tgataaggtt ttagcgctaa gcgggcttta taaaacgggc tgcgggacca
     1501 gttttcatat cactaccgtt tgagttcttg tgctgtgtgg atactcctcc cgacacaaag
     1561 ccgctccatc agccagcagt cgtctaatcc agagaccccg gatcggggta cctactagtc
     1621 cagtgtggtg gaattcatgg accgcttcag cttcgacgac tccatccgct tcgaggaaga
     1681 ctccctctgc agctggagct ctgaaccgga atcgctgtgc aacaactggc gcggctggaa
     1741 gaagcccgct gcaggcagcg gcgcaggcaa tggcggcttg agcggcatgg gcggtatggc
     1801 tgtggccggc ggctcaggag caccgccgtt cggaggagca ggagcctctg gttcctgcac
     1861 accgggcggc aatggaacgg ccatggccaa cacatccacc tgcggcagac attatgaagt
     1921 atccacattg gccgaactgg cagcccgctg tgtggcctcc tacataccgt tcgagctggt
     1981 tgagcacgtg tatccgccgg tgccggagca gctgcagctg cgcattgcgt tctggagctt
     2041 tccggacaac gaggaggaca tccggctcta ctcctgtctg gccaatagct cggcggacga
     2101 gttcaatcgc ggcgactcgt tgtttcggtt gcgagctgtc aaggatccgc tgcagatagg
     2161 ctttcacctc tcggccagcg ttgtcaatca gacgccgcgt gcctatttca atgtggccgt
     2221 cacctttgac cgccgacgca tctcctcttg caactgcacc tgcacctcgt ccgcctactg
     2281 gtgctcccac gtagtcgccg tttgtctgca tcgcatccat tgcccccagg aggtctgtct
     2341 gcgtgccccg gtatcggaat cattgactcg cctgcaacgc gaccaattgc aaaagtttgc
     2401 ccagtatctg atcagcgagc tgcctcagca gatcttgccg acggctcagc ggctcctgga
     2461 tgagttgctc agtgcgcaac ccacggcgat caacactgtg tgcggtgcac ctgatcccat
     2521 ggctggggcc tcgatcaacg accagaccag ctggtatctg gacgagaaga cgttgcataa
     2581 caacattaaa cgaatcctca tcaagttctg tttgcccgcc ccgattgtgt ttagcgacgt
     2641 aaactatctg acgaactcgg ctcccccagc ggcggccgaa tggagctcgc tactccgacc
     2701 gctgcgggga cgtgagccgg agggcatgtg gaatctactt tcgatcgtac gcgagatgta
     2761 caggcgctgt gatcggaacg cagtgcgtct gctggagatc atcacggagg agtgcttgta
     2821 ctgcgatcag atactcatct ggtggttcca gacgaagttg gcactgatga tggggtccca
     2881 cggccactcc ggcggtaagc attctaacac tcactccaac tccacggcac tgcagcatgc
     2941 ttgcagttcg ctgtgcgacg aaatcgtggc actgtggcga ttggcagcgc tcaatcccgg
     3001 cctggcgccg gatgagcgag atatgctgca cgctcagttc acggcctggc atctaaagat
     3061 tcttgaccgg gtggtcaaga gccgaatgat gccgtcgtac accaacaagc accagcagaa
     3121 ctcgcgatcc gagacggagc tgtttatcgg cttcaagccg gccatcgagg cgtgctactt
     3181 ggactgggag ggctacccca taccgggagt gacgcacacc cacgacacga atcccatcta
     3241 ctactcgccg ttcacttgct tcaagcacac ggacctcaag ggcgagtcta acaatcccgg
     3301 ccagctgaat gccacccaag cgctgatgtc gaacaacaag cactacaatt actttagttc
     3361 atccagcgat catggcatgc acgcaggagc cttcaagcag aggttggacc ggccttttcg
     3421 tgagtcgcga ttctatacga atccggccga tctggacgga ggagcagcgt tggcgggcgg
     3481 aggatccggt aggttttcca cgggactggg ctccgttgcc gttggaggag ctggtgcagg
     3541 aggcttgggt cagatgagcg gaggagttaa caactctgtt ggaggatcag cggccgccga
     3601 agtgaatccc gtgcagcagc agcttcccag tggctcggcg ggagtcggtg cctctgtgac
     3661 aaatgccaag gttgtggtag caacagatgg caatcgttcg agtgccagca gcgagggatt
     3721 ctgcgagaac gacgatttcg gcggcgacac cagcagcagt cacaactact gtccacctgg
     3781 acaggcggtt gtgcctggcc agaaatcagc cctcacggag tcggattcgc agagcagctt
     3841 cgatgcagtg tcgcatcaga gcaaggatga gccaccagtc gggggagtgg gtgtggcagt
     3901 gggtgttgaa caagcccttt ccacatctga cacttcgtcg gcctcctcct catcgtcatc
     3961 tgcttctaca gcttcaggga gctctaatag ttccacttcc tctatgctta tggctggaca
     4021 ggaggctact gtcggtgttg gtggcgctgt gcagcaaaca actcgtcgcc tcagcaagga
     4081 tgaatccttt agtagcagta gcgacgagtt caaccaggga ggcgtgggtg gatcagcagg
     4141 tcgagtggct atggcctcca gtggaagcgg atcgggtgct ggcgacacag tagcggtggg
     4201 atcgggatca acagctggcg gagatctgac gccggtgccc agcacgtcgg cagctgcccg
     4261 tgctgccttg gccgccagct cgacagggcc ggtgatttct gcttcacctc atctcgcagc
     4321 tagcgccgct ggaggcttag gagtgctggg tgtgggtgca gaccagcctt gcacctcatc
     4381 cgcgcatgca gcccgagcac tggcagctgc agtggctgct tccagcgata agccgcatgt
     4441 tttctccaat gtccggccca cagaggacgc ctgggatata ctgctggcca gagccgaggg
     4501 tttgcatgcc cacggtcacg gagccgaggc ttgcatcctg gccgtccgtc tggccgaaca
     4561 gatgctggcg aatccgccta acctacttct ggaactgcca cccgcaccca aaagaaaggg
     4621 caagaagcaa aacgtgaatc ccatctcgca tcagctgaca gttgtggcct ctgcaacgct
     4681 gtccaagtgc gcttttctgt gcacagtgct gtcggagaac tctgaacact atcacattgg
     4741 ctttcggata tgcctattcg ccttggagat gccgcgaccg ccagcaagca ccaagcccct
     4801 ggaagtcaag ctggccaacc aggaggcgga catactggct ctactaaagc gcctgccgct
     4861 gggatccgcg gaactgcaag taatccgaga gcgagctgag caactgcgca gcggaacctt
     4921 taagacgcga ggagaggctc tattgcctat taacctggcc acgtttattt tcgatgccct
     4981 agttacactc agccccttag gtggatccac aattgtccct ggcgtggcat ccactagtgg
     5041 tgtatcaagt gcggccggca agtctatggt caatacaggc acacgtttgc tgctctacaa
     5101 gcacaacagc gatgagagtc tgggattcga tgctgcagtg gctgctctcg gactaaaagc
     5161 caatgtgtcg gaggcagagc atccgttgtt gtgcgaagga acccgccggc agcgcgggga
     5221 tcttgctctg acattgcttt cacactacaa ggatgagccc cgcaagatag ccaagatcat
     5281 ggagaagctt ctggacaggg acatacatac gcttctaaat gcgccactcc tgcccgctta
     5341 ctactcgagt aacccgcctg tgaggacgcg aagtaatcag cccacgcgta gggaggatca
     5401 cgattatggc ggtggctctg gatgcgcctc ttccaactgc aatccggtag ccaatttgtg
     5461 tgaacttttg ccggcggact acggcagtgt gggtggaaac agtcgaccac acagctccac
     5521 gagtgccgaa ctggaactaa gcatgtgtgc cctgagcatg gccagcagtg gtagccagtc
     5581 gggaggcgtt caaggcatgg tgcccacaac gaatgcagcg ggaaccacgg gaacacccag
     5641 ttccagcagc acgacggtca gtgggagcca gaatcccaat ggtaatccca gtggcagcgg
     5701 tggaggagga aatggcggag gtggtaacgg aggaggagga ggcggtggcg gtggtggtgg
     5761 aggctccacc agtagccgaa gcaaggaaag ccggtacaag ggaaagagag catatccctc
     5821 gatacccaac cagccatcgg aagccagtgc tcactttatg ttcgagctgg ccaagaatgt
     5881 gctgaccaag gccggcggca atagttccac atcgctcttc acccaggcga gcacgagcca
     5941 aaatcaccat ggaccgcacc gtgccctcca catgtgcgcc ttccaactag ggctgtatgc
     6001 cctcggtctg cacaactgtg tgagtcccaa ttggctatcg aggacgtact cgtcgcacgt
     6061 ctcctggatc ctggggcaag ctatggaaat tggtgcgccg gcgataagct ttctgataga
     6121 cacttgggag gcgcatctaa cgcctcccga agcagctggc atggccgatc gtgcgtcgcg
     6181 cggatgggac agcaacatgg tctatccggc ggcggagctg gctctgtccg ttctgcccca
     6241 cgcagctgcc ctcaatccca acgagatcca gcgtgcgatt ctgcagtgca aggagcaaag
     6301 cgatctgatg ctggagcggg cctgtctcac cgtggagaca gctgccaagg gcggcggcgt
     6361 ctatccggag gtgctattcc aggtggcccg ctactggtac gagctgtaca tgcggaacac
     6421 gccgaataac agcgagttcg agccgcacga tgacaccatg gaccattcgg ccgtgagtct
     6481 gagcgccctc attgagtccc agcagcagca tgagctccag caacaacaac aggcagtgca
     6541 gcagcaacag gcggttcagc agcagcagca ggtggtgcag caacagcagc aagtggtgca
     6601 gcagcaacaa cagcttggca tgcccaattc cgtggtgcca ggcggcgttg gccccggccc
     6661 cggactaccc gtagcagtgc agccacccgt agttggttct gtccagaatc cacaggccca
     6721 attccaaccc gtgggcgtcg cctcactggc tcccctcgga ctggcgcagt atccaccgta
     6781 cagcttctgc cagggcttgt atgcgcacca ccacaacatg agctatccgc ccgggcagat
     6841 gcagatgttc atttcggcag ggccgccgcc gccacctcaa gcatacggcg gctaccagca
     6901 gccgccgcca cagcagccgc cgaatccgca gcaacagcag caggttgtgc aacagcaggt
     6961 gcagcagcag caggtggtgc aacagcaaca gcagcaggtt cagatggccg ggcaggcgcc
     7021 accaggtcac cctgggcagc aaggacatcc aggccaaccg ccctctggct tccagccgca
     7081 accaccgcca ggagcattcc aggcgctgcc gccgcaggcc tatcaggcaa tgcaagcggg
     7141 tcccccgggc ccgccgatgg gtccgcctca gggctactat ggtccaccac caccaccgcc
     7201 tccgaacggt ccgcctggcg tgggcgtggg cgtcggagtc ggcgttggag tgggcgtgat
     7261 gccaatgcgc cagcatcagc accaacatcc cgtttaccca ttcatgcagc aggcgccgcc
     7321 gcagccaccg cagcagcaac aaccgccgcc ctcgcagcca cccgtgcgac aacgacaacc
     7381 acatcagttt acacccaccc agctgcgata tctgctggct gcctacaatg tgggcatgct
     7441 ggccatggaa acactggcgc gacgcgtcca cgacgatcgg ccgcaggcga agtatgcccg
     7501 caatccgccg tacggcgagg atgtgaaatg gttgctacgg atcagcaaga aactgggcac
     7561 ccagtatctg caccagttct gcatctgtgc ggtcaactcg atcgtgagtc cgttcgtgct
     7621 gcatgacgtg gccattgagt ccgcccacta tctgggcagg aacaaccacc agatggtgat
     7681 gcagcacctg cgttctgcgc tcacgcctct cgtccagaag tgtcagcaga tgtacatcca
     7741 atgcatccat cagaagctgt atcatctgac ccagggcgac tatgaggagt ttgccagcat
     7801 tgtagtggcg gcacgcgccg ctttccagat cacgccggag gggaacgcgc aattcaagga
     7861 ctggctgcag tctatcaagc gatcgaaatc atgcaaaaag gagctgtgga cccagatcaa
     7921 tgcggccctg cagagcaact ccaaaaaggc cggccaggca aaaaagaaaa agggatcagg
     7981 caagcttgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     8041 tgctagcggt agcggcagcg gtagcatgac cgagtacaag cccacggtgc gcctcgccac
     8101 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     8161 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     8221 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     8281 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     8341 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     8401 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     8461 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     8521 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     8581 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     8641 catgacccgc aagcccggtg cctgactcga gggatccaga catgataaga tacattgatg
     8701 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     8761 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     8821 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     8881 acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc
     8941 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     9001 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
     9061 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
     9121 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
     9181 caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg
     9241 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
     9301 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
     9361 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9421 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9481 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9541 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9601 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9661 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9721 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9781 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9841 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9901 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9961 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10021 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    10081 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10141 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10201 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10261 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10321 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10381 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10441 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
    10501 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10561 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10621 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10681 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10741 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
    10801 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10861 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10921 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10981 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    11041 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    11101 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    11161 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    11221 tttcgtcttc aagaattaat tctatattct aaaaacacaa atgatacttc taaaaaaaaa
    11281 tcatgaatgg catcaactct gaatcaaatc tttgcagatg cacctacttc tcatttccac
    11341 tgtcacatca tttttccaga tctaaaaaag caccgactcg gtgccacttt ttcaagttga
    11401 taacggacta gccttatttt aacttgctat ttctagctct aaaactacat aacgaataaa
    11461 aaaggattta agtgcaaatg gtaaaaaagt gtcgagtttt tcttgagttg attgtgctgt
    11521 aatgagactc tgcattcggc atttgactcg gcttttccta ctcgtgccgt atttcaggct
    11581 gcaagtcgaa cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
    11641 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
    11701 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
    11761 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
    11821 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
    11881 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
    11941 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
    12001 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
    12061 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
    12121 tatctttaat gtattctcat tttcttatgt atttataatg gcaatgatga tactgatgat
    12181 attttaagat gatgccagac caaaaggctt gaatttctgc gtcttttgcc gaacgcagtg
    12241 catgtgcaat tgttgttttt tggaatattc aattttcgga ctgtccgctt tgatttcagt
    12301 ttcttggctt attcaaaaag caaagtaaag ccaaaaaagc gagatggcaa taccaaatgc
    12361 ggcaaaacgg tagtggaagg aaaggggtgc ggggcagcgg aaggaagggt ggggcggggc
    12421 gtggcgg
//
